2022
DOI: 10.56093/ijans.v92i10.104284
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Effect of hypoxia on production of caprine chimeric blastocyst

Abstract: This study was conducted to evaluate the effect of 5% and 21% oxygen concentrations on chimeric embryo development. Inner cell mass (ICM) from parthenogenetic activated blastocyst were used to produce ES cell-like cells. A pair of zona-free tetraploid embryos and one clump of pESCs at passage 2 and 3 were aggregated on granulosa monolayer in such a manner that the clump of pESCs was sandwiched between the tetraploid embryos. The aggregates prepared were randomly divided into two groups viz. Group 1 (21% O2) (n… Show more

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