Effect of Increasing Dietary Protein from Soybean Meal on Intestinal Microbiota and Their Fatty Acids Production in Broiler Chicken

Abstract

To evaluate the effect of increasing dietary protein from soybean meal on chicken growth, three treatments of the control (22% CP), additional crude protein diet (28% CP) and additional essential amino acid diet (22% CP with high dose of EAA) were fed for 900 broiler chickens with an age average of 1-21 days. Twenty birds per treatment were randomly sampling for the effects on intestinal villi, gut microbiota and short chain fatty acid production. Results indicated high protein diet had no effect on chicken growth, however, it decreased the villi height and Lactobacilli in jejunum of chicken. Interestingly, the enrichment of CP and EAA supplemented diet promoted especially Clostridium coccoides-Eubacterium rectal group for two times of the control in the ileum of chicken. While the suppression of Campylobacter in cecum and undetectable Clostridium perfringens were observed. There were no significant different for microbial short chain fatty acid production in intestine. The concentrations of lactic acid, propionic acid and acetic acid detected from the control and these two treatments were not significantly different. On the basis of these findings, high protein diet from soybean meal affected broiler gut microbiota especially the increasing of Clostridium coccoides-Eubacterium rectal group, but did not support their growth performance and intestinal metabolites.All copyrights reserved to Nexus® academic publishers

Caeca Microbial Variation in Broiler Chickens as a Result of Dietary Combinations Using Two Cereal Types, Supplementation of Crude Protein and Sodium Butyrate

“…Both families are major members of the phylum Firmicutes in the ceca; however, Ruminococcaceae is more abundant in birds with low feed conversion ratios ( Singh et al, 2012 ) that can be associated in the present study to the lower levels of CP. The responses obtained by these family members confirm microbiota’s significant impact on feed digestion and assimilation of dietary components ( Nakphaichit, 2014 ).…”

“…This study showed the influence of CP content on the microbial structure. Crude protein is essential in chicken metabolism since it constitutes the source of amino acids that are further absorbed and transformed into body proteins ( Nakphaichit, 2014 ). Family Ruminococcaceae, recognized as a late colonizer of the chicken caeca ( Richards et al, 2019 ), and family Lachnospiraceae were more abundant in the low level CP diets.…”

Advances in Enteropathogen Control Throughout the Meat Chicken Production Chain

“…In another study, a high-crude protein diet did not affect cecal Campylobacter in unchallenged broilers when fed from day 1 to 21, but a diet supplemented with essential amino acids (methionine, lysine and threonine) reduced count to below detection (control count was 5 log10 CFU/g) [324]. Increasing threonine content in feed by 12% had no significant effect on cecal S.Enteritidis in challenged birds [24] and no significant effect on cecal S.Typhimurium was seen in broilers given feed containing added glutamine at 4, 10 or 11 days post-challenge [325].…”

Advances in enteropathogen control throughout the meat chicken production chain

“…In another study, a high‐crude‐protein diet did not affect cecal Campylobacter in unchallenged broilers when fed from day 1 to 21, but a diet supplemented with essential amino acids (methionine, lysine, and threonine) reduced count to below detection (control count was 5 log 10 CFU/g) (Nakphaichit et al., 2014). In another study, increasing threonine content in feed by 12% had no significant effect on cecal S .…”