Effect of including growth factors and antioxidants in maturation medium used for in vitro culture of buffalo oocytes recovered in vivo

Singhal, Sameer; Prasad, Shiv; Singh, Balraj; Prasad, J.K.; Gupta, H. P.

doi:10.1016/j.anireprosci.2008.05.078

Cited by 28 publications

(34 citation statements)

References 29 publications

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“…In studies without supplementary antioxidant substances, maturated and cultured embryos from different animal species and on different media reaching the blastocyst stage were 7.2% for bovines in TCM-199 and 6% in SOF, while in pigs it was 23.3% in SOF (2,10,29). As shown in Table 1, our cleavage rates were 47.9% (Group AI-SOF), 50.1% (Group AII-CR1aa), 52.2% (Group BI-SOF), and 49.4% (Group BII-CR1aa).…”

Section: Discussionmentioning

confidence: 99%

“…There was, however, no statistically significant difference between the rates of these groups. Singhal et al (29) reported the cleavage rate of buffalo oocytes as 60.7% when they were supplemented with 50 µM cysteamine. Oyamada and Fukui (10) investigated the effect of epidermal growth factor and : Significant difference between values in the same column without a common letter (P < 0.05).…”

Section: Discussionmentioning

confidence: 99%

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Vitrification of in vitro-produced bovine embryos matured inmodified TCM-199 medium

Sandal¹,

Özdaş²

2015

Turk J Vet Anim Sci

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In vitro-produced bovine embryos matured in modified Tissue Culture Medium 199 were vitrified at the 7th and 8th days of culture, and development at 48 h after warming was recorded. Ovaries were obtained from a local abattoir, and the oocytes were recovered by slicing method and divided into two main groups. The first group included cysteamine in the TCM-199 (Group A) and the second did not (Group B). They were incubated for 24 h at 38.8 °C in an atmosphere of 5% CO 2 in humidified air. Matured oocytes were fertilized with 1-2 × 10 6 /mL of frozen-thawed bull semen. At the end of the period the embryos were divided into two subgroups in order to be developed. Therefore, four groups were established: Group AI-SOF (synthetic oviduct fluid), Group AII-CR1aa (Charles Rosencrans), Group BI-SOF, and Group BII-CR1aa. The rates of blastocyst development and expanded blastocysts were detected as 20%, 18.1%, 24.6%, and 22.5% and survival rate as 5.5%, 23.6%, 0%, and 5.2%, respectively, showing statistically significant superiority in Group AII-CR1aa at the level of P < 0.05. In conclusion, supplementing cysteamine into maturation media with subsequent culture in AII-CR1aa had a positive effect on blastocyst survival after vitrification.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Vitrification of in vitro-produced bovine embryos matured inmodified TCM-199 medium

Sandal¹,

Özdaş²

2015

Turk J Vet Anim Sci

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“…In this context, the addition of IGF-I to the IVP media has been evaluated in many species, such as cattle (Neira et al 2010), buffalo (Singhal et al 2009) and caprine (Zhou & Zhang 2005). In these studies positive effects of IGF-I addition has been demonstrated and the concentration of the hormone used varied from 50 to 100ng/mL.…”

Section: Discussionmentioning

confidence: 99%

Porcine follicular fluid concentration of free insulin-like growth factor-I collected from different diameter ovarian follicles

et al. 2013

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The study aimed to quantify the concentrations of free IGF-I in serum and fluid of ovarian follicles in pre-pubertal gilts and describe the ovarian morphology by measuring the size of the ovaries and counting the number of surface follicles. Ovaries (n=1,000) from pre-pubertal gilts were obtained immediately after slaughter. A total of 10 samplings were performed, with ovaries obtained from 50 females for each collection. The follicles situated on the surface of each ovary were classified as small (SFs, 2 to 5mm in diameter) or large (LFs 6 to 10mm in diameter) and the follicular fluid was obtained by follicle aspiration. The collection of serum samples was performed after the gilts exsanguination using sterile tubes. From the pool of serum and follicular fluid obtained from 50 females, the concentration of free IGF-I was determined in each sample using an enzyme immunoassay kit (ELISA). The description of ovarian morphometry was performed in 100 ovaries from randomly selected gilts. The larger and smaller lengths of ovaries were measured, and the total number of SFs and LFs present on the surface of each ovary were also counted. The IGF-I concentration was greater (P<0.05) in LFs (170.92±88.29 ng/mL) compared with SFs (67.39±49.90ng/mL) and serum (73.48±34.63ng/mL). The largest and smallest length of the ovaries was 26.0±3.0 and 19.0mm ±2.0mm, respectively. The number of SFs (70.86±25.76) was greater (P<0.01) than LFs (6.54±5.26). The study concluded that LFs present greater levels of IGF-I when compared with SFs and blood, which is related to increased activity of the LFs and its differentiation to ovulation. In addition, ovaries of pre-pubertal gilts have a higher number of SFs compared to LFs. Therefore, our study demonstrated unique data regarding the physiological concentration of free IGF-I in ovarian follicles, that can be used in future research to evaluate the addition of this hormone in the in vitro production media of porcine embryos with the goal to improve the technique efficiency.

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“…The cumulus oocytes complexes were graded as: A Grade: having evenly granulated homogenous ooplasm with cumulus cells of three or more compact layers, B Grade: having homogenous ooplasm with two to three layers of cumulus cells, C Grade: having irregular ooplasm with less compact cumulus cells and D Grade: having irregular dark ooplasm and highly expanded cumulus cells (Singhal et al, 2009).…”

Section: Classification Of Oocytesmentioning

confidence: 99%

Effect of melatonin on maturation capacity and fertilization of Nili-Ravi buffalo (<i>Bubalus bubalis</i>) oocytes

Nagina¹,

Asima²,

Nemat³

et al. 2016

Open Vet J.

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This study evaluated the effect of melatonin supplementation of in vitro maturation media on in vitro maturation (IVM) and in vitro fertilization (IVF) rate of buffalo oocytes. Cumulus oocytes complexes (COCs) were aspirated from follicles of 2-8 mm diameter. In experiment I, COCs were matured in IVM medium supplemented with 0 (control), 250, 500, and 1000 μM melatonin for 22-24 hours in CO 2 incubator at 38.5°C with 5% CO 2 and at 95% relative humidity. The maturation rate did not differ in media supplemented with melatonin at 250 μM, 500 μM, 1000 μM and control (0 μM). In experiment II, the matured oocytes were fertilized in 50 μl droplets of Tyrode's Albumin Lactate Pyruvate (TALP) medium having 10 ug/ml heparin for sperm (2 million/ml) capacitation. The fertilization droplets were then kept for incubation at 5% CO 2, 39°C and at 95% relative humidity for 18 hours. The fertilization rate was assessed by sperm penetration and pronuclear formation. Fertilization rate was improved when maturation medium was supplemented with 250 μM melatonin compared to control. In conclusion, melatonin supplementation to serum free maturation media at 250 μM improved the fertilization rate of buffalo oocytes.

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Effect of including growth factors and antioxidants in maturation medium used for in vitro culture of buffalo oocytes recovered in vivo

Cited by 28 publications

References 29 publications

Vitrification of in vitro-produced bovine embryos matured inmodified TCM-199 medium

Vitrification of in vitro-produced bovine embryos matured inmodified TCM-199 medium

Porcine follicular fluid concentration of free insulin-like growth factor-I collected from different diameter ovarian follicles

Effect of melatonin on maturation capacity and fertilization of Nili-Ravi buffalo (<i>Bubalus bubalis</i>) oocytes

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