The present investigation entitled "Effect of organic media, plant growth regulators and different colour of wrappers on success and survival of air layering of Guava (Psidium guajava L.)” Was carried out in Month of August during 2019 and 2020 at the Fruit Research Station Entkhedi, Bhopal, (M.P.) under the R.A.K. College of Agriculture, Sehore. The experiment was laid down in Randomized Block Design with three replication and twenty-five treatments. In these treatments five colour of Poly wrappers was used i.e., White, Black, Blue, Red and Green, five concentrations of Growth regulators i.e., IBA 0 ppm, IBA 2500 ppm, IBA 5000 ppm, IBA 7500 ppm and IBA 10000 ppm and four types of media i.e., Vermicompost, Soil, Leaf manure and Sphagnum moss. Observations was recorded on rooting studies like callus formation day taken from layering date, rooting percentage success, average number of primary roots per air-layer, average number of secondary roots per air-layer, average length of primary roots, average length of secondary roots, average diameter of primary roots, average fresh weight of roots and average dry weight of roots. Result obtained that the treatment T21 (IBA 10000 PPM + Vermicompost + W5) found superior in all the parameters of rooting studies during 2019-20 and 2020-21, and also in pooled data.