Effect of induced changes in membrane permeability on the defence response of Chlorella vulgaris to infection by Acholeplasma laidlawii

Loseva, N.L.; Gordon, L. Kh.; Alyabyev, A.Ju.; Andreyeva, I.N.; Kolesnikov, O. P.; Чернов, В. М.; Пономарева, А. А.; Кемп, Р. Б.

doi:10.1016/j.tca.2004.06.023

Cited by 11 publications

(5 citation statements)

References 36 publications

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“…3B), which can be interpreted as a stimulation of the mitochondrial activity and the data could support the hypothesis that another important source of energy for the microalgal cells might proceed from respiration (Loseva et al, 2004). As mentioned before, ETFA and ETFB genes, involved in the respiratory electron transport of the mitochondria, were upregulated.…”

Section: Transcriptomic Analysessupporting

confidence: 82%

“…The mechanism of action of atrazine affects the photosynthesis, but could also provoke perturbations on mitochondrial respiratory chain. Several authors reported that physiologically, mitochondrial hyperpolarization could be due to an increase in respiration, with a higher electron flux, more active oxidative phosphorylation and consequently, possible leaks of electrons that favored the generation of ROS, as a by-product of the ATP-generating process (Huc et al, 2012;Loseva et al, 2004;Wallace, 2005). Atrazine toxicity on mitochondrial respiratory chain has been studied in murine and human cells (Chen et al, 2015;Koo et al, 2012).…”

Section: Cytoplasmic and Mitochondrial Membrane Potentialsmentioning

confidence: 99%

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Chlamydomonas reinhardtii cells adjust the metabolism to maintain viability in response to atrazine stress

et al. 2015

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Chlamydomonas reinhardtii cells were exposed to a sublethal concentration of the widespread herbicide atrazine for 3 and 24h. Physiological parameters related to cellular energy status, such as cellular activity and mitochondrial and cytoplasmic membrane potentials, monitored by flow cytometry, were altered in microalgal cells exposed to 0.25μM of atrazine. Transcriptomic analyses, carried out by RNA-Seq technique, displayed 12 differentially expressed genes between control cultures and atrazine-exposed cultures at both tested times. Many cellular processes were affected, but the most significant changes were observed in genes implicated in amino acid catabolism and respiratory cellular process. Obtained results suggest that photosynthesis inhibition by atrazine leads cells to get energy through a heterotrophic metabolism to maintain their viability.

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Section: Transcriptomic Analysessupporting

confidence: 82%

Section: Cytoplasmic and Mitochondrial Membrane Potentialsmentioning

confidence: 99%

Chlamydomonas reinhardtii cells adjust the metabolism to maintain viability in response to atrazine stress

et al. 2015

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“…A clear relationship between the levels of various stresses and plant energy metabolism has been demonstrated in recent times (Jia et al., 2003; Yang et al., 2003; Losevaa et al., 2004). Wang et al.…”

Section: Discussionmentioning

confidence: 92%

“…A clear relationship between the levels of various stresses and plant energy metabolism has been demonstrated in recent times (Jia et al, 2003;Yang et al, 2003;Losevaa et al, 2004). Wang et al (1995) reported that stress stimulation effectively increased mitochondrial activity, oxidative phosphorylation rates and ATP synthesis.…”

Section: Discussionmentioning

confidence: 95%

ATP‐induced Changes in Energy Status and Membrane Integrity of Harvested Litchi Fruit and its Relation to Pathogen Resistance

Jiang

et al. 2008

Journal of Phytopathology

140

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Litchi is a subtropical fruit of high commercial value on the international market but the fruit deteriorates rapidly after harvest due to rot development caused by Peronophythora litchii. To investigate the role of energy metabolism during disease development on harvested litchi fruit, fruits were dipped into solutions of either 0 or 1.0 mm adenosine triphosphate (ATP) for 3 min before being inoculated with Peronophythora litchii or not. Fruit were then stored for 6 days at 25°C and 90-100% relative humidity. Significant reductions in pericarp browning and disease severity and significant delays in membrane permeability and malondialdehyde (MDA) content were found in ATPtreated and P. litchii-inoculated fruit. Higher ATP concentrations and adenylate energy charge (EC) were observed in ATP-treated fruit. In addition, lower activities of phospholipase D, acid phosphatase and lipoxygenase enzymes involved in membrane lipid peroxidation and hydrolysis were recorded in ATPtreated fruit. Thus, treatment with ATP maintained higher energy levels, inhibited activities of the membrane hydrolysis-related enzymes, reduced membrane lipid peroxidation and helped maintain membrane integrity of the harvested litchi fruit at the early stage of storage, which could account for the inhibition of disease development of P. litchii-inoculated fruit.

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“…As a general feature, photosensitizing organisms react to the action of various pathogens as well as to other extreme factors with a cascade of stress responses. One of the nonspecific but important responses of cells under pathogen stress is the change in cellular membrane permeability resulting in the disruption of ion homeostasis (39,40). The presence of PEI, a cell permeabilizer, should affect this and allow deeper penetration of the photosensitizer into the cell.…”

Section: Discussionmentioning

confidence: 99%

Effect of Polyethylenimine, a Cell Permeabilizer, on the Photosensitized Destruction of Algae by Methylene Blue and Nuclear Fast Red

McCullagh

Robertson

2006

Photochem Photobiol

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The present study reports the effect a cell permeabilizer, polyethylenimine (PEI) has on the photodynamic effect of methylene blue (MB) and nuclear fast red (NFR) in the presence of hydrogen peroxide (H2O2). The photosensitized destruction of the algae Chlorella vulgaris under irradiation with visible light is examined. The photodynamic effect was investigated under aerobic and anaerobic conditions. The presence of a permeabilizer during the photosensitized destruction of C. vulgaris does not enhance the activity of the MB, MB/H2O2 system or the NFR, NFR/H2O2 system under aerobic conditions. However under anaerobic conditions we have determined that when a cell permeabilizer was added to the MB/H202 system, the photosensitized destruction of C. vulgaris proceeded via a combination of Type I and Type II mechanisms. The presence of PEI enforces MB/H2O2 to be active toward the destruction of C. vulgaris whether oxygen is present or absent. Under aerobic and anaerobic conditions the activity of NFR was suppressed in the presence of PEI as a result of electrostatic interactions between the photosensitizer and the cell permeabilizer. The decrease in fluorescence recorded is indicative of destruction of the chlorophyll a pigment.

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Effect of induced changes in membrane permeability on the defence response of Chlorella vulgaris to infection by Acholeplasma laidlawii

Cited by 11 publications

References 36 publications

Chlamydomonas reinhardtii cells adjust the metabolism to maintain viability in response to atrazine stress

Chlamydomonas reinhardtii cells adjust the metabolism to maintain viability in response to atrazine stress

ATP‐induced Changes in Energy Status and Membrane Integrity of Harvested Litchi Fruit and its Relation to Pathogen Resistance

Effect of Polyethylenimine, a Cell Permeabilizer, on the Photosensitized Destruction of Algae by Methylene Blue and Nuclear Fast Red

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