Effect of in vitro and in vivo conditions on development of parthenogenetic rabbit embryos after vitrification

Abstract

ElsevierNaturil Alfonso, C.; Jiménez Trigos, ME.; Vicente Antón, JS.; Marco Jiménez, F. (2015). Effect of in vitro and in vivo conditions on development of parthenogenetic rabbit embryos after vitrification. Cryobiology. 71(1):91-96. doi:10.1016/j.cryobiol.2015.05.003. 1" "Effect of in vitro and in vivo conditions on development of Reproducción. Universidad Politécnica de Valencia, 46022 Valencia, Spain. Valencia, C/Camino de Vera s/n, 46022 Valencia, Spain. 13"Email address: fmarco@dca.upv.es.14" 15"Tel.: +34 96 3879435; fax: +34 96 3877439. and vitrification on rabbit parthenogenetic embryos. Parthenotes were cultured under in 22" vivo and in vitro conditions until day 3 (late morula/early blastocyst), when they were 23" vitrified. Immediately after warming, they were newly cultured under in vivo and in 24"vitro conditions till day 6 (blastocyst stage). Both culture conditions showed similar late 25" morula/early blastocyst (0.39 ± 0.056 vs. 0.46 ± 0.043, for in vivo and in vitro, 26" respectively) and blastocyst rates (0.12 ± 0.068 vs. 0.13 ± 0.070, for in vivo and in vitro, 27" respectively). However, no parthenote was recovered when a combination of culture 28" conditions was performed. To our best knowledge, this is the first demonstration of the the importance of culture conditions on the morphology of parthenote embryos. 32"Therefore, we have described that special attention should be paid on culture conditions 33" to generate parthenote embryos, with a view to their subsequent use, for example in 34"embryonic stem cell production. 35"36"