Effect of Immune Serum and Its Immunoglobulin Fractions on Hamsters Challenged with Treponema pallidum ssp. pertenue

Azadegan, A A; Schell, Ronald F.; Steiner, Bret; Coe, John E.; Chan, John

doi:10.1093/infdis/153.6.1007

Cited by 12 publications

(10 citation statements)

References 15 publications

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“…A strong correlation has been made between the development of infection-derived immunity in rabbits and the appearance of bactericidal antibodies (6,30). Passive immunization with infection-derived immune serum confers partial to complete protective immunity in experimental animals (1,2,5,38,44,45,50). The target(s) of the bactericidal antibodies has not been identified, although the presumption has been that such a target(s) is on the surface of the spirochete.…”

mentioning

confidence: 99%

A Monoclonal Antibody That Conveys In Vitro Killing and Partial Protection in Experimental Syphilis Binds a Phosphorylcholine Surface Epitope ofTreponema pallidum

Blanco

Champion

Dooley³

et al. 2005

Infect Immun

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Immunization with purified Treponema pallidum outer membrane vesicles (OMV) has previously resulted in high-titer complement-dependent serum bactericidal activity. In this study, OMV immunization resulted in the isolation of a monoclonal antibody, M131, with complement-dependent killing activity. Passive immunization of rabbits with M131 administered intravenously conferred significant immunity demonstrated by the failure of syphilitic lesions to appear at 29% of intradermal challenge sites (7/24) and a mean delay of approximately 8 days to lesion appearance at the remaining sites (17/24). M131 not only bound to OMV and to the surfaces of intact motile T. pallidum cells but also bound to organisms whose outer membranes were removed, indicating both surface and subsurface locations for the killing target. This target was determined to be a T. pallidum lipid. Lipid extracted from T. pallidum and made into liposomes bound M131. Reverse-phase high-pressure liquid chromatography separation and fraction collection mass spectrometry (LC-MS؉) of T. pallidum lipid showed that the target of M131 was phosphorylcholine. M131 binding required both liposome formation and a critical concentration of phospholipid containing phosphorylcholine, suggesting that the epitope has both a conformational and a compositional requirement. M131 did not react with red blood cells, which have phosphorylcholine-containing lipids in their exterior membrane leaflets, or with Venereal Disease Research Laboratory antigen that also contains phosphorylcholine, further indicating the specificity of M131. This is the first physical demonstration of an antigen on the T. pallidum surface and indication that such a surface antigen can be a target of immunity.

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confidence: 99%

A Monoclonal Antibody That Conveys In Vitro Killing and Partial Protection in Experimental Syphilis Binds a Phosphorylcholine Surface Epitope ofTreponema pallidum

Blanco

Champion

Dooley³

et al. 2005

Infect Immun

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“…endemicum (4) or T. pallidum subsp. pertenue (5). Whether the apparently conflicting results are pathogen or host related is not known.…”

Section: Discussionmentioning

confidence: 99%

“…endemicum (4) or T. palidum subsp. pertenue (5) provided full protection to normal syngeneic animals against a challenge infection with the homologous strains of treponemes. In the latter report (5), it has been demonstrated that the immunoglobulin fraction IgG2 exerts better treponemicidal activity in vitro than the IgGl fraction does.…”

mentioning

confidence: 98%

“…pertenue (5) provided full protection to normal syngeneic animals against a challenge infection with the homologous strains of treponemes. In the latter report (5), it has been demonstrated that the immunoglobulin fraction IgG2 exerts better treponemicidal activity in vitro than the IgGl fraction does. The paradoxical situation is that while the immune sera of hamsters are fully effective in adoptive transfer experiments, they are unable to eliminate the pathogen from the donors of immune sera.…”

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confidence: 98%

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Effect of passive immunization with purified specific or cross-reacting immunoglobulin G antibodies against Treponema pallidum on the course of infection in guinea pigs

1992

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Whole immune serum or highly purified immunoglobulin G (IgG) antibodies to Treponema paUidum exhaustively adsorbed with three strains of nonpathogenic treponemes (TPI-IgG) were used for passive immunization of inbred strain 2 guinea pigs before and after intradermal challenge with 3.4 x 107 virulent T. pallidum Nichols organisms. Before challenge, control animals received a similarly purified IgG fraction containing either a cocktail of antibodies against three nonpathogenic treponemes (NPTI-IgG) or IgG prepared from normal guinea pig serum (NGPS-IgG). The purified fractions contained both IgGl and IgG2 isotypes. The antibody levels (detected by fluorescent treponemal antibody test and enzyme-linked immunosorbent assay) and molecular specificities (immunoblot) of sera obtained from recipient animals before infection reflected those of the purified fractions used for immunization. Three protocols of passive immunization were used. Whole immune serum containing specific and cross-reacting antibodies afforded better protection than TPI-IgG even though asymptomatic animals were not fully protected. A single intradermal injection (0.1 ml) of TPI-IgG or NPTI-IgG into one hind leg 22 h before infection at the same site provided relatively higher protection than multiple intravenous injections (total, 15 ml) of the respective individual preparations. Since purified NGPS-IgG injected in the same animals, into the opposite hind leg, failed to protect against the challenging infection, it is reasonable to assume that specific and cross-reacting antitreponemal antibodies of the IgGl subclass, which in guinea pigs are homocytotropic, play a relevant role in local protection.Natural and experimental infections with Treponema pal-* Corresponding author. t This article is dedicated to the memory of Thomas

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“…Schell and coworkers contributed significantly to the exploration of the immune responses and the protective role of antibodies and immune cells in experimental yaws in hamsters (1,2,(30)(31)(32).…”

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confidence: 99%

Treponema pallidumsubsp.pertenueDisplays Pathogenic Properties Different from Those ofT. pallidumsubsp.pallidum

Wicher

Abbruscato

et al. 2000

Infect Immun

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The present study described the susceptibility of C4D guinea pigs to cutaneous infection with Treponema pallidum subsp. pertenue Haiti B strain. The general manifestations of the disease in adults and neonates differ, to a certain degree, from those induced by T. pallidum subsp. pallidum Nichols strain. Noticeable differences between the infections were reflected in the character of the skin lesions, their onset and persistence, and the kinetics of the humoral response. The incidence and dissemination of cutaneous yaws lesions in very young guinea pigs were remarkably different from the low frequency observed in a similar age group of syphilis infection, 100 versus 17%, respectively. Moreover, as opposed to T. pallidum subsp. pallidum, T. pallidum subsp. pertenue does not cross the placenta. Offspring born to yaws-infected mothers did not produce immunoglobulin M antibodies and their organs, examined by PCR and rabbit infectivity test (RIT), were all negative. Examination of a large number of tissues and organs in adult, neonate, and maternal yaws by PCR and RIT clearly demonstrated that, unlike syphilis, there was a low incidence and short persistence of the yaws pathogen in internal organs. These findings stress the dermotropic rather than the organotropic character of yaws and provide further evidence of distinctive biological and pathological differences between yaws and venereal syphilis.

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Effect of Immune Serum and Its Immunoglobulin Fractions on Hamsters Challenged with Treponema pallidum ssp. pertenue

Cited by 12 publications

References 15 publications

A Monoclonal Antibody That Conveys In Vitro Killing and Partial Protection in Experimental Syphilis Binds a Phosphorylcholine Surface Epitope ofTreponema pallidum

A Monoclonal Antibody That Conveys In Vitro Killing and Partial Protection in Experimental Syphilis Binds a Phosphorylcholine Surface Epitope ofTreponema pallidum

Effect of passive immunization with purified specific or cross-reacting immunoglobulin G antibodies against Treponema pallidum on the course of infection in guinea pigs

Treponema pallidumsubsp.pertenueDisplays Pathogenic Properties Different from Those ofT. pallidumsubsp.pallidum

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