Effect of Individual, Simultaneous and Sequential Inoculation of Pseudomonas fluorescens and Meloidogyne incognita on Growth, Biochemical, Enzymatic and Nonenzymatic Antioxidants of Tomato (Solanum lycopersicum L.)

Noureldeen, Ahmed; Asif, Mohd; Ansari, Taruba; Khan, Faryad; Shariq, Mohammad; Ahmad, Faheem; Mfarrej, Manar Fawzi Bani; Khan, Amir; Tariq, Moh; Siddiqui, Mansoor A.; Al-Barty, Amal; Darwish, Hadeer

doi:10.3390/plants10061145

Cited by 13 publications

(4 citation statements)

References 44 publications

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“…Zhou et al (2016) observed for the first time the nematicidal capacity of B. met about M. incognita, however, the strain used in the present assay did not show nematicidal activity in the control of M. enterolobii. Meloidogyne incognita and P. fluorescens in simultaneous inoculations did not efficiently reduce the number of egg mass in tomato root, however, the reduction was greater when the inoculation scheme was P. fluorescens and after 15 days inoculated: M. incognita (Noureldeen et al, 2021). The reproduction factor was higher in tomato plants inoculated with M. javanica followed by inoculation with Paecilomyces lilacinus with an interval of 10 days compared the simultaneous inoculations of the organisms and the inoculation of P. lilacinus 10 days before inoculation with M. javanica (Ganaie & Khan, 2010).…”

Section: Discussionmentioning

confidence: 84%

Biotic Stress Caused by Meloidogyne Enterolobii in Physiology of Guava Tree Treated With Bacillus Methylotrophicus

Souza¹,

Pontes²,

Furlanetto³

et al. 2023

JASR

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The interference of nematodes in the physiology of plants is little studied, as well as the ability of rhizobacteria to mitigate the physiological damage caused by nematodes. The objectives of this research were to monitor physiological changes in guava plants parasitized by Meloidogyne enterolobii and treated with bionematicide, active ingredient: Bacillus methylotrophicus (B.met) and to evaluate the efficiency of the bionematicide in controlling the nematode. The experiment was installed in a greenhouse and the treatments represented by: 1) 'Pedro Sato´ guava not inoculated and not treated with B. met (control); 2) 'Paluma' guava not inoculated and not treated with B. met (control); 3) `Pedro Sato´ not inoculated and treated with B. met; 4) `Paluma´ not inoculated and treated with B. met; 5) `Paluma´ inoculated and not treated with B. met; 6) `Pedro Sato´ inoculated and not treated with B. met; 7) `Pedro Sato´ inoculated and treated with B. met and 8) `Paluma´ inoculated and treated with B. met. Four applications of bionematicide were performed at a dosage of 3 mL/L of water per plant. Between the second and third application, the plants were inoculated with 5000 eggs and juveniles of M. enterolobii. Seven assessments of physiological parameters were performed. At 132 days after inoculation (DAI) the roots were weighed and the gall index, egg mass index and reproduction factor were determined. Meloidogyne enterolobii and B. met combined reduced the stomatal conductance and transpiration at 26 and 44 DAI, respectively. Photosynthesis was lower at 26 DAI in treatments that received the nematode and bacteria combined. Meloidogyne enterolobii and B. met isolated and combined reduced water use efficiency at 26 and 44 DAI. In root mass there was no difference between treatments. Gall index, egg mass index and reproduction factor were higher in treatments that received nematodes, regardless of the application with the bacteria.

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Section: Discussionmentioning

confidence: 84%

Biotic Stress Caused by Meloidogyne Enterolobii in Physiology of Guava Tree Treated With Bacillus Methylotrophicus

Souza¹,

Pontes²,

Furlanetto³

et al. 2023

JASR

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“…This is in agreement with the result obtained in the present study. The mechanism of antagonism of rhizosphere bacteria against plant-parasitic nematodes might be due to toxic metabolites or antibiotics 21,22 . Besides, proper management of fungal pathogens by fluorescent pseudomonads is due to the production of toxic metabolites, antibiotics, or siderophores 23,24 .…”

Section: Resultsmentioning

confidence: 99%

Biological Control of Meloidogyne incognita on Eggplant (Solanum melongena)

Aminisarte¹

2021

Asian J. of Plant Sciences

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Background and Objective: Pseudomonas fluorescens is an essential group of growth-promoting bacteria that can directly or indirectly improve plant growth. Several studies showed they could limit or suppress root-knot nematode. In this study, the effect of ten Iranian isolates of P. fluorescens (M1, M2, M3, M4, M5, M6, M7, M8, M9, M10) was assessed on eggplant growth parameters and the management of the root-knot nematode (Meloidogyne incognita) under greenhouse condition. Materials and Methods: The M. incognita was reared in tomato seedlings. The bacteria inoculum was reared in tryptic soy broth. Hydrogen cyanide and protease were assessed on the plate (9 cm diameter) include King B and Skim Milk Agar (SMA), respectively, in a dark situation. The greenhouse experiment was done to evaluate the effect of P. fluorescens isolates on M. incognita and plant parameters. The data was analyzed statistically using SAS software. Results: Eggplant seedlings were inoculated with ten P. fluorescens isolates in the presence or absence of M. incognita. The result displayed P. fluorescens isolate M 1 significantly improved the plant growth parameters and reduced the nematode reproductive factors for M. incognita associated with eggplant. In vitro results showed that isolates M 1 , M 3 , M 6 and M 7 caused significantly (p<0.05) more nematode mortality than the other isolates and the control. Conclusion: Based on the present study result, P. fluorescens isolate M 1 seems to be the best applicant for improving the growth and biological control of M. incognita in eggplant crops under greenhouse situations.

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“…The change in the content of MDA (a product of lipid peroxidation) serves as an indicator of the level of oxidative damage that a plant has suffered. The reduction in the MDA content following the treatment with the beneficial microorganisms or another sustainable method (the application of humic acid) has been found to correlate with an increase in antioxidant enzymes [ 95 , 114 , 115 ]. Several fungi or bacteria act as BCAs, whether directly by reducing nematode development and/or indirectly by stimulating the plant host defense mechanisms involving mainly antioxidant defense enzymes [ 114 , 116 , 117 ].…”

Section: Discussionmentioning

confidence: 99%

Comparative Effectiveness of Filamentous Fungi in Biocontrol of Meloidogyne javanica and Activated Defense Mechanisms on Tomato

Hajji-Hedfi¹,

Hlaoua

Al-Judaibi

et al. 2022

JoF

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The nematicidal potential of five filamentous fungi as biological control agents (BCAs) against the root-knot nematode (RKN), Meloidogyne javanica, infecting tomato was assessed in vitro and in pot experiments. The five promising native taxa, namely Trichoderma longibrachiatum, T. harzainum, T. asperellum, Lecanicillium spp., and Metacordyceps chlamydosporia, were selected to compare their effectiveness against both chemical (Mocap, 10% ethoprophos) and biological (abamectin) nematicides on M. javanica reproduction indices and plant growth parameters. The stimulation of defense mechanisms was assessed by monitoring changes in the enzymatic activities of the polyphenol oxidase (PPO), peroxidase (POD), ascorbate peroxidase (APX), catalase (CAT), lipid peroxidation (MDA), phenols, and proteins content of tomato roots. The laboratory assays revealed that T. longibrachiatum, M. chlamydoporia, and Lecanicillium spp. seemed to be the most effective under laboratory conditions, with more than 60% of juvenile mortality. The egg infection rate was above 62%, and the egg hatching rate was below 32%. The direct parasitism by the five taxa was confirmed by scanning electron microscope observation. The results of this study found a similar parasitism mechanism for T. longibrachiatum, T. harzianum, and M. chlamydosporia, where their hyphae and spores adhered to the M. javanica juveniles cuticle layer and formed trapping rings around them. The pot experiment results showed that T. harzianum and Lecanicillium spp. enhanced the plant growth parameters. Trichoderma longibrachiatum, abamectin, and the ethoprophos-based nematicides effectively decreased the reproduction rates of the nematode. The Trichoderma species and M. chlamydosporia significantly reduced the gall index and female fecundity of RKN. The treatment with BCAs and chemical nematicides involved a significant increase in the antioxidant activities of nematode-infected plants. The ethoprophos and fungal treatments decreased the MDA and total phenols content compared with the nematode-infested seedlings. This paper analyzes the advancements made towards the effective and efficient biocontrol of M. javanica using different fungal taxa, especially T. longibrachiatum and M. chlamydosporia, and the implications of these advancements for sustainable agriculture and food security.

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Effect of Individual, Simultaneous and Sequential Inoculation of Pseudomonas fluorescens and Meloidogyne incognita on Growth, Biochemical, Enzymatic and Nonenzymatic Antioxidants of Tomato (Solanum lycopersicum L.)

Cited by 13 publications

References 44 publications

Biotic Stress Caused by Meloidogyne Enterolobii in Physiology of Guava Tree Treated With Bacillus Methylotrophicus

Biotic Stress Caused by Meloidogyne Enterolobii in Physiology of Guava Tree Treated With Bacillus Methylotrophicus

Biological Control of Meloidogyne incognita on Eggplant (Solanum melongena)

Comparative Effectiveness of Filamentous Fungi in Biocontrol of Meloidogyne javanica and Activated Defense Mechanisms on Tomato

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