Effect of Incubation Time, CuSO4 and Glucose Concentrations on Biosynthesis of Copper Oxide (CuO) Nanoparticles with Rectangular Shape and Antibacterial Activity: Taguchi Method Approach

Abstract

In nanotechnology, using of metallic nanoparticles (MNPs) has obtained major attention for over a century because of their unique properties in nano scale. In this paper, the effect of three factors (incubation time, concentrations of copper sulfate and glucose) on the biosynthesis of copper (II) oxide nanoparticles (CuO NPs) by Halomonas elongata IBRC-M 10214 and the antibacterial activity of nanoparticles were evaluated. Experiment design and analysis of Taguchi method were carried out by Qualitek-4 software. Effect of CuSO4 concentration (1.4, 2.8 and 5.6 mM), incubation time (48, 72 and 96 h) as three different levels was measured as three major factors in the biosynthesis of metallic nanoparticles. Synthesized CuO nanoparticles were characterized by utilizing ultraviolet-visible (UVVis) spectroscopy, X-ray diffraction (XRD), Fourier transform infra-red (FT-IR) spectroscopy and field emission scanning electron microscope (FESEM) techniques. Among the three factors, results illustrated that considerable effect was related to CuSO 4 concentration. These analyses demonstrated that the average CuO nanoparticles crystalline size was 57-79 nm with rectangular shape. Also, for evaluating of antibacterial effects, maximum zone of inhibition, two important multidrug resistant pathogenesis bacteria, Escherichia coli ATCC 25922, and Staphylococcus aureus ATCC 43300 were used. Antibacterial assay of CuO nanoparticles showed antibacterial activity toward the pathogenic bacterial strains of E. coli by 5 mm and S. aureus by 5.5 mm for maximum zone of inhibition. In conclusion, this study presented simple, low expensive, eco-friendly and high productivity in the fabrication of CuO nanoparticles. In addition, these metallic nanoparticles had antibacterial effect that could be usable in medicinal aspect for fighting against prominent pathogen bacteria such as E. coli ATCC 25922 and S. aureus ATCC 43300.

Biosynthesis of Metal Nanoparticles via Microbial Enzymes: A Mechanistic Approach

“…Aeromonas hydrophila is a gram-negative bacterial strain that is used for the biosynthesis and antimicrobial applications of ZnO nanoparticles [37]. Recently, triangular CuO nanoparticles were produced using Halomonas elongate , and their antimicrobial activity was confirmed against E. coli and S. aureus [38]. In another recent report, super paramagnetic iron oxide nanoparticles (29 nm) were produced using Bacillus cereus and they were reported for their anti-cancer effects against the MCF-7 and 3T3 cell lines in a dose-dependent manner [39].…”

Optimal conditions for levan biopolymer production and its use in the synthesis of bactericidal levan-ZnO nanocomposite

“…Recent developments in the field of nanotechnology, especially in the making of nanoparticles of different shapes and sizes, have led to the establishment of a new group of antibacterial agents (Helmlinger et al, 2016;Taran et al, 2016). Nanoparticles have a higher surface per volume ratio compared to larger particles with the same chemical composition, which makes them more active biologically (Rad et al, 2018). Nanocomposites are nanostructures that have been regarded and studied as a new class of antibiotics in recent years due to their unique features.…”

Bacterial synthesis of nanoparticles: A green approach

“…Thus, ZnO nanoparticles were synthesized with the participation of Lactobacillus plantarum (Selvarajan & Mohanasrinivasan, 2013) and Aeromonas hydrophila (Jayaseelan et al, 2012). Antimicrobial activity against E. coli and S. aureus is exhibited by CuO nanoparticles formed with the participation of Halomonas elongata (Rad et al, 2018). Iron oxide nanoparticles obtained using Bacillus cereus had dose-dependent anticancer effects against MCF-7 and 3T3 cell lines (Fatemi et al, 2018).…”