Effect of Hypoxia on Formation of Three-Dimensional Microvessel Networks by Endothelial Cells in vitro

Ueda, Akinori; Sudo, Ryo; Ikeda, Mariko; Kokubo, K.; Kudo, Susumu; Kobayashi, Hirosuke; Tanishita, Kazuo

doi:10.1299/jbse.3.299

Cited by 3 publications

(3 citation statements)

References 17 publications

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“…Oxygen tension is a well-known regulator of neo-vascularization through cellular self-assembly of MSCs and HUVECs. [73,74] One report investigated endothelial cells, that when seeded onto collagen gels, showed increased sprouting into the collagen gel under hypoxic conditions as compared to normoxic conditions. [73] Another report demonstrated that reducing oxygen concentration to 1% reduced angiogenic sprouting of endothelial cells seeded on top of fibrin hydrogels.…”

Section: Effect Of Delayed Introduction Of Microchannels Within Cell-...mentioning

confidence: 99%

“…[73,74] One report investigated endothelial cells, that when seeded onto collagen gels, showed increased sprouting into the collagen gel under hypoxic conditions as compared to normoxic conditions. [73] Another report demonstrated that reducing oxygen concentration to 1% reduced angiogenic sprouting of endothelial cells seeded on top of fibrin hydrogels. [74] It is therefore likely that the enhanced capillary-like network formation within the delayed dissolution samples was due to the temporally reduced oxygen availability.…”

Section: Effect Of Delayed Introduction Of Microchannels Within Cell-...mentioning

confidence: 99%

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Programming Delayed Dissolution Into Sacrificial Bioinks For Dynamic Temporal Control of Architecture within 3D‐Bioprinted Constructs

Soliman

Longoni

Wang

et al. 2023

Adv Funct Materials

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Sacrificial printing allows introduction of architectural cues within engineered tissue constructs. This strategy adopts the use of a 3D‐printed sacrificial ink that is embedded within a bulk hydrogel which is subsequently dissolved to leave open‐channels. However, current conventional sacrificial inks do not recapitulate the dynamic nature of tissue development, such as the temporal presentation of architectural cues matching cellular requirements during different stages of maturation. To address this limitation, a new class of sacrificial inks is developed that exhibits tailorable and programmable delayed dissolution profiles (1–17 days), by exploiting the unique ability of the ruthenium complex and sodium persulfate initiating system to crosslink native tyrosine groups present in non‐chemically modified gelatin. These novel sacrificial inks are also shown to be compatible with a range of biofabrication technologies, including extrusion‐based printing, digital‐light processing, and volumetric bioprinting. Further embedding these sacrificial templates within cell‐laden bulk hydrogels displays precise control over the spatial and temporal introduction of architectural features into cell‐laden hydrogel constructs. This approach demonstrates the unique capacity of delaying dissolution of sacrificial inks to modulate cell behavior, improving the deposition of mineralized matrix and capillary‐like network formation in osteogenic and vasculogenic culture, respectively.

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Section: Effect Of Delayed Introduction Of Microchannels Within Cell-...mentioning

confidence: 99%

Section: Effect Of Delayed Introduction Of Microchannels Within Cell-...mentioning

confidence: 99%

Programming Delayed Dissolution Into Sacrificial Bioinks For Dynamic Temporal Control of Architecture within 3D‐Bioprinted Constructs

Soliman

Longoni

Wang

et al. 2023

Adv Funct Materials

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show abstract

“…2A, upper panel). Hypoxia conditions can also promote the formation of 3D vascular networks within a collagen matrix by increased gene expression of angiogenic factors, such as vascular endothelial growth factor (VEGF) families and FGF (Ueda et al, 2008). Endothelial progenitor cells (EPCs) also contributed to 3D vascular network formation by secreting angiogenic factors, such as VEGF (Koga et al, 2009;Abe et al, 2013a).…”

Section: In Vitro Angiogenesis Modelsmentioning

confidence: 99%

Progress and challenges in vascular tissue engineering using self-organization/pre-designed approaches

Watanabe

Sudo

2021

JBSE

Self Cite

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Progress and challenges in vascular tissue engineering using self-organization/pre-designed approaches IntroductionCurrently, organ transplantation is the most effective therapy for end-stage organ failure. However, the demand for life-saving organ transplants far exceeds the supply of available organs, including the kidney, liver, heart, and lung owing to organ shortage (Lauerer et al., 2016). To address this problem, tissue engineering has offered potential strategies for the construction of solid tissues and organs using cells, signaling factors, and scaffolds (Langer and Vacanti, 1993). Over the last several decades, various approaches have been developed for the construction of organ-specific tissues by utilizing cell culture platforms, such as porous scaffolds and decellularized matrices, and scaffold-free culture methods, including cell sheet engineering and spheroid culture (Uygun et al., 2010;Bao et al., 2011;No et al., 2012;Damania et al., 2014). Despite great progress, limitations remain for medical and clinical applications of tissue-engineered constructs owing to the lack of functional vascular networks, which may result in tissue dysfunction, such as hemorrhage, clot formation, and tissue hypoxia.Functional vascular networks should be constructed in tissue-engineered constructs. Establishing a hierarchical tubular network is required to supply oxygen and nutrients to the tissue constructs. In particular, following morphological

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Three-Dimensional Network Formation of Endothelial Cells Depended on Shear Stress(Fluids Engineering)

Abe

Sudo²,

Ikeda³

et al. 2010

Trans.JSME, Ser.B

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Effect of Hypoxia on Formation of Three-Dimensional Microvessel Networks by Endothelial Cells in vitro

Cited by 3 publications

References 17 publications

Programming Delayed Dissolution Into Sacrificial Bioinks For Dynamic Temporal Control of Architecture within 3D‐Bioprinted Constructs

Programming Delayed Dissolution Into Sacrificial Bioinks For Dynamic Temporal Control of Architecture within 3D‐Bioprinted Constructs

Progress and challenges in vascular tissue engineering using self-organization/pre-designed approaches

Three-Dimensional Network Formation of Endothelial Cells Depended on Shear Stress(Fluids Engineering)

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