Effect of IgG from multiple sclerosis patients on amidolytic activity of coagulation and anticoagulation factors of hemostasis

Katrii, T.; V.Yu., Shandyuk; Vovk, Tetiana; Halenova, Tetiana; Raksha, Nataliia; Shershnov, O V; Melnyk, Volodymyr; Savchuk, О.; Остапченко, Л. И.

doi:10.15419/bmrat.v4i08.205

Cited by 6 publications

(2 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some studies have described the synthesis of antibodies against coagulation components in patients with multiple sclerosis. These antibodies are specific for anticoagulant mediators and serine proteases [29]. We assume that the immune system is able to produce antibodies against abnormal hemostasis components as a result of the violation of proteolysis during post-COVID-19 conditions.…”

Section: Discussionmentioning

confidence: 99%

A study of fibrinolytic system components in donor groups depending on various titers of circulating anti-SARS-CoV-2 IgG in the bloodstream

Rachkovska

Krenytska

Karbovskyy

et al. 2023

Blood Coagulation &Amp; Fibrinolysis

View full text Add to dashboard Cite

The fibrinolytic system plays an important role in controlling blood coagulation at each stage, from thrombin generation to fibrin clot cleavage. Currently, long-term multiorgan dysfunction post-coronavirus disease 2019 (COVID-19) may include coagulation disorders. Little information is available about the potential causes of post-COVID-19 coagulopathy, but one of them may be subpopulation IgG produced by the immune system against SARS-CoV-2. This article describes the changes in the main parameters of the fibrinolytic system in donors with various titers of anti-SARS-CoV-2 IgG, which is part of a complex study of the hemostasis system in these donor groups. We determined the most significant parameters of the fibrinolytic system, such as potential activity and amount of plasminogen and tissue plasminogen activator (tPA), amount of plasminogen activator inhibitor-1 (PAI-1), inhibitory potentials of α-2-antiplasmin, α-1-antitrypsin, α-2-macroglobulin in the blood plasma of donor groups. The obtained results represent the maximum and minimum values of measurement parameters among donor groups with titers of anti-SARS-CoV-2 IgG at least 10 ± 3 Index (S/C), and their statistical differences from the reference point [donor group with titer of anti-SARS-CoV-2 IgG 0 Index (S/C)]. We established the changes in fibrinolytic parameters depending on the titers of anti-SARS-CoV-2 IgG. One conclusion can be drawn from this: anti-SARS-CoV-2 IgG population may influence coagulation in the post-COVID-19 period. Further research in-vitro and in-vivo experimental models using selected and purified IgG may confirm our previous findings.

show abstract

Section: Discussionmentioning

confidence: 99%

A study of fibrinolytic system components in donor groups depending on various titers of circulating anti-SARS-CoV-2 IgG in the bloodstream

Rachkovska

Krenytska

Karbovskyy

et al. 2023

Blood Coagulation &Amp; Fibrinolysis

View full text Add to dashboard Cite

show abstract

“…Currently, the development of coagulation disorders is a known consequence of diseases such as viral hepatitis, Epstein-Barr infection, systemic sclerosis, and ischemic stroke by accumulation of autoantibodies. [17][18][19][20] A recent study has reported that autoantibodies can be detected in COVID-19 patients with three types being identified: antiphospholipids, anticytoplasmic neutrophils, and antinuclear antibodies. 21 We determined the dependence of anticoagulation parameters and titers of anti-SARS-CoV-2 IgG in donors who had recovered from COVID-19.…”

Section: Discussionmentioning

confidence: 99%

Coagulation Parameters in Post-Covid-19 Condition in Relation to Various Titers of Anti-SARS-CoV-2 IgG in Blood Plasma

Strubchevska,

Rachkovska,

Krenytska

et al. 2023

IJGM

View full text Add to dashboard Cite

Background and Objectives Post-COVID-19 condition is thought to affect 10–20% of people at least 3 months after a diagnosis of COVID-19 and two months of symptoms. Post-COVID-19 condition presents itself with many clinical effects with varying degrees of severity ranging from a mild cough to a life-threatening coagulopathy. Our study aimed to identify a relationship between the titers of anti-SARS-CoV-2 IgG and anticoagulation parameters: antithrombin III (ATIII), protein C (PC) and thrombomodulin (TM). Materials and Methods Blood plasma was collected from healthy donors aged 25–45 who had recovered from COVID-19 3–6 months ago and their titers of anti-SARS-CoV-2 IgG and ATIII, PC, and TM were measured. Results We found that concentrations and activities of key anticoagulation parameters (ATIII, PC, and TM) measured in donor plasma during the post-COVID-19 varied in relation to the titers of anti-SARS-CoV-2 IgG. Conclusion While we identified a dysfunction of anticoagulation parameters in patients with post-COVID-19, we aim to explore the subpopulation antibody IgG fraction directly using in vivo and in vitro experiments with the possibility to contribute to the development of treatment options for post-COVID-19 conditions.

show abstract

Characteristics of Products of Fibrinogen Origin in the Presence of Anti- SARS-CoV-2 IgG in the Bloodstream

Rachkovska

Krenytska

Karbovskyy

et al. 2023

RRCT

View full text Add to dashboard Cite

Background: The hemostasis system has been extensively investigated in patients in the acute phase of coronavirus disease 2019 (COVID-19). In contrast, the post-COVID syndrome is a poorly known entity, and there is a lack of information on the mechanisms underlying the hemostasis abnormalities in the post-COVID period. Aim: To analyze the potential changes in the parameters of the hemostasis system in the post-COVID period in the plasma of donors with different titers of anti-SARS-CoV-2 IgG. Methods: The plasma from 160 donors who had recovered from COVID infection was used in the study. Based on the results of the Abbott SARS-CoV-2 IgG serological assay, all donors were divided into several groups: 5 ± 3 (n=20); 55 ± 5 (n=20); 65 ± 5 (n=20); 75 ± 5 (n=20); 85 ± 5 (n=20); 95 ± 5 (n=20); 125 ± 5 (n=20); 175 ± 5 (n=20) Index (S/C). A total of 20 healthy individuals without anti-SARS-CoV-2 IgG constituted the control group. Key laboratory parameters, such as fibrinogen concentrations, soluble fibrin monomer complex (SFMCs), and D-dimer, were investigated. In addition, the qualitative composition of the fraction of SFMCs was analyzed. Results: The slight increase in the concentration of fibrinogen, SFMCs, and D-dimers in some donor groups have been found, which could cause the development of hemostasis disorders. In the fraction of SFMCs, the increase in the number of protein fragments with a molecular weight of less than 250 kDa and an increase in the level of proteins with a molecular weight of more than 270 kDa was revealed. Conclusion: The obtained results indicated the relationship between the changes in the parameters of the hemostasis system and the titers of anti-SARS-CoV-2 IgG in donors in the post-COVID period. It can be assumed that donors with higher titers of anti-SARS-CoV-2 IgG (>55 ± 5 Index (S/C)) are more prone to hemostasis abnormalities in the post-COVID period since a pronounced imbalance in the levels of SFMCs and D-dimer characterizes them. The appearance of protein fragments of different molecular weights in the fraction of SFMC points to uncontrolled activation of biochemical processes involving molecules of fibrinogenic origin. Additional studies are required to elucidate the role of anti-SARS-CoV-2 IgG in the post-COVID period.

show abstract

Effect of IgG from multiple sclerosis patients on amidolytic activity of coagulation and anticoagulation factors of hemostasis

Cited by 6 publications

References 18 publications

A study of fibrinolytic system components in donor groups depending on various titers of circulating anti-SARS-CoV-2 IgG in the bloodstream

A study of fibrinolytic system components in donor groups depending on various titers of circulating anti-SARS-CoV-2 IgG in the bloodstream

Coagulation Parameters in Post-Covid-19 Condition in Relation to Various Titers of Anti-SARS-CoV-2 IgG in Blood Plasma

Characteristics of Products of Fibrinogen Origin in the Presence of Anti- SARS-CoV-2 IgG in the Bloodstream

Contact Info

Product

Resources

About