Effect ofTinospora cordifoliaon the reduction of ultraviolet radiation‐induced cytotoxicity and DNA damage in PC12 cells

Abstract

The safety of Tinospora cordifolia and its potential to protect against ultraviolet radiation-induced cytotoxicity and DNA damage in PC12 cells were investigated. To evaluate the safety of T. cordifolia, cell viability, and agarose gel electrophoresis were carried out using PC12 cells treated with 0 to 100 µg mL -1 of methanol extract of T. cordifolia. T. cordifolia extracts did not show cytotoxicity ranging 0 to 100 µg mL -1 . In addition, T. cordifolia extracts significantly increased cell viability at 1 ng, 10 ng, and 1 µg mL -1 concentrations in serum-deprived medium compared to control. To confirm the protective role against UV-induced damage, PC12 cells alone or in presence of 10 ng, 100 ng, or 1 µg mL -1 of T.cordifolia extract were exposed to 250, 270, and 290 nm of UV radiation, which corresponded to doses of 120, 150 and 300 mJ cm -2 , respectively. Treatment with T. cordifolia extracts significantly increased the cell survival rate irradiated at 290 nm. In addition, T. cordifolia extracts significantly reduced cyclobutane pyrimidine dimer formation induced by UV irradiation at all wavelengths. In conclusion, T. cordifolia is not toxic and safe for cells. Our findings can support its application as phototherapy in the medical sector.

Telomeres, DNA Damage and Ageing: Potential Leads from Ayurvedic Rasayana (Anti-Ageing) Drugs

“…It is used at several dosage forms to treat inflammation, arthritis, allergy, diabetes and as an anti-ageing and rejuvenating tonic [14][15][16]. A study found that extracts from Guduchi markedly enhanced the rate of cell survival and protected against radiation-induced cytotoxicity and DNA damage in PC12 cells [17]. Another study using ethanolic Guduchi stem extracts reported DNA protective ability on sodium arsenite-induced genotoxicity in lymphocytes from Swiss Albino mice using the comet assay [18].…”