The health benefits of eicosapentaenoic acid (EPA)-enriched phospholipids (PL) have witnessed a recent upsurge. In the present study, PL from starfish Asterias amurensis (SFP) and sea cucumber Cucumaria frondosa (SCP) were extracted and the liposomes were prepared. Both SFP and SCP liposomes showed antitumour effects in vitro and exhibited high transport and uptake effects in small intestinal epithelial cell models. The results also demonstrated that dietary SFP and SCP liposomes prolonged the life span and ameliorated oxidative stress status of S180 ascitic tumourbearing mice. Results also showed that dietary SFP and SCP liposomes up-regulated the expression of Bax and down-regulated Bcl-2 level in tumour cells, then induced release of cytochrome c from mitochondria to cytosol, thus activation of caspase 9 and caspase 3. Taken together, these findings suggest that SFP and SCP liposomes exert antitumour effects mainly via activation of mitochondrial apoptotic pathways in tumour-bearing mice.
Dear Dr. SeiberOn behalf of my co-authors, we thank you so much for giving us an opportunity to revise our manuscript. We really appreciate your comments and suggestions on our manuscript entitled "Antitumour activity of EPA-enriched phospholipids liposomes against S180 ascitic tumour-bearing mice" (ID: JFF-D-15-01043). Those comments are all valuable and very helpful for revising and improving our paper, as well as the important guiding significance to our research.We have studied your comments carefully and have tried our best to revise our manuscript according to the comments. We have made revision which marked in red in the paper.We would like to express our great appreciation to you for comments on our paper.Thank you and best regards.
List of changes and responsesResponds to the Editor's comments:Editor: There are changes that must be made before I can pass it on to the production people. It is essential that all marked points be addressed in order to avoid delays in the publication.
Response:We have made correction according to the Editor's comments. In order tohighlight the change what we have done, the modified parts have been marked in red in the revised manuscript.About the questions in P7 and P15, analysis of molecular species of phospholipids in starfish and sea cucumber used in this study shows that EPA is often located in the sn-2 position of phospholipids. In addition, other polyunsaturated fatty acids such as DHA and AA are also incorporated into the sn-2 position. And the saturated chain such as C18:0 (stearic acid) is always in the sn-1 position (data not shown).Finally, about the question in P11, we are so sorry that we failed to find the approved number. But we carried out the guidelines of Ethical Committee of Experimental Animal Care strictly.We really appreciate your comments and suggestions. 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 -3 -
AbstractThe...