Effect of Immunostimulation by Detoxified E. coli Lipopolysaccharide Combined with Inactivated Propionibacterium granulosum Cells on Porcine Immunity

Abstract

ABSTRACT. The aim of this experiment was to evaluate the immunomodulating activities of inactivated Propionibacterium granulosum cell walls and E. coli lipopolysaccharide (PG/LPS) on porcine immunity. Piglets were intramuscularly administered PG/LPS (1 ml/10 kg body weight) once or twice. The function of natural killer cells, lymphocytes and neutrophils and the adjuvant effect on antibody induction by attenuated classical swine fever virus (CSFV) and inactivated Mycoplasma hyopneumoniae vaccination were evaluated. The results showed that the cytotoxicity of natural killer cells and proliferation of lymphocytes in response to mitogen stimulation were significantly enhanced (P<0.05) in those pigs receiving PG/LPS injection compared with the controls. However, there was no significant effect on the phagocytic activity of neutrophils (P>0.05). PG/LPS also displayed adjuvant effects with CSFV and Mycoplasma hyopneumoniae vaccines. Moreover, pigs receiving two injections of PG/LPS showed a 20.8% growth enhancement compared with untreated pigs. Thus, PG/LPS caused positive immunoregulation of porcine innate immune system effectors, non-specific activation of lymphocytes and antibody production.

Dairy Propionibacteria: Less Conventional Probiotics to Improve the Human and Animal Health

“…The usefulness of the prophylactic application of this strain, against porcine microbial infections was tested in swine finding out that propionibacteria application caused positive immunoregulation of porcine innate immune system effectors, non-specific activation of lymphocytes and antibody production that resulted in milder clinical symptoms, faster recovery and a larger body weight gain [157,158].…”

Therapeutic Effects of Propionibacterium acnes and Lipopolysaccharide from Escherichia coli in Cats with Feline Panleukopenia

“…In pigs, the administration of lipopolysaccharide (LPS) and Propionibacterium granulosum along with a vaccine resulted in higher primary humoral responses than those in pigs receiving the vaccine alone [ 13 ]. Another study demonstrated that the combination of lipopolysaccharide (LPS) and Propionibacterium granulosum significantly enhanced the cytotoxicity of natural killer cells and lymphocyte proliferation in response to mitogen stimulation ( p < 0.05) in pigs, indicating positive immunoregulation of the porcine innate immune system, non-specific activation of lymphocytes, and antibody production [ 14 ].…”

Utjecaj imunomodulatora virusnog ili bakterijskog podrijetla i cjepiva protiv bolesti Aujeszkoga na udio B limfocita periferne krvi na tovljenu prasad

“…Pigs that received PG/LPS had increased growth rate, and the PG/ LPS combination proved to possess good adjuvant effects by enhancing the immune response to a vaccine prepared with the inactivated microorganism Mycoplasma hyopneumoniae and a vaccine with attenuated classical swine fever virus. It could be administered concomitantly in the field with vaccines, thus improving both non-specific and specific immune responses to enhance porcine resistance to infection (Lo et al, 2009).…”