Effect ofBauhinia racemosaStem Bark on N-nitrosodiethylamine-Induced Hepatocarcinogenesis in Rats

Abstract

The aim of this study is to investigate the antioxidant defense system induced by the methanol extract of Bauhinia racemosa L.(MEBR) against N-nitrosodiethylamine (NDEA)-induced hepatocarcinogenesis in Wister albino rats. The effects of MEBR on surface visible macroscopic (Morphometry) liver lesions (neoplastic nodules) and the levels of serum enzymes, lipid peroxidation and antioxidants were evaluated in NDEA-induced hepatocarcinogenesis in rats. In rats treated, with NDEA, significantly elevated levels of serum enzymes (SGOT, SGPT and ALP), bilirubin and decreased levels of protein and uric acid were observed. Significantly elevated amount of malondialdehyde (MDA), the end product of lipidperoxidation, indicated higher levels of lipid peroxidation, which was accompanied by significantly decreased levels of antioxidants like vitamin C, vitamin E, reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT). Administration of MEBR was able to suppress nodule development/hepatocellular lesion formation in rats. The extract treatment increases in antioxidant levels and dramatic decreases in lipid peroxidation levels. MEBR also produced a protective effect by decreasing the level of serum enzymes, bilirubin and increased the protein and uric acid levels. The results suggest that MEBR exert chemopreventive effects by suppressing nodule development and decreasing lipid peroxidation and enhancing the levels of antioxidants in NDEA carcinogenesis by reducing the formation of free radicals.

Targeting specific cell signaling transduction pathways by dietary and medicinal phytochemicals in cancer chemoprevention

“…A number of studies have indicated that antioxidant rich plant-derived components and extracts can be considered as prophylactic agents in cancer. Plant extracts with high antioxidant index can mediate at least in part their anticarcinogenic effect by acting as free radical scavengers and metal chelators (Cheung and Tai, 2007;Ju et al, 2004;Kumar et al, 2007;Lee et al, 2004a,b;Moongkarndi et al, 2004;Neergheen et al, 2006;Soobrattee et al, 2008) (Table 1). Damage to the biomolecules disrupt important metabolic pathways by inducing genetic alterations in genes involved in carcinogenesis (e.g.…”

Medicinal uses, phytochemistry and pharmacology of Bauhinia racemosa lam

“…Petroleum ether, chlolroform, ethylacetate, methanol Anthelmintic [26] Antibacterial [27] Ethanol, n-hexane, chloroform, n-Butanol Antifilarial [28] Aqueous, methanol Antihyperglycaemic [29] Ethanol Antihistaminic [9] , Anti-inflammatory, Antidiarrhoeal [30] Antispasmodic [31] Bark Alcoholic Antipyretic [32] Aqueous and alcoholic Antiulcer [32] Methanol Antitumor [8] Analgesic [15] Anti-inflammatory [33] Anti-HIV activity [34] Antioxidant, Hepatoprotective [35] Fruit Aqueous, alcoholic Antiulcer [33] Whole plant Petroleum ether, ethanol, aqueous Antihistaminic [9] Aqueous, alcoholic Antihyperglycemic [36] Ethanol Antihelmintic [10] Methanolic Anxiolytic [7] , Antitumor [37] Table 7: Bioactive phytochemicals present in various parts of Bauhinia racemosa Lam…”

Protective Effect of Bauhinia Racemosa Against Streptozotocin Induced Diabetes Male Infertility Complications

“…Bauhinia racemosa belongs to the family Fabaceae, its leaf extract having anti-inflammatory, analgesic, anti-pyretic 5 , skin diseases, anti-spasmodic, anti-helmenthetic and antimicrobial activity 6 . Anti-oxidant, anti-tumor 7,8 and hepato-protective effects 9 . A bark extract is widely useful for ulcers, tooth and inflammation.…”