Effect of hypoxia and TP53 mutation status and cytogenetics of normal and malignant mammary epithelium

Vidarsson, Hilmar; Steinarsdóttir, Margrét; Jónasson, Jón Gunnlaugur; Júlíusdóttir, Hildur; Hauksdóttir, Halla; Hilmarsdóttir, Hólmfríður; Halldórsdóttir, Kristín; Ögmundsdóttir, Helga M.

doi:10.1016/j.cancergencyto.2005.07.024

Cited by 1 publication

(1 citation statement)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All other karyotypes were listed complex. Most of the karyotypes have been published previously (Steinarsdottir et al, 1995(Steinarsdottir et al, , 2004Vidarsson et al, 2006;Bodvarsdottir et al, 2007a,b), eight unpublished karyotypes are included here as Supporting Information data (Supporting Information Table 1). Polyclonality was scored for classical cytogenetics, flow cytometry, and combined.…”

Section: Cytogenetic Analysismentioning

confidence: 98%

Cytogenetic polyclonality of breast carcinomas: Association with Clinico‐Pathological Characteristics and Outcome

Steinarsdóttir

Gudmundsson

Jonasson

et al. 2011

Genes Chromosomes & Cancer

View full text Add to dashboard Cite

Routinely used prognostic factors fail to predict clinical outcome in a significant proportion of breast cancer patients, implying that they can not detect some important biological characteristics. Chromosomal changes have been described in breast carcinomas for many years but their significance is not clear. We compared chromosomal changes with clinico-pathological characteristics and clinical outcome in 203 breast cancer patients with a follow-up of 9-18 years. Combining data from classical cytogenetics and flow cytometry revealed chromosomal abnormalities in 142 cases (70%). Of these, 51 (35.9%) contained two or more cytogenetically abnormal clones. Polyclonality was significantly associated with poor breast-cancer-specific survival (P = 0.03) within 5 years, independent of tumor size, lymph node metastases, and hormone receptors. Specific changes were similar to those previously described, but a new finding was a significant association between del 3p12p21 and poor survival. Polyclonality was significantly associated with TP53-mutations but not with a germline BRCA2 mutation. Less than one third of the polyclonal samples were identified by flow cytometry alone. Cytogenetic changes were detected in 17 out of 114 samples from non-tumorous tissue (15%), two of them identical with a clone in the corresponding tumor. Several samples contained clearly unrelated clones within the tumor and outside, implying either multifocal origin or early divergence. In conclusion, the common deletion on Chromosome 3p12p21 was associated with poor clinical outcome. Chromosomal polyclonality is common in breast carcinomas and predicts poor survival. Polyclonality was poorly detected by one-sample flow cytometry. Multiple sampling might improve the detection rate.

show abstract

Section: Cytogenetic Analysismentioning

confidence: 98%