Effect of inclusion of porcine mucosa hydrolysate in diets varying in lysine content on growth performance and ileal histomorphology of broilers

Mateos, G. G.; Mohiti-Asli, M.; Borda, E.; Mirzaie, S.; Frikha, Mohamed

doi:10.1016/j.anifeedsci.2013.09.013

Cited by 22 publications

(15 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This helps to explain, along with the short experimental period (9 days), why the EDG of the piglets showed no difference among the dietary groups. However, the P50 group increased the diarrhea rate, perhaps because of the high salt content and poor carriers added to co-dried P50, which is the porcine mucosa hydrolysate after heparin extraction and is usually sprayed into a soybean meal carrier (16, 17, 36). Additionally, the reduced AID of CP in the P50 group in our previous study (21) could also explain the increased diarrhea rate, because more undigested protein flow into the hindgut for protein fermentation.…”

Section: Discussionmentioning

confidence: 99%

“…Although enzyme-treated soybean meal (14), cottonseed protein (15), dried porcine solubles (16, 17), and fish meal (18) are widely used in piglets' diets, few studies have evaluated the microbial mechanism of these protein sources on protein fermentation in the hindgut. Therefore, this study investigated the effects of P50, S50, CDCP, and FM on the growth performance, AID of CP, colonic mucin chemotypes, and the composition and metabolites of colonic microbiota in piglets.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Colonic Microbiota and Metabolites Response to Different Dietary Protein Sources in a Piglet Model

Chang

Hou

et al. 2019

Front. Nutr.

View full text Add to dashboard Cite

Dietary protein sources have the potential to affect the colon microbiome of piglets that will subsequently have a large impact on metabolic capabilities and hindgut health. This study explored the effects of different protein sources on the growth performance, diarrhea rate, apparent ileal digestibility (AID) of crude protein (CP), colonic mucin chemotypes, colonic microbiome, and microbial metabolites of piglets. Twenty-four piglets were randomly divided into four groups that received isoenergetic and isonitrogenous diets containing either Palbio 50 RD (P50), Soyppt-50% (S50), concentrated degossypolized cottonseed protein (CDCP), or fish meal (FM) as the sole protein source. The experimental diets did not affect the estimated daily gain (EDG), but P50 increased fecal score compared with S50 and CDCP. CDCP increased, but P50 reduced AID of CP in comparison to FM and S50. S50 and CDCP increased the amount of mixed neutral-acidic mucins relative to P50. Venn analysis identified unique OTUs in the P50 (13), CDCP (74), FM (39), and S50 (31) groups. The protein sources did not change the colonic bacterial richness or diversity. High Escherichia abundance in the P50 and FM, great abundant of Lactobacillus in the CDCP, and high Gemmiger abundance in the S50 were found. The CDCP tended to elevate valeric acid and branched chain fatty acid (BCFA) concentrations compared with the other diets. The P50 and FM groups had greater ammonia nitrogen and methylamine contents than the S50 and CDCP groups. There was a positive correlation between the Escherichia and ammonia nitrogen, the Lactobacillus and short chain fatty acid (SCFA), and a negative correlation between the Gemmige and BCFA. These findings suggested short-term feeding of different protein sources did not affect the piglets' growth, but P50 increased the diarrhea rate. Potential pathogenic bacteria and detrimental metabolites appeared in the colons of piglets fed P50 and FM, whereas, beneficial effects were conferred upon piglets fed CDCP and S50, thus indicating that available plant proteins (cotton seed, soy) added to the diets of piglets enhanced colon health by reducing protein fermentation.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Colonic Microbiota and Metabolites Response to Different Dietary Protein Sources in a Piglet Model

Chang

Hou

et al. 2019

Front. Nutr.

View full text Add to dashboard Cite

show abstract

“…P50 as a dried porcine soluble containing lots of peptides should own high protein digestibility in small intestine due to greater utilization of peptides than intact proteins and free amino acids (Myers et al, ). However, Mateos, Mohiti‐Asli, Borda, Mirzaie, and Frikha () reported that P50 as a popular DPS was produced by spraying the porcine mucosa hydrolysate into SBM carriers, which might contribute to low protein digestibility. Likewise, we identified fiber fractions in P50 ingredient, which demonstrates it contains plant‐based carriers.…”

Section: Discussionmentioning

confidence: 99%

Effects of different protein sources completely replacing fish meal in low‐protein diet on growth performance, intestinal digestive physiology, and nitrogen digestion and metabolism in nursery pigs

Hou

Song

et al. 2019

Animal Science Journal

View full text Add to dashboard Cite

The study compared the effects of selected proteins replacing fish meal in low‐protein diets on piglets’ growth performance, intestinal digestive physiology, and nitrogen digestion and metabolism. Five reduced CP, amino acid (AA)‐supplemented diets containing 4% of either S50, HP300, concentrated degossypolized cottonseed protein (CDCP), P50, or fish meal were assigned to six pens with 11 pigs for a 28‐day study period. Compared with fish meal, dietary proteins did not affect growth performance, apparent total tract digestibility (ATTD) of nutrients, serum hormone levels and biochemical parameters, apparent ileal digestibility (AID) of CP and most AA, colonic short‐chain fatty acid (SCFA) contents, duodenal and ileal morphology, digestive enzyme activity, and pH in small intestine of piglets. However, HP300, CDCP, and P50 decreased (p < 0.05) fecal N excretion per weight gain. AID of Ile in S50 and HP300 and Glu in P50 were improved (p < 0.05), and AID of Gly in other proteins was reduced (p < 0.05). S50 and P50 lowered (p < 0.05) the contents of colonic isobutyric and isovaleric. S50 and HP300 reduced (p < 0.05) jejunal villus height. CDCP increased (p < 0.05) the pepsin activity in stomach. S50, HP300, and CDCP decreased (p < 0.05) pH in the proximal colon. Overall, the selected proteins could completely replace fish meal in low‐protein diet without impairing piglets’ growth via maintaining intestinal digestive physiology, and nitrogen digestion and metabolism.

show abstract

“…Hydrolysed porcine mucosa (HPM) is a new type of functional nutritive animal protein source. It is derived from cleaned small‐intestinal mucosa of pigs and is a by‐product of the production of heparin sodium, an anticoagulant drug (Mateos et al., 2014). In the process of extracting heparin sodium, enzymes are added to obtain more products (Hai et al., 2013).…”

Section: Introductionmentioning

confidence: 99%

Addition of enzyme‐digested hydrolysed porcine mucosa to low‐fishmeal feed improves growth, intestinal microbiota, and intestinal peptide and amino acid transporter expressions in hybrid groupers ( Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂)

et al. 2020

View full text Add to dashboard Cite

In this study, five iso-nitrogenous and iso-lipidic experimental low-FM diets were formulated with enzyme-digested hydrolysed porcine mucosa (EH) at concentrations of 0 g/kg (EH0, controls), 30 g/kg (EH3), 60 g/kg (EH6), 90 g/kg (EH9) and 120 g/kg (EH12). Juvenile hybrid groupers (n = 450; initial body weight 7.50 ± 0.02 g) were randomly divided into 15 tanks and fed the experimental diets twice daily for 56 days. The weight gain rate, specific growth rate, protein efficiency rate, feed conversion rate and feed intake rate of group EH3 were not significantly different from those of controls. The villus length and muscle thickness of the distal intestine (DI) in group EH12 and the villus width of the proximal intestine (PI) in group EH6 were significantly higher than those in controls. The Shannon index was significantly higher in group EH6 than in other groups. Group EH6 exhibited 17 pathway subclasses with significant differences to the other groups, comprising organismal systems (4), human diseases (6), cellular processes (2), metabolism (4) and environmental information processing (1). The expressions of CAT1, CAT2, LAAT1 and SNAT3 in the PI, and PAT1, B 0 AT3 and B0, +AT in the mid intestine (MI), and EAAT1 and PepT1 in the PI and MI in group EH6 were significantly higher than those in controls. The expressions of CAT1, CAT2, LAAT1, PAT1, SNAT3, EAAT1, B 0, + AT and PepT1 in the DI of group EH3 were significantly greater than those in controls. The maximum amount of EH that could be added to low-FM feed to improve the digestive enzyme activity and amino acid and small peptide transport ability of the DI without obviously affecting the growth performance of hybrid groupers was 30 g/kg. EH contents ≥ 60 g/kg had negative impacts on growth performance and feed efficiency.

show abstract

Effect of inclusion of porcine mucosa hydrolysate in diets varying in lysine content on growth performance and ileal histomorphology of broilers

Cited by 22 publications

References 27 publications

Colonic Microbiota and Metabolites Response to Different Dietary Protein Sources in a Piglet Model

Colonic Microbiota and Metabolites Response to Different Dietary Protein Sources in a Piglet Model

Effects of different protein sources completely replacing fish meal in low‐protein diet on growth performance, intestinal digestive physiology, and nitrogen digestion and metabolism in nursery pigs

Addition of enzyme‐digested hydrolysed porcine mucosa to low‐fishmeal feed improves growth, intestinal microbiota, and intestinal peptide and amino acid transporter expressions in hybrid groupers ( Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂)

Contact Info

Product

Resources

About