Effect of Inducers and PCBs on the Cytochrome P450 Enzymes in Cultured Quail Hepatocytes

Roelandt, L.; Dubois, Maryline; Todaro, Anna; Thomé, Jean-Pierre; Kremers, Pierre

doi:10.1006/eesa.1995.1057

Cited by 7 publications

(6 citation statements)

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“…Hepatic microsomes from female Japanese quail in our study formed 2␣-, 6␤-, 7␣-, 15␣-, 16␣-, 16␤-OHT, and androstenedione from testosterone. These results are in agreement with the testosterone metabolism by fetal Japanese quail hepatocytes in vitro [18]. The major difference between hepatic microsomes from adult female Japanese quail and fetal hepatocytes is the relatively lower formation rate of 2␣-OHT by the hepatic microsomes.…”

Section: Discussionsupporting

confidence: 88%

“…We have chosen to use the Japanese quail as a representative for a bird species because some information on hepatic P450 induction for this species is available. For instance, the induction of hepatic P450 activities after treatment of Japanese quail with other organochlorine contaminants [15,16], ␤-naphthoflavone and phenobarbital [17,18], has been reported.…”

Section: Introductionmentioning

confidence: 99%

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Induction of Hepatic Cytochrome P450 Activities by Toxaphene in Rat and Japanese Quail

Drenth

Oevelen

Buitenhuis

et al. 2000

Environ Toxicol Chem

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Abstract-Several organochlorine compounds can induce hepatic cytochrome P450 activities. We exposed female Japanese quail and female rats to a single oral dose of technical toxaphene ranging from 0.012 to 40 mg/kg body weight to investigate a possible dose-response relationship for P450 activity induction. The hepatic microsomal alkoxyresorufin dealkylation and steroid hydroxylation activities (testosterone, 17␤-estradiol) were determined. Only in the highest dose groups (40 mg/kg) of rats and Japanese quail were some P450 activities induced. Pentoxyresorufin-O-dealkylation, formation of 15␤-hydroxytestosterone, and formation of 2-hydroxyestradiol were induced significantly (p Յ 0.05) in rat hepatic microsomes with a factor of 3.9, 3.1, and 2.4, respectively. In Japanese quail hepatic microsomes, the formation rates of 6␤-, 15␣-, 16␤-hydroxytestosterone and androstenedione were induced significantly (p Յ 0.05) with a factor of 2.4, 6.5, 3.3, and 1.6, respectively. It is concluded that exposure to toxaphene can lead to specific P450 activity induction in rats and Japanese quail but only at doses near reported LD50 values. Because reported toxaphene levels in biota are lower compared with liver residue levels that we have measured in our study, we conclude that it is unlikely that P450 activity induction occurs in wildlife higher in the food chain due to environmental toxaphene exposure.

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Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Induction of Hepatic Cytochrome P450 Activities by Toxaphene in Rat and Japanese Quail

Drenth

Oevelen

Buitenhuis

et al. 2000

Environ Toxicol Chem

View full text Add to dashboard Cite

show abstract

“…Hepatic microsomes from female Japanese quail in our study formed 2α‐, 6β‐, 7α‐, 15α‐, 16α‐, 16β‐OHT, and androstenedione from testosterone. These results are in agreement with the testosterone metabolism by fetal Japanese quail hepatocytes in vitro [18]. The major difference between hepatic microsomes from adult female Japanese quail and fetal hepatocytes is the relatively lower formation rate of 2α‐OHT by the hepatic microsomes.…”

Section: Discussionsupporting

confidence: 86%

“…These results can be compared with a study using fetal Japanese quail hepatocytes in vitro exposed to phenobarbital. The formation rates of 6β‐, 16α‐, and 16β‐OHT were induced, while the formation rates of 2α‐, 7α‐, 15α‐OHT, and androstenedione remained unchanged [18]. So the P450 induction pattern of phenobarbital in fetal hepatocytes in vitro differs markedly from the in vivo induction pattern of toxaphene in adult female Japanese quail.…”

Section: Discussionmentioning

confidence: 99%

“…We have chosen to use the Japanese quail as a representative for a bird species because some information on hepatic P450 induction for this species is available. For instance, the induction of hepatic P450 activities after treatment of Japanese quail with other organochlorine contaminants [15,16], β‐naphthoflavone and pheno‐barbital [17,18], has been reported.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Induction of hepatic cytochrome p450 activities by toxaphene in rat and japanese quail

Drenth

Oevelen

Buitenhuis

et al. 2000

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

Several organochlorine compounds can induce hepatic cytochrome P450 activities. We exposed female Japanese quail and female rats to a single oral dose of technical toxaphene ranging from 0.012 to 40 mg/kg body weight to investigate a possible dose‐response relationship for P450 activity induction. The hepatic microsomal alkoxyresorufin dealkylation and steroid hydrox‐ylation activities (testosterone, 17β‐estradiol) were determined. Only in the highest dose groups (40 mg/kg) of rats and Japanese quail were some P450 activities induced. Pentoxyresorufin‐O‐dealkylation, formation of 15β‐hydroxytestosterone, and formation of 2‐hydroxyestradiol were induced significantly (p ≤ 0.05) in rat hepatic microsomes with a factor of 3.9, 3.1, and 2.4, respectively. In Japanese quail hepatic microsomes, the formation rates of 6β‐, 15α‐, 16β‐hydroxytestosterone and androstenedione were induced significantly (p ≤ 0.05) with a factor of 2.4, 6.5, 3.3, and 1.6, respectively. It is concluded that exposure to toxaphene can lead to specific P450 activity induction in rats and Japanese quail but only at doses near reported LD50 values. Because reported toxaphene levels in biota are lower compared with liver residue levels that we have measured in our study, we conclude that it is unlikely that P450 activity induction occurs in wildlife higher in the food chain due to environmental toxaphene exposure.

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The use of isolated hepatocytes to study the mechanisms of action of environmental contaminants

Reader

Denizeau

2000

The Hepatocyte Review

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Effect of Inducers and PCBs on the Cytochrome P450 Enzymes in Cultured Quail Hepatocytes

Cited by 7 publications

References 0 publications

Induction of Hepatic Cytochrome P450 Activities by Toxaphene in Rat and Japanese Quail

Induction of Hepatic Cytochrome P450 Activities by Toxaphene in Rat and Japanese Quail

Induction of hepatic cytochrome p450 activities by toxaphene in rat and japanese quail

The use of isolated hepatocytes to study the mechanisms of action of environmental contaminants

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