Effect of Imput Multiplicity and Tissue Cell Concentration on Growth of Rift Valley Fever Virus

Orlando, Michael D.; DeLauter, Richard D.; Riley, Jean M.

doi:10.1128/am.15.3.594-596.1967

Appl Microbiol

1967

DOI: 10.1128/am.15.3.594-596.1967

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Effect of Imput Multiplicity and Tissue Cell Concentration on Growth of Rift Valley Fever Virus

Michael D. Orlando¹,

Richard D. DeLauter²,

Jean M. Riley³

Abstract: The effects of virus input multiplicity and of tissue cell concentration upon the growth of Rift Valley fever virus in L cells (Earle) were determined. The titers obtained in suspension cultures with cells obtained from two separate laboratories were significantly different. With both monolayer culture and suspension culture systems, a virus input multiplicity of 2.5 resulted in the greatest proliferation of virus. Optimal viral yields were obtained in suspension cultures containing 4 × 10 5 … Show more

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1969

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Evaluation of Factors Related to Growth of Rift Valley Fever Virus in Suspended Cell Cultures

Walker¹,

Carter²,

Klein³

et al. 1969

Appl Microbiol

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The effect of several controlled variables on the peak titer and fold increase of Rift Valley fever virus grown in suspension culture on two variants of Earle's L cell, L-DR and L-MA clone 1-1, was studied. No significant amount of cell-associated virus was found at 24 hr, indicating a release of virus soon after its formation. Mild sonic treatment of the virus produced in serum-free medium increased the infective titer about 10×. This difference was not observed with virus produced in medium supplemented with serum. Peak titer was not affected by medium used during the infection period, by multiplicity of inoculum (MOI), or by initial cell concentration within the test range of 10 4 to 2 × 10 6 cell/ml. Cell strain employed influenced titer, because the L-DR cell did not produce virus efficiently at low MOI and low initial cell concentration. The time of peak titer and fold replication was dependent on MOI and initial cell concentration. Differences in virus propagation in monolayer and suspension systems are discussed.

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Evaluation of Factors Related to Growth of Rift Valley Fever Virus in Suspended Cell Cultures

Walker¹,

Carter²,

Klein³

et al. 1969

Appl Microbiol

View full text Add to dashboard Cite

show abstract

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Effect of Imput Multiplicity and Tissue Cell Concentration on Growth of Rift Valley Fever Virus

Cited by 1 publication

References 4 publications

Evaluation of Factors Related to Growth of Rift Valley Fever Virus in Suspended Cell Cultures

Evaluation of Factors Related to Growth of Rift Valley Fever Virus in Suspended Cell Cultures

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