Effect of imported fire ant extract on the degradation of mountain cedar pollen allergen

Meier, Eric A.; Whisman, Bonnie A.; Rathkopf, Melinda M.

doi:10.1016/s1081-1206(10)61036-8

Cited by 13 publications

(7 citation statements)

References 11 publications

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“…This way of immunotherapy was able to induce the patient's tolerance to the ant. The ant abdomen portion may include many kinds of proteins that are not related to the patient's anaphylaxis, however, the whole body was selected as the best operative possible option, instead of the ant head portion alone, considering that whole-body preparation had been previously fruitful reported in literature [15]. In addition, fire ant whole-body extract, unlike other Hymenoptera whole-body extracts, retains its allergenic activity and is an adequate material for use in diagnostic testing and immunotherapy.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of subcutaneous immunotherapy achieved with a whole-body extract of Pseudomyrmex ant. A case reported in a young woman, in Argentina

Rodriguez¹,

Irañeta²,

Olgiati³

et al. 2018

Integr Mol Med

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The aim of this work is to report for the first time a patient case with anaphylaxis by Pseudomyrmex acanthobius /favidulus ant sting, prepare an extract with the wholeant-body, to study their biochemical and immunological properties and to validate the efficacy of the subcutaneous immunotherapy with this non-commercial extract. An argentine woman patient, 19 years old, with background of allergic rhinitis and bronchial asthma in the childhood, suffered repeated episodes of anaphylaxis by non-identified insect's stings and previous immunotherapies treatment. The entomologic analysis revealed that the aggressor insect was an ant belonging to Pseudomyrmex genera and acanthobius or favidulus species. High serum total levels of IgE (202 UI/ml) were measured by ELISA and a positive 6-mm wheal and flare reaction (extract dilution 1/100000) was revealed by in vivo intradermal test. A sample of the extract proteins fraction was analysed by SDS-PAGE. The silver stained protein bands ranged since 20 to 220 kDa. The patient serum immune-recognized allergenic extract proteins at approximately 160, 90, and a double band at 42/46 kDa prior to desensitization treatment by electro-blotting. Interestingly, post-specific-subcutaneous-immunotherapy the mentioned double band almost disappeared. After a 6-years-treatment, the total IgE serum values strongly decreased and the specific intradermal test was negative up to 1/10 extract dilution. The tolerance to the treatment was good. Altogether, our findings revealed that the immunotherapy performed with the whole-body-Pseudomyrmex ant allergenic extract was highly effective and the IgE specific-double protein allergenic extract bands (42/46 kDa) that disappeared after immunotherapy were responsible for the anaphylactic shock.

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Section: Discussionmentioning

confidence: 99%

Efficacy of subcutaneous immunotherapy achieved with a whole-body extract of Pseudomyrmex ant. A case reported in a young woman, in Argentina

Rodriguez¹,

Irañeta²,

Olgiati³

et al. 2018

Integr Mol Med

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“…It is well known that the stability of allergen extracts may be altered by several factors, including proteolytic effects [2–4, 7, 27–29]. To avoid them, it is generally recommended not only to mix different allergen extracts for hyposensitization therapy [3] but also to inject them into different arms.…”

Section: Discussionmentioning

confidence: 99%

“…However, in practice, many researchers tend to mix allergen extracts from different biologic materials in their immunotherapy studies, even though their composition is only partially known, to simplify the administration. Otherwise, allergenic potency can diminish during storage, after dilution or by mixture with allergen extracts containing proteases [1–4], and the presence of these enzymes in allergen extracts has been well documented [5–7]. Unfortunately, the loss in potency still affects the results of proper allergy diagnosis and immunotherapy.…”

Section: Introductionmentioning

confidence: 99%

MALDI‐TOF MS analysis of labile Lolium perenne major allergens in mixes

Irañeta

Acosta

Durán

et al. 2008

Clin Experimental Allergy

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Lol p 1 and Lol p 5 degradation is responsible for the loss of the biological activity of L. perenne pollen extract when co-incubated with protease-rich fungal and cockroach extracts in the same vial for months in the absence of glycerol as a preserving agent. The integrity of these major allergens must be preserved to increase the vaccine stability and to assure efficacy when mixes are used for immunotherapy.

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“…According to the ESPRINT-15 questionnaire, the 39 patients evaluated described a significant improvement in quality of life at both 6 and 12 months after starting the administration of the vaccine (Table 4) Until now, it was assumed that this type of mixture was not functional because the enzymatic activity of some allergens compromised the structural integrity of others (12)(13)(14)(15)(16)(17). However, a study by Grier et al showed that immunotherapy based on a combination of allergens from different species is possible (18).…”

Section: Rhinitis Control and Quality Of Life For Patients With Rhinitismentioning

confidence: 99%

A preliminary study to investigate effectiveness of a mixed extract of Dermatophagoides sp. house dust mites and Alternaria sp. mold

El-Qutob

Isabela

Mencia

2021

Eur Ann Allergy Clin Immunol

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Effect of imported fire ant extract on the degradation of mountain cedar pollen allergen

Cited by 13 publications

References 11 publications

Efficacy of subcutaneous immunotherapy achieved with a whole-body extract of Pseudomyrmex ant. A case reported in a young woman, in Argentina

Efficacy of subcutaneous immunotherapy achieved with a whole-body extract of Pseudomyrmex ant. A case reported in a young woman, in Argentina

MALDI‐TOF MS analysis of labile Lolium perenne major allergens in mixes

A preliminary study to investigate effectiveness of a mixed extract of Dermatophagoides sp. house dust mites and Alternaria sp. mold

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