Effect of Immunization on Migration of Schistosoma Mansoni through Lungs *

Minard, P; Dean, David A.; Vannier, Wilton E.; Murrell, K. D.

doi:10.4269/ajtmh.1978.27.87

Cited by 31 publications

(22 citation statements)

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“…The irradiated infections have been studied extensively. Thus optimally irradiated infections are known to die at the lung stage (Minard et al 1978, Bickle, Dobinson & James (1979 after an extended period of survivial (Mastin, Bickle & Wilson 1983). The patterns of antibodies produced against larval surface antigens and epitopes have been studied (Simpson et al 1983, Omer-Ali et al 1988) and insights gained into the nature of the immunity such as its schistosome species specificity (Bickle et al 1986, Cheever et af.…”

Section: Discussionmentioning

confidence: 99%

Induction of immunity against Schistosoma mansoni by drug (Roll‐3128)‐terminated infections: analysis of surface antigen recognition

Bickle

Sacko

Vignali

1990

Parasite Immunology

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As with 20 krad-irradiated infections in mice, the present study shows that the immunity induced by Ro11-3128 termination of unattenuated infections at the skin stage is species specific, not operating against S. japonicum. Treatment with the drug Ro15-5458, also effective at the skin stage, however, resulted in significantly lower levels of resistance than Ro11-3128. Sera from mice immunized by infection plus Ro11-3128 treatment on days 1 or 2 (Ro11S) coprecipitated essentially the same pattern of 125I-labelled surface antigens as the 20 krad vaccine serum (VMS), viz. Mr 38,000, 32,000, 23,000 and 15,000. However, recognition by Ro11S was markedly stronger. Sera from the infected and Ro15-5458-treated mice (Ro15S) failed to recognize the Mr 23,000 antigen and produced a weaker response than Ro11S or VMS against the Mr 38,000 or 32,000 antigens but a comparable response to VMS against the Mr 15,000 antigen. Ro11S and VMS also recognized the Mr 16,000 surface antigen seen by Western blotting but its recognition by Ro15S was weaker. Compared with sera from animals treated at the skin stage, sera from animals treated at the lung stage (day + 6) showed weaker recognition of the Mr 32,000 and 15,000 antigens and no recognition of the Mr 23,000 antigen. In contrast, sera from mice treated at 15 days recognized both the Mr 32,000 and 23,000 antigens but not the Mr 15,000 antigen. Mice treated at these times show progressively less immunity than at the skin stage. Infected but untreated animals only showed significant recognition of the Mr 32,000 antigen. Thus compared with infections treated with Ro11-3128 on days 1 or 2, treatment at later times or with the drug Ro15-5458 resulted in selective and differential absence or diminution of response against either the Mr 38,000, 32,000, 23,000, 16,000 or 15,000 antigens. In vitro, Ro11-3128, in contrast to Ro15-5458, caused multiple vesicle formation at the surface of skin stage schistosomula but this was progressively less pronounced with lung and liver stage worms. The vesicles were shown to express surface membrane antigens but were apparently not derived from the existing outer leaflet of the surface membrane. It is suggested that this altered antigen expression might explain the optimum immunity induced.

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Section: Discussionmentioning

confidence: 99%

Induction of immunity against Schistosoma mansoni by drug (Roll‐3128)‐terminated infections: analysis of surface antigen recognition

Bickle

Sacko

Vignali

1990

Parasite Immunology

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“…One of the best hopes for controlling this disease is by immunization. Vaccination of experimental animals by exposure to the infective stage of the parasite (cercaria) previously attenuated by high-dose irradiation has been shown to result in partial immunity to subsequent infection (Minard et al 1978a) with minimal vaccine-related pathology (Minard et al 1978b). Indeed, this method of vaccination has already been used successfully to immunize sheep (Taylor et al 1976) and cattle (Bushara et al 1978) against schistosomiasis.…”

Section: Introductionmentioning

confidence: 99%

Mechanisms of protective immunity against Schistosoma mansoni infection in mice vaccinated with irradiated cercariae III. Identification of a mouse strain, P/N, that fails to respond to vaccination

James

Sher

1983

Parasite Immunology

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Eleven strains of inbred mice were examined for their ability to develop resistance to challenge Schistosoma mansoni infection as a result of previous exposure to homologous cercariae that had been attenuated by high-dose irradiation. Two strains, C57B1/6J and BALB/c, demonstrated consistently high levels of vaccine-induced immunity (means of 64% and 58% resistance, respectively, when compared to control groups of the same strain) and were designated as 'high responder' strains to vaccination. Six other strains fell into an intermediate category, demonstrating moderate, yet statistically significant, levels of immunity resulting from vaccination (means of 30-50% resistance). Only one of the strains examined consistently failed to respond to vaccination by the development of significant levels of immunity to challenge infection. Animals of the P/N strain demonstrated a mean of only 15% resistance to challenge in five experiments and have been classified as 'low responders' to vaccination. P/N mice have previously been characterized as deficient in their ability to mount delayed hypersensitivity reactions, produce lymphokine and display macrophage activation for cytolysis of extracellular and intracellular targets in other experimental systems, suggesting that these immune responses may be critical to the establishment of vaccine-induced resistance to S. mansoni infection. The availability of high and low responder mouse strains should facilitate a genetic approach to characterization of the immune effector mechanism(s) of vaccine-induced resistance to S. mansoni infection.

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“…It was soon established that for resistance induced by a single exposure of mice the dose of radiation had a significant effect. Both low doses of radiation, allowing parasite survival through to stunted adult worms, and very high doses, arresting the larvae in the skin, were poorly effective whereas significantly higher protection could be induced with optimal doses (56 krad (Minard et al 1978 a) or 20 krad (Bickle et al 1979 c)). In both cases the effects of these optimal doses of radiation on the larvae were similar, resulting in a slight delay in the skin but eventual migration to and death in the lungs (Minard et al 1978 b;Bickle et al 1979 b;Mastin et al 1983 ;Mangold and Dean, 1984).…”

Section: R R a D I A T E D V A C C I N E S -E S T A B L I S H M E Nmentioning

confidence: 99%

“…This is consistent with the demonstration by CauladaBenedetti et al (1991) that following repeated (3r) exposure Th2 cytokines are upregulated at the expense of Th1 cytokines. Repeat vaccination also leads to somewhat enhanced protection (Minard et al 1978 a ;Anderson et al 1999 ;Jankovic et al 1999) and to boosting of antibody titres (Delgado and McLaren, 1990 ;Caulada-Benedetti et al 1991 ;Anderson et al 1999 ;Jankovic et al 1999). This boosting of the antibody response explains the increased protection since both occur in IFN-c KO but not in B-cell-deficient mice (Anderson et al 1999 ;Jankovic et al 1999).…”

Section: Antibody-mediated Immunitymentioning

confidence: 99%

Radiation-attenuated schistosome vaccination – a brief historical perspective

Bickle

2009

Parasitology

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The high level of protection which can be induced by vaccination of a range of hosts, from rodents to primates, with live radiation-attenuated schistosome larvae offers great promise for development of a human schistosome vaccine. Studies of the irradiated vaccine models benefitted from significant funding during the 1970-90s and much was learned concerning the inducers, targets and mechanisms of immunity. Less progress was made in definition of the protective antigens involved. The application of new techniques for identifying membrane and secreted antigens has recently provided new vaccine candidates and a new impetus for schistosome vaccine development. This article is intended as an overview of some of the main lessons learned from the studies of the irradiated vaccines as a backdrop to renewed interest in schistosome vaccine development.

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Effect of Immunization on Migration of Schistosoma Mansoni through Lungs *

Cited by 31 publications

References 0 publications

Induction of immunity against Schistosoma mansoni by drug (Roll‐3128)‐terminated infections: analysis of surface antigen recognition

Induction of immunity against Schistosoma mansoni by drug (Roll‐3128)‐terminated infections: analysis of surface antigen recognition

Mechanisms of protective immunity against Schistosoma mansoni infection in mice vaccinated with irradiated cercariae III. Identification of a mouse strain, P/N, that fails to respond to vaccination

Radiation-attenuated schistosome vaccination – a brief historical perspective

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