Effect of in ovo zinc injection on the embryonic development, tissue zinc contents, antioxidation, and related gene expressions of broiler breeder eggs

Sun, Xiaoming; Liao, Xiudong; Lü, Lin; Zhang, Liyang; Ma, Qiugang; X, Lin; Luo, Xugang

doi:10.1016/s2095-3119(17)61704-0

Cited by 15 publications

(14 citation statements)

References 36 publications

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“…The primers of DMT1, FPN1, EAAT3, LAT1, B 0 AT1, y + LAT1, y + LAT2, β-actin (housekeeping gene) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene (housekeeping gene) were chosen with Primer Express Software (Applied Biosystems Inc., Foster, CA), and their information was given in Table 1. The expression of target genes was normalized using the geometric mean of β-actin and GAPDH with the 2 −ΔΔCT method as described previously (Livak and Schmittgen, 2001;Sun et al, 2017).…”

Section: Analyses Of Mrna Expression Levels By Real-time Quantitative Pcrmentioning

confidence: 99%

Organic iron absorption by in situ ligated jejunal and ileal loops of broilers1

Lü

Zhang

et al. 2018

Journal of Animal Science

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The objective of this study was to determine the effect of organic and inorganic Fe sources on the Fe absorption and gene expression of Fe and amino acid transporters in the ligated jejunal and ileal segments of broilers. The in situ ligated jejunal and ileal loops from Fe-deficient broiler chicks (28-d-old) were perfused with Fe solutions containing 0, 3.58, or 7.16 mM Fe from one of the following Fe sources: Fe sulfate (FeSO 4 •7H 2 O), the mixtures of FeSO 4 •7H 2 O with either Met or Gly, Fe-Gly chelate, or three Fe-amino acid or protein chelates with weak, moderate or extremely strong chelation strengths (Fe-Met W, Fe-Pro M, or Fe-Pro ES), respectively, for up to 30 min. Iron absorption was increased (P < 0.0001) as the perfused Fe concentrations increased, and no differences (P > 0.07) were detected in the Fe absorption between the jejunum and ileum. Regardless of intestinal segments, Fe absorption was higher (P < 0.006) for Fe-Pro ES and Fe-Pro M than for FeSO 4 •7H 2 O, and for Fe-Pro ES than for Fe-Met W. Glycine but not Met supplementation increased (P < 0.03) the absorption of Fe as FeSO 4 . Regardless of Fe source, Fe addition inhibited (P < 0.05) the mRNA expressions of divalent metal transporter 1 (DMT1) in the jejunum and ileum, but enhanced (P < 0.05) the mRNA expressions of l-type amino transporter 1 (LAT1) and B 0 -type amino acid transporter 1 (B 0 AT1) in the jejunum and ileum. No differences (P > 0.05) among different Fe sources were observed in the mRNA expression levels of Fe and amino acid transporters in both the jejunum and the ileum. The mRNA expression levels of DMT1, ferroportin 1, B 0 AT1, or y + LAT1 were higher (P < 0.0001), but those of excitatory amino acid transporter 3, LAT1, or y + ltype amino transporter 2 were lower (P < 0.04) in the jejunum than in the ileum. The supplementation of inorganic or organic Fe had no effect (P > 0.14) on the protein expression levels of DMT1 and FPN1 in the jejunum and ileum. The above results indicate that organic Fe sources with stronger chelation strengths showed higher Fe absorption in the jejunum and ileum of broiler chicks. Glycine was more effective in facilitating Fe absorption than Met as a ligand. The mRNA expressions of Fe and amino acid transporters in the jejunum were different from those in the ileum. The DMT1, LAT1, and B 0 AT1 might be involved in the Fe absorption in the jejunum or ileum of broilers.

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Section: Analyses Of Mrna Expression Levels By Real-time Quantitative Pcrmentioning

confidence: 99%

Organic iron absorption by in situ ligated jejunal and ileal loops of broilers1

Lü

Zhang

et al. 2018

Journal of Animal Science

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“…Then, a total of 120 fertilized eggs (46.3 ± 0.3 g) were divided into 4 treatment groups of 30 eggs per group. The feather buds began to form on embryonic day (E) 9, and a previous study has demonstrated that in ovo injection at E9 has no effect on the shelling rate ( Sun, et al., 2018 ). Therefore, all in ovo injection solutions were freshly prepared at 9 D of incubation, and the solutions were injected into the egg yolk sac.…”

Section: Methodsmentioning

confidence: 99%

“…The in ovo injection procedure was performed according to the study by Sun et al. (2018) with modifications.…”

Section: Methodsmentioning

confidence: 99%

The Wnt/β-catenin signaling pathway is involved in regulating feather growth of embryonic chicks

et al. 2020

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Avian feathers have robust growth and regeneration capability and serve as a useful model for decoding hair morphogenesis and other developmental studies. However, the molecular signaling involved in regulating the development of feather follicles is unclear. The purpose of this study was to investigate the role of the Wnt/β-catenin pathway in regulating feather morphogenesis in embryonic chicks through in ovo injection of different doses of Dickkopf-1 (DKK1, a specific inhibitor of the target of the Wnt/β-catenin pathway). A total of 120 fertilized embryo eggs were randomly divided into 4 treatments, including a noninjection group (control group) and groups injected with 100 μL of phosphate-buffered saline (PBS)/egg (PBS control group), 100 μL of PBS/egg containing 600-ng DKK1/egg (600-ng DKK1 group), and 100-μL PBS/egg containing 1,200-ng DKK1/egg (1,200-ng DKK1 group). Feathers and skin tissues were sampled on embryonic (E) day 15 and the day of hatching to examine the feather mass, diameter and density of feather follicles, and the protein expression of the Wnt/β-catenin pathway. The results showed that, compared with CON and PBS treatment, the injection of DKK1 into the yolk sac of chick embryos had no significant effect on the hatching rate and embryo weight ( P > 0.05), while it significantly decreased the relative mass of feathers in the whole body ( P < 0.05). The high dose of DKK1 (1,200-ng DKK1/egg) decreased the relative mass of feathers on the back, chest, belly, neck, wings, head, and legs, which was more obvious than that in the 600-ng DKK1 group, which presented a dose-dependent effect. In addition, DKK1 injection significantly downregulated the protein expression levels of β-catenin, transcription factor 4, Cyclin D1, and c-Myc ( P < 0.05). The immunofluorescence result of β-catenin was consistent with the Western blotting assay results. Altogether, these observations suggested that the Wnt/β-catenin signaling pathway is involved in regulating feather follicle development and feather growth during the embryonic development of chicks.

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“…Each gene was independently amplified in triplicate within a single instrument run. relative mRNA expression of target gene was calculated using the 2 −ΔΔCT method (Livak & Schmittgen, ), and data were normalized by β ‐actin and GAPDH as internal controls (Sun et al, ).…”

Section: Methodsmentioning

confidence: 99%

Kinetics of phosphorus absorption and expressions of related transporters in primary cultured duodenal epithelial cells of chick embryos

Cao

Zhang

Liu

et al. 2019

Animal Physiology Nutrition

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Two experiments were conducted to investigate the kinetics of phosphorus (P) absorption and expressions of type IIb sodium-dependent phosphate cotransporter (NaP-IIb), inorganic phosphate transporters 1 and 2 (PiT-1 and PiT-2) in primary cultured duodenal epithelial cells of chick embryos. In experiment 1, the P absorptions across duodenal epithelial cell monolayers at different incubation time points (0, 10, 20, 40, 60, 80, 100 and 120 min) were compared. In experiment 2, the kinetics of P absorption was performed at 40 min after incubation of duodenal epithelial cells with the media containing 0, 0.75, 1.5, 3.0, 6.0, 12.0, 24.0 and 48.0 mmol P/L as KH 2 PO 4 , and the mRNA and protein expression levels of NaP-IIb, PiT-1 and PiT-2 in duodenal epithelial cells with the media containing 0, 6.0 and 48.0 mmol P/L were determined at 87 min after incubation. The results from experiment 1 showed that the P absorption increased linearly (p < .0001) from 0 to 80 min and the fastest increase occurred at 40 min; the asymptotic model was shown to have the best fit degree, and the optimal incubation time for saturable P absorption was determined to be 87 min. The kinetic curves of P absorption from experiment 2 demonstrated that P absorption was a mixed process of a non-saturable diffusion plus a saturable carriermediated transport across the duodenal epithelial cells. The high P concentration (48.0 mmol/L) decreased (p < .05) NaP-IIb and PiT-1 mRNA and protein levels and increased (p < .0001) PiT-2 mRNA level. These results indicated that the P absorption across primary cultured duodenal epithelial cell monolayers of chick embryos was a mixed process of a non-saturable diffusion plus a saturable carrier-mediated transport and could be restricted by reducing the NaP-IIb and PiT-1 expressions while increasing the PiT-2 expression at a high P concentration. K E Y W O R D Sabsorption kinetic, chick embryo, phosphate transporter, phosphorus, primary cultured duodenal epithelial cell

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Effect of in ovo zinc injection on the embryonic development, tissue zinc contents, antioxidation, and related gene expressions of broiler breeder eggs

Cited by 15 publications

References 36 publications

Organic iron absorption by in situ ligated jejunal and ileal loops of broilers1

Organic iron absorption by in situ ligated jejunal and ileal loops of broilers1

The Wnt/β-catenin signaling pathway is involved in regulating feather growth of embryonic chicks

Kinetics of phosphorus absorption and expressions of related transporters in primary cultured duodenal epithelial cells of chick embryos

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