2010
DOI: 10.1128/iai.00464-10
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Effect of Borrelia burgdorferi OspC at the Site of Inoculation in Mouse Skin

Abstract: The Borrelia burgdorferi surface lipoprotein OspC is a critical virulence factor, but its precise role in the establishment of B. burgdorferi infection remains unclear. To determine whether OspC affects the host response at the site of inoculation of the bacterium, the recruitment of macrophages and neutrophils and the production of cytokines were examined at the site of infection by wild-type, ospC mutant, and complemented mutant B. burgdorferi strains. Of the 21 cytokines tested, monocyte chemoattractant pro… Show more

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Cited by 29 publications
(23 citation statements)
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“…Although previous reports showed that neutrophils effectively control spirochete burden in tissues from mice infected with wildtype spirochetes (74,75), our data indicated that neutrophils were not the major factor in clearing the ospC mutant at the skin site of inoculation in mice. This finding is consistent with a previous study showing no differences in neutrophil infiltration in skin biopsy specimens from mice challenged with wild-type or ospC mutant spirochetes (59). In contrast, we found that depletion of mononuclear phagocytes at the skin site of inoculation in SCID and C3H/HeN mice allowed the ospC mutant to establish infection and dissemination, suggesting that these innate cells were indispensable in clearing the ospC mutant.…”
Section: Discussionsupporting
confidence: 93%
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“…Although previous reports showed that neutrophils effectively control spirochete burden in tissues from mice infected with wildtype spirochetes (74,75), our data indicated that neutrophils were not the major factor in clearing the ospC mutant at the skin site of inoculation in mice. This finding is consistent with a previous study showing no differences in neutrophil infiltration in skin biopsy specimens from mice challenged with wild-type or ospC mutant spirochetes (59). In contrast, we found that depletion of mononuclear phagocytes at the skin site of inoculation in SCID and C3H/HeN mice allowed the ospC mutant to establish infection and dissemination, suggesting that these innate cells were indispensable in clearing the ospC mutant.…”
Section: Discussionsupporting
confidence: 93%
“…Our results showed that the ospC mutant also was not able to establish infection in NODSCIDg mice when inoculated at the standard dose (10 3 spirochetes per mouse). The same inoculum of ospC mutant spirochetes cannot cause infection in C3H/HeN and SCID mice, which is what we observed in our infectivity experiments using these mouse strains (8,17,21,59). Our findings suggest that the lytic complement and NK cells were dispensable in the clearance of the ospC mutant.…”
Section: Discussionsupporting
confidence: 83%
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“…While specific molecular functions of OspC are not yet clear, it is known that ospC is required for host infection and is among the most differentially expressed genes during host invasion (Brooks et al 2003;Grimm et al 2004;Liang et al 2004;Tilly et al 2006;Antonara et al 2010). Considering that ospC is a serotype determinant of B. burgdorferi s.l., negative FDS operating at ospC is highly plausible.…”
Section: Maintenance Of Genome Clusters By Frequency-dependent Selectionmentioning
confidence: 99%