Effect of imiquimod on partial-thickness burns

PURPOSE:To revise and systematize scientific knowledge of the experimental model for cutaneous burns in rats. METHODS:A bibliographical review from 2008 up to January 2011 in PubMed, EMBASE and LILACS was undertaken. Were used the keywords: animal models, burns and rats. 221 studies were identified, and 116 were selected. RESULTS:It was found that: 54/86 (62.7%) had third degree burns; 55/73 (75.3%) studied the back; 45/78 (57.6%) used heated water and 27/78 (35.9%) incandescent instruments; 39/78 (50%) studied systemic effects; 22/71 (31%) used ketamine associated with xylazine; 61/64 (95.3%) performed depilation with appropriate equipment; 36/72 (50%) used microscopy; more than 50% did not describe analgesia or antibiotics during the postoperative period; in 42/116 (36.2%) postoperative fluid therapy was performed; and the time interval after the burn, up to the beginning of the results analysis varied from 7s up to four weeks. Legislation issues on burn experiments are discussed. CONCLUSION:The hot water was the main method to induce burns those of third degree on the back, with anesthesia using ketamine and xylazine, after depilation. These were evaluated microscopically, without using analgesia or an antibiotic during the postoperative period. The studies were not very reproducible. CONCLUSÃO: Líquido aquecido foi o principal método para induzir queimadura de terceiro grau no dorso do animal, com anestesia usando quetamina e xilazina, após depilação, avaliados por microscopia, sem uso de analgesia ou antibióticos. Os estudos não são reprodutíveis.Descritores: Modelos Animais. Queimaduras. Pele. Revisão. Ratos.Mitsunaga Jr JK et al.

“…The formula used to calculate the areas was as follows: [TBSA (cm 2 ) = 9.1BW 2/3 ] where 9.1 is a fixed attributed value and "BW" represents the weight of the animal in grams 21 .…”

Section: Size and Thickness Of Burnmentioning

confidence: 99%

Rat an experimental model for burns: A systematic review

Mitsunaga

Gragnani

Ramos³

et al. 2012

“…Immunohistochemical assessment, in this study, was carried out quantitatively using the software developed specifically for morphometric study already used in other similar works [19][20] .…”

Section: Discussionmentioning

confidence: 99%

“…The dorsal skin was surgically prepared with successive applications of 2% chlorhexidine scrub. A previously described model [19][20] …”

Section: Burnsmentioning

confidence: 99%

Oil mixes omega 9, 6 and 3, enriched with seaweed, promoted reduction of thermal burned modulating NF-kB and Ki-67

Campelo

Brito³

et al. 2015

PURPOSE:To examine the effects of the oil mixes (ω-9, ω-6 and ω-3) in rats subjected to thermal burn. It was also aimed to assess whether the sources of ω3 would interfere with the effect of such mixes on the thermal injury. METHODS:Thirty-six rats distributed into five groups: burned + water, burned + isolipid mix, burned + oil mix 1 (ALA), burned + oil mix 2 (ALA + EPA + DHA of fish) and burned + oil mix 3 (ALA + DHA from seaweed). The thermal injury was involving total thickness of skin. After the burns animals received the oil mixes for seven days. The lesions were evaluated by immunohistochemistry.RESULTS: Animals receiving mix 3 showed a smaller extension of the thermal injury as compared to those that were supplemented with other oils mixes. Expression of Ki-67 in the receiving Mix 3 increased as compared to all the other groups. Animals supplemented with mix 3 were able to inhibit NF-κB in injured tissue. CONCLUSION:Rats received oil mix in which the source of ω3 (ALA+DHA of seaweed) showed inhibition of NF-κB, increase in cell proliferation, and reduction the extension of thermal lesion.Key words: Burn. Fatty Acids. Seaweed. Docosahexenoic Acid. alpha-Linolenic Acid. Rats.Oil mixes omega 9, 6 and 3, enriched with seaweed, promoted reduction of thermal burned modulating

“…Microscopic digital images were obtained as previously described 28 . The points of greatest thickness were located by scanning the sections at a magnification of x100, with …”

Section: Quantitative Analysis Of Capsule Thicknessmentioning

confidence: 99%

“…The involving skin, subjacent tissues, capsules and implants were removed en bloc and preserved for later qualitativequantitative analysis of histologic samples sectioned 5 to 7µm and stained with hematoxylin-eosin (H&E) and histochemical Sirius Red dye (SR) saturated with picric acid as described elsewhere [27][28][29][30] .…”

Section: Microscopic Assessmentmentioning

confidence: 99%

Effect of propranolol on capsular reaction around silicone implants in guinea pigs

Mesquita

Guimarães²,

Filho³

et al. 2015

PURPOSE:To evaluate the effect of propranolol on capsular architecture around silicone implants by measuring the inflammation, capsular thickness, and collagen fiber density, using a guinea pig experimental model. METHODS:Thirty six adult male guinea pigs randomly divided into two groups (n=18) were used. Each one received a silicone implant with textured-surface. The capsular tissue around implants from untreated or treated animals with the beta-adrenoceptor antagonist propranolol (10 mg/kg, dissolved in daily water) were analyzed for inflammation by histological scoring, capsular thickness by computerized histometry, and collagen fibers type I and Type III density by picrosirius polarization at different time points (7, 14 or 21 days after silicone implantation). RESULTS:Propranolol treatment reduced inflammation and impaired capsular thickness and delayed collagen maturation around the textured implant. CONCLUSION:Propranolol reduces the risk of developing capsular contracture around silicone implants with textured surface.