2016
DOI: 10.9790/0853-150807102111
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Effect of Hypoxia on Stemness and Differentiation of Dental Pulp Derived Stem Cells

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Cited by 8 publications
(10 citation statements)
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“…For enrichment of exosomes, these hMSCs were propagated in serum-free media (STEMPRO ® MSC SFM CTS, Thermo Fisher Scientific) for 48 h. The hMSCs were stained for tri-lineage (adipocytes, osteocytes, and chondrocytes) and specific surface markers for flow cytometry which include CD105, CD73, CD29 and CD90, HLA-I, CD34/45, and HLA class II. Selection of these markers and flow cytometry were carried out in accordance with described protocols [ 13 , 14 ].…”
Section: Methodsmentioning
confidence: 99%
“…For enrichment of exosomes, these hMSCs were propagated in serum-free media (STEMPRO ® MSC SFM CTS, Thermo Fisher Scientific) for 48 h. The hMSCs were stained for tri-lineage (adipocytes, osteocytes, and chondrocytes) and specific surface markers for flow cytometry which include CD105, CD73, CD29 and CD90, HLA-I, CD34/45, and HLA class II. Selection of these markers and flow cytometry were carried out in accordance with described protocols [ 13 , 14 ].…”
Section: Methodsmentioning
confidence: 99%
“…Dental pulp derived hMSCs were obtained from the third molar of each individual (16–18 years) who came for orthodontic treatment at the Department of Orthodontics and Dento-Facial Deformities, Centre for Dental Education and Research (CDER), AIIMS, New Delhi. Briefly, DP-MSCs were isolated and cultured as previously described protocol [ 24 , 25 ].…”
Section: Methodsmentioning
confidence: 99%
“…Proliferation rate of hMSCs ( N = 3) was performed at days 1, 3, 5, 7, and 14 and measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma, USA) assay. The technique was performed as per the previous established protocol [ 12 ].…”
Section: Methodsmentioning
confidence: 99%
“…Features of altered BM microenvironment have been described in acquired aplastic anemia [5,9]. Previous studies on BM-MSCs in aAA have shown equivocal outcomes, such as abnormal morphology [10,11], lower population doubling time, and poor proliferation and differentiation capacity of aAA BM-MSCs [10,[12][13][14][15][16][17], whereas others have found no differences in aAA BM-MSCs as compared to normal BM-MSCs [18][19][20]. us, the exact picture of BM microenvironment and its role in the disease need investigation.…”
Section: Introductionmentioning
confidence: 99%