Effect of Immobilization in Alginate on the Stability of Freeze-Dried Bifidobacterium longum

Survival of freeze-dried Lactobacillus acidophilus immobilized in .KAPPA.-carrageenan gel.

“…It was found that immobilized cells possessed better resistance to freezing and freeze-drying compared with free cells. Results obtained were in agreement with those of Champagne et al (1992Champagne et al ( , 1996a and Morin et al (1992), who immobilized Lactococcus lactis with calcium alginate. On the other hand, Bekatorou et al (2001) found that survival of Saccharomyces cerevisiae under freeze-drying could be improved by using cell immobilization on gluten pellets.…”

“…This might also result in a decrease in culture survival during the subsequent storage (Bozoglu et al, 1987;Brennan et al, 1986). Cell immobilization has been examined extensively in the freeze-drying and storage thereafter of lactic acid bacteria, and significant improvement in survival could be obtained and cell concentration of dried samples could be raised (Champagne et al, , 1996aKim et al, 1988;Maitrot et al, 1997;Yoon et al, 1995). Damage to cells caused by low-temperature and freezing might also be reduced by the application of cell immobilization Kearney et al, 1990).…”

Enhancement of freezing-resistance of Lactobacillus rhamnosus by the application of cell immobilization

“…After defrosting, the samples were cultured on MRS agar and the standard plate count method (SPC method) was used to determine the viable cell number (Speck, 1984). For the determination of viable lactic acid bacteria number, Ca-alginate entrapped samples were dissolved in 4% EDTA (ethylenediaminetetraacetic acid) solution (Champagne et al, 1993(Champagne et al, , 1996Kearney et al, 1990), and physiological saline was used for the dissolution of k-carrageenan gel beads (King and Zall, 1983b).…”

Immobilized lactic acid bacteria for application as dairy starters and probiotic preparations

“…Many immobilization techniques for lactic acid bacteria have been examined, but no cell loading above 5ϫ10 11 cfu/g gels has been reported (Champagne et al, 1994;Lamboley et al, 2003). Cell immobilization has been examined extensively in the freeze-drying and storage thereafter of lactic acid bacteria, and significant improvement in survival could be obtained and cell concentration of dried samples could be raised (Champagne et al, 1996;Kornacki and Gabis, 1990;Maitrot et al, 1997;Tsen et al, 2002). Damage to cells caused by freezing might also be reduced by immobilization .…”