Effect of Induction of Late Embryonic Mortality on Plasma Profiles of Pregnancy Associated Glycoprotein 1 in Heifers

Szenci, O.; Beckers, Jean‐François; Sulon, Joseph; Bevers, M.M.; Börzsönyi, L; Fodor, László; Kovács, F.; Taverne, M.A.M.

doi:10.1016/s1090-0233(02)00180-6

Cited by 53 publications

(42 citation statements)

References 23 publications

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“…After diagnosing spontaneous cases of LEM by ultrasonography, both plasma bPSPB and bPAG1 levels began to decline in most cases, while the corpus luteum continued to produce progesterone [28,36,37]. This confirms the previous observations [35,52] and demonstrates that lower progesterone concentrations are not the cause of conceptus death.…”

Section: Diagnosis Of Pregnancy Lossessupporting

confidence: 84%

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Recent Possibilities for the Diagnosis and Pharmacological Control of Pregnancy Loss in Dairy Cow

Szenci¹

2015

JLS

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Abstract:One of the most recent techniques for the diagnosis of EP (early pregnancy) in cattle on the farm is B-mode ultrasonography. Under field conditions, acceptable results may be achieved with ultrasonography from days 25 to 30 post-AI. The reliability of the test greatly depends on the frequency of the transducer used, the skill of the examiner, the criterion used for a positive PD (pregnancy diagnosis) and the position of the uterus in the pelvic inlet. Pregnancy protein assays (PAG, PSPB) may provide an alternative method to ultrasonography for determining EP or LEM/EFM (late embryonic/early foetal mortality) in the cow. Although early pregnancy factor is the earliest specific indicator for fertilization, its detection is entirely dependend on the use of the RIT (rosette inhibition test), therefore its use in the field needs further inventions. Preventive pharmaceutical treatments with hCG (human chorionic gonadotropin), GnRH (gonadotropin realising hormone), PRID (progesterone-releasing intravaginal device) or CIDR (controlled internal drug release) inserts at different time periods at or post-AI may have some benefit in decreasing PL (pregnancy loss), however further examinations are warranted to determine how and when these treatments may influence PL in the field.

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Section: Diagnosis Of Pregnancy Lossessupporting

confidence: 84%

“…A further limitation after LEM is that protein levels may remain above the threshold level, although the concentration of both proteins decreases steadily [35,36]. This is probably related to the relatively long half-life (7-8 days for bPSPB and 3-4 days for bPAG1) in the maternal circulation after EM [35,37].…”

Section: Conceptus Proteins (Pspb Pag)mentioning

confidence: 99%

Recent Possibilities for the Diagnosis and Pharmacological Control of Pregnancy Loss in Dairy Cow

Szenci¹

2015

JLS

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“…In early pregnancy (after induced late embryonic death at days 30-38), the PAG-1 half-life is 3-4 days (Szenci et al 2003). A postpartal half-life of 8.4 days was observed by Sasser & Ruder (1987) and Kiracofe et al (1993).…”

Section: Discussionmentioning

confidence: 84%

The glycosylation of pregnancy-associated glycoproteins and prolactin-related protein-I in bovine binucleate trophoblast giant cells changes before parturition

Klisch¹,

Boos²,

Friedrich³

et al. 2006

Reproduction

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Binucleate trophoblast giant cells (BNC) in the bovine placenta produce glycoproteins, which are delivered into the mother after fusion of BNC with uterine epithelial cells. During most time of pregnancy, BNC produce pregnancy-associated glycoproteins (PAGs) and prolactin-related protein-I (PRP-I) with asparagine-linked lactosamine-type glycans terminating with N-acetyl-galactosamine. We show by lectin histochemistry that terminal N-acetyl-galactosamine (detected by Dolichos biflorus agglutinin, DBA) in placentomal BNC is greatly reduced prior to parturition, while lactosamine-type N-glycans (detected by Phaseolus vulgaris leucoagglutinin, PHA-L) remain unaltered. The change in DBA-staining showed no statistically significant differences between placentomes of cows with and without retention of fetal membranes. Western blots revealed that, at parturition the apparent molecular mass of PAGs and PRP-I is 1-2 kDa lower than in late pregnancy. These changes are due to alterations of asparagine-linked glycans, since the molecular weight of the peptide backbones after enzymatical release of asparagine-linked glycans is identical at late pregnancy and parturition. Lectin western blots showed a reduction of terminal N-acetyl-galactosamine on PAGs at parturition. A lectin sandwich-ELISA was used to differentiate DBA-and PHA-L-binding PAGs in sera of pregnant and non-pregnant cows. The values for DBA-binding PAGs at parturition were not significantly different from non-pregnancy, while the values for PHA-L-binding PAGs were significantly higher at parturition. The peripartal changes of PAG-and PRP-I-glycosylation could alter functional properties of these proteins and might therefore be considered for functional studies. The differentiation of PAG glycoforms in maternal serum could be valuable for a further optimization of PAG-based pregnancy diagnosis in cattle. Reproduction (2006) 132 791-798

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“…wood bison [107], moose [108], rocky mountain elk [109], white-tailed deer [110,111], mule deer [111], red deer [112], sika deer [113], fallow deer [114,115], mountain goat [116] and Alpine goats [117][118][119]. The measurement of PAG protein concentrations in maternal blood or milk of ruminants allow for 76.6-100% accuracy rates for early pregnancy diagnoses [120,121] and provide an alternative method to ultrasonography for determining late embryonic mortality [96,122].…”

Section: Pregnancy Diagnostic Pag Testsmentioning

confidence: 99%

Biodiversity of multiple Pregnancy-Associated Glycoprotein (PAG) family: gene cloning and chorionic protein purification in domestic and wild eutherians (Placentalia) – a review

2006

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-This review presents a broad overview of chorionic glycoproteins encoded by the Pregnancy-Associated Glycoprotein (PAG) gene family and also serves to illustrate how the recent discovery of the PAG family has contributed to our general knowledge of genome evolution, placental transcription and placental protein expression. The complex and large PAG family is restricted to the Artiodactyla order, although single PAG-like genes have also been identified in species outside the Artiodactyla. The PAGs are members of the aspartic proteinase (AP) superfamily. Unexpectedly, however, some members of the PAG family possess amino acid substitutions within and around the active site that likely render them unable to act as proteinases. This paper summarises the available information regarding biodiversity of PAG gene expression based on cDNA cloning, mRNA localisation studies and the structural organisation of the PAG genes with a particular emphasis on PAG promoters. It also compares available data regarding PAG protein purifications, sequencing and their N-glycodiversity. Finally, it discusses the scientific relevance, possible functional roles of the PAGs and describes possible profitable applications related to the detection of PAG proteins in the blood of pregnant domestic and wild species. chorionic glycoproteins / eutherian PAG family / diagnostic PAG markers

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Effect of Induction of Late Embryonic Mortality on Plasma Profiles of Pregnancy Associated Glycoprotein 1 in Heifers

Cited by 53 publications

References 23 publications

Recent Possibilities for the Diagnosis and Pharmacological Control of Pregnancy Loss in Dairy Cow

Recent Possibilities for the Diagnosis and Pharmacological Control of Pregnancy Loss in Dairy Cow

The glycosylation of pregnancy-associated glycoproteins and prolactin-related protein-I in bovine binucleate trophoblast giant cells changes before parturition

Biodiversity of multiple Pregnancy-Associated Glycoprotein (PAG) family: gene cloning and chorionic protein purification in domestic and wild eutherians (Placentalia) – a review

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