Effect of Mycoplasma agalactiae mastitis on milk production and composition in Valle dell Belice dairy sheep

Tolone, Marco; Sutera, Anna Maria; Borrello, Silvio; Tumino, Serena; Scatassa, Maria Luisa; Portolano, Baldassare; Puleio, Roberto; Nicholas, R. A. J.; Loria, Guido Ruggero

doi:10.1080/1828051x.2019.1617044

Cited by 6 publications

(3 citation statements)

References 16 publications

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“…The problem is compounded by the fact that physiological monitoring as a herd health management tool does not apply for CA. Indeed, in active outbreaks, somatic cell counts (SCC) may increase in correlation with mammary signs80,102,103 but remain in normal ranges in chronically infected herds 47,85,104,105. Other milk quality traits are affected similarly by mastitis, whatever the etiological agent 106…”

Section: Diagnosismentioning

confidence: 99%

Contagious Agalactia In Sheep And Goats: Current Perspectives

Jaý¹,

Tardy²

2019

VMRR

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Contagious agalactia (CA) is a disease caused equally by four Mycoplasma species, in single or mixed infections. Clinical signs are multiple, including mastitis, arthritis, keratoconjunctivitis, pneumonia, and septicemia, non-specific, and expressed differently depending whether sheep or goats are affected, on causative mycoplasmas as well as type of husbandry. CA has been reported worldwide and its geographic distribution maps to that of small ruminant breeding areas. However, as current diagnostic tests are expensive and difficult to implement, it is certainly underdiagnosed and prevalence data are only available for a few countries. CA control relies on vaccines, chemotherapy and good herd management practices. It requires long-term commitment but is often unsuccessful, with frequent clinical relapses. The persistence of the etiological agents, despite their overall susceptibility to antimicrobials, comes from their genetic plasticity and capacity to escape the host immune response. The existence of asymptomatic carriers and the numerous sources of infections contribute to rapid spread of the disease and complicate the control and prevention efforts. Here we review all these aspects in order to highlight recent progress made and identify gaps in knowledge or tools needed for better disease management. Discussion also underlines the detrimental effect of contagious agalactia on small ruminant welfare.

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Section: Diagnosismentioning

confidence: 99%

Contagious Agalactia In Sheep And Goats: Current Perspectives

Jaý¹,

Tardy²

2019

VMRR

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“…In most cases, mycoplasmas affect the respiratory system, reproductive tract, cause severe septicemia, affecting internal organs. Mycoplasmas are capable of causing an infectious process in mammals exclusively in association with other microorganisms (Tolone et al, 2019). Several mycoplasma species have an immunosuppressive effect, which manifests itself in the selective suppression of cellular immunity.…”

Section: Introductionmentioning

confidence: 99%

Clinical and Pathoanatomical Aspects of Combined Chlamydial-Mycoplasma-Klebsiella Infection of Lambs

Metleva¹,

Smolovskaya²,

Semechkova³

et al. 2021

J Biochem Technol

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An autopsy of lambs that died during the first two days after birth was carried out. Typical clinical disease signs were refusal to milk, lethargy, rounded formations in the middle third part of the neck measuring 11×7×8 (cm), and death. An autopsy revealed an acute expansion and paralysis of the heart, as a result of which congested phenomena in the organs in the pulmonary circulation (pulmonary and cerebral edema) were established; hemorrhagic inflammation of the kidneys, lymphoidulitis of deep cervical and mesenteric lymph nodes, acute hemorrhagic enterocolitis. The death of the lambs was caused by heart paralysis. In the process of the autopsy, samples of pathological material (lymph nodes, heart, lungs, liver, kidneys, spleen, and brain) were taken aseptically. The samples were subjected to PCR-diagnostics and microbiological studies at the Research Laboratory of Biochemical, Molecular Genetic Research, and Breeding of Farm Animals of the Kuzbass State Agricultural Academy; they were studied for the presence of infectious agents that can be transmitted transplacentally. As a result of studies of the internal organs of lambs, the presence of the following microorganisms was established: K. pneumoniae, Chlamydiacеae spp., Mycoplasma spp. The ability of K.pneumoniae to grow under anaerobic conditions, with the production of hemolysin, was studied as well, which indicates the ability to penetrate and develop in eukaryotic cells, incl. in intrauterine conditions.

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“…Clinical signs are characterized by mastitis, arthritis, keratoconjunctivitis and occasionally, abortion. The course of the disease starts as mild monolateral interstitial mastitis, with warm, swollen, painful parenchyma followed by sclerosis of the udder, alteration in the quality of milk with a drastic decline in dairy production [1][2][3].…”

Section: Introductionmentioning

confidence: 99%

Validation of Loop-Mediated Isothermal Amplification (LAMP) Field Tool for Rapid and Sensitive Diagnosis of Contagious Agalactia in Small Ruminants

Tumino

Tolone

Parco

et al. 2020

Animals

Self Cite

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Contagious agalactia (CA), an infectious disease of small ruminants, caused by Mycoplasma agalactiae, is responsible for severe losses to dairy sheep production with substantial socioeconomic impacts on small-scale farmers. The diagnosis of CA is still problematic, time-consuming and requires well-equipped labs for confirmation of outbreaks. Therefore, rapid, accurate and cost-effective diagnostic tests are urgently needed. This work aims to validate a novel Loop-Mediated Isothermal Amplification (LAMP) test, based on the p40 target gene, for the detection of M. agalactiae in dairy sheep in order to confirm its potential practical use as a rapid and cheap field test. The LAMP system proposed in this study consists of a portable device composed of real-time fluorometer with the automatic interpretation of results displayed in a tablet. A total of 110 milk samples (90 positives and 20 negatives) were analysed to optimise the analysis procedure and to investigate the efficacy and robustness of the LAMP method. All samples were analysed using LAMP and conventional real-time PCR to compare the diagnostic sensitivity of the methods. The sensitivity of the LAMP was 10-fold higher than that of real-time PCR, with a detection limit up to 103 CFU/ml. The LAMP assay was able to detect M. agalactiae in 81 of 90 (90%, 95%CI 0.84–0.96) positive milk samples compared to 69 (77%, 95%CI 0.59–0.95) positive samples detected by real-time PCR; no positive signal occurred for any of the negative milk samples in either test. Therefore, the LAMP assay was found to be more sensitive than real-time PCR, low-cost, easy to perform, fast and not affected by contamination, indicating its potential as an effective diagnostic tool in the field level for the diagnosis of CA.

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Effect of Mycoplasma agalactiae mastitis on milk production and composition in Valle dell Belice dairy sheep

Cited by 6 publications

References 16 publications

<p>Contagious Agalactia In Sheep And Goats: Current Perspectives</p>

<p>Contagious Agalactia In Sheep And Goats: Current Perspectives</p>

Clinical and Pathoanatomical Aspects of Combined Chlamydial-Mycoplasma-Klebsiella Infection of Lambs

Validation of Loop-Mediated Isothermal Amplification (LAMP) Field Tool for Rapid and Sensitive Diagnosis of Contagious Agalactia in Small Ruminants

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