Effect of in vivo growth conditions upon expression of surface protein antigens in Enterococcus faecalis

Lambert, P

doi:10.1016/0928-8244(90)90052-t

Cited by 3 publications

(4 citation statements)

References 6 publications

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“…Several investigators have demonstrated that serum may modulate bacterial surface antigen expression (Guzman et al, 1989(Guzman et al, , 1991Lambert et al, 1990;Lowe et al, 1995). Guzman et al (1989Guzman et al ( , 1991 suggested that adherence of E. faecalis to eukaryotic cells could be mediated by carbohydrate residues present on the bacterial cell surface.…”

Section: Surface Adhesinsmentioning

confidence: 99%

Virulence Factors ofEnterococcus faecalis: Relationship to Endodontic Disease

Kayaoğlu

Ørstavik²

2004

Critical Reviews in Oral Biology & Medicine

403

247

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Enterococcus faecalis is a micro-organism that can survive extreme challenges. Its pathogenicity ranges from life-threatening diseases in compromised individuals to less severe conditions, such as infection of obturated root canals with chronic apical periodontitis. In the latter situation, the infecting organisms are partly shielded from the defense mechanisms of the body. In this article, we review the virulence factors of E. faecalis that may be related to endodontic infection and the periradicular inflammatory response. The most-cited virulence factors are aggregation substance, surface adhesins, sex pheromones, lipoteichoic acid, extracellular superoxide production, the lytic enzymes gelatinase and hyaluronidase, and the toxin cytolysin. Each of them may be associated with various stages of an endodontic infection as well as with periapical inflammation. While some products of the bacterium may be directly linked to damage of the periradicular tissues, a large part of the tissue damage is probably mediated by the host response to the bacterium and its products.

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Section: Surface Adhesinsmentioning

confidence: 99%

Virulence Factors ofEnterococcus faecalis: Relationship to Endodontic Disease

Kayaoğlu

Ørstavik²

2004

Critical Reviews in Oral Biology & Medicine

403

247

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“…E. faecalis produces a number of surface protein antigens which can be exploited in the serodiagnosis of E. faecalis endocarditis (1,2,5,20). We have shown previously that three cell wall proteins with molecular sizes of 73, 40, and 37 kDa are prominent antigens which are expressed strongly following growth in serum.…”

mentioning

confidence: 99%

Cloning of an Enterococcus faecalis endocarditis antigen: homology with adhesins from some oral streptococci

1995

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Serum from a patient with Enterococcus faecalis endocarditis was used to identify the gene efaA cloned in Lambda ZapII in Escherichia coli. Nucleotide sequence analysis revealed a 924-bp open reading frame encoding a protein with a predicted molecular weight of 34,768. The amino acid sequence of EfaA shows 55 to 60% homology to a group of streptococcal proteins, FimA from Streptococcus parasanguis, SsaB from Streptococcus sanguis, ScaA from Streptococcus gordonii, and PsaA from Streptococcus pneumoniae. Members of this group have been shown to be adhesins, and we hypothesize that EfaA may function as an adhesin in endocarditis.

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“…Bacterial cells were grown in vivo on silastic (Dow Corning Corp., Midland, Mich.) implant de-vices, as described by Ward et al (28), which were sterilized with ethylene oxide and seeded by immersing them in a S. epidermidis culture in TSB medium overnight. They were washed by dipping them in sterile phosphate-buffered saline (PBS) and transferred individually to gas-sterilized acrylic implant chambers equipped with 0.45-,um-pore-size filters on both ends to prevent the migration of phagocytes into the chamber (21,28). The chambers were filled with ca.…”

Section: Methodsmentioning

confidence: 99%

“…3 ml of PBS to facilitate growth. A mid-line incision was made in two anesthetized pigs and four precolonized chambers were sewn to the peritoneal wall of each pig and left in situ for 21 days. The pigs were then euthanized by a barbiturate overdose, and the devices were aseptically removed from the chambers and immediately placed in 50 ml of sterile PBS.…”

Section: Methodsmentioning

confidence: 99%

Effect of growth conditions on expression and antigenicity of Staphylococcus epidermidis RP62A cell envelope proteins

et al. 1993

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Staphylococcus epidermidis RP62A (ATCC 35984) was grown in tryptic soy broth (TSB), iron-depleted TSB (TSB-Fe), iron-reconstituted TSB-Fe (TSB+Fe), a chemically defined medium, and fetal calf serum (FCS) and on silastic disks in chambers that were sutured to the pig peritoneal wall. Bacterial cell wall proteins were extracted by digestion with recombinant lysostaphin, separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and detected by silver staining. Cell wall proteins from TSB-, chemically defined medium-, or FCS-grown cells had a complex profile of greater than 25 protein bands spanning the full molecular mass range. By contrast, a digest obtained from in vivo-grown cells had only five major proteins of 40 kDa or greater. Proteins of 130 and 106 kDa were present in the cell envelopes of TSB-Feand in vivo-grown cells but not in those grown in TSB or TSB+Fe. A 43-kDa protein expressed by in vitro-grown cells and 52and 96-kDa proteins expressed by in vivo-grown cells reacted with antisera from pigs with the chamber implants and from catheterized, paracatheter-inoculated pigs but not with hyperimmune sera from pigs immunized with TSB-grown cells. The data indicate that S. epidermidis, growing under in vivo conditions, expresses antigens distinct from those that are grown in vitro.

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Effect of in vivo growth conditions upon expression of surface protein antigens in Enterococcus faecalis

Cited by 3 publications

References 6 publications

Virulence Factors ofEnterococcus faecalis: Relationship to Endodontic Disease

Virulence Factors ofEnterococcus faecalis: Relationship to Endodontic Disease

Cloning of an Enterococcus faecalis endocarditis antigen: homology with adhesins from some oral streptococci

Effect of growth conditions on expression and antigenicity of Staphylococcus epidermidis RP62A cell envelope proteins

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