Effect of Inflammatory Mediators on the Electrophysiology of the Human Oviduct1

Abstract

The effects of histamine and other inflammatory mediators on the electrophysiology and intracellular free calcium ([Ca(2+)](i)) of human oviductal epithelial cells, grown as a polarized layer in primary culture, were studied. Transepithelial potential difference (PD) and short-circuit current (I(scc)) were recorded using a modified Ussing chamber. Resistance (R) was calculated from the measurements of PD and I(scc). Basally applied histamine produced transient increases in PD and I(scc) with a small decrease in R. The histamine effect was reduced by triprolidine (H(1) receptor antagonist) but was unaffected by H(2) (ranitidine) or H(3) (thioperamide) receptor antagonists. Blockers of Na(+), K(+), or Na(+)/K(+)/2Cl(-) channels did not affect histamine action. Blockers of Cl(-)/HCO(3)(-) channels or Ca(2+) channels reduced the histamine effect. Platelet activating factor (PAF), applied apically, increased PD and I(scc). Histamine produced a transient increase in fluorescence of Fura 2-AM dye, indicating an increase in [Ca(2+)](i). Triprolidine pretreatment inhibited histamine-stimulated [Ca(2+)](i) increase. Cimetidine, (H(2) receptor antagonist), ranitidine, or thioperamide reduced the histamine effect. Histamine increased contractions of both circular and longitudinal smooth muscles in oviduct segments, an effect that was antagonized by triprolidine or thioperamide but not by ranitidine. Histamine's action on Ca(2+) and Cl(-) movements may adversely affect oviductal fluid production and decrease fertility. PAF's effects on Cl(-) movements may be important for normal embryo transport.

Effect of histamine on the electrophysiology of the human parietal pleura

“…Conversely, histamine interferes with cellular transportation by regulating the ion transporters rather than altering the paracellular permeability and it increases electrical resistance of the human bronchial epithelial cells without increasing the permeability through the tight junctions, (Devalia et al 1994) an event which was also demonstrated in the porcine proximal colon cells (Ahrens et al 2003). Histamine also changes the electrochemical profile of fallopian tube cells and ovaries (Downing et al 1999(Downing et al , 2002 in a dose-dependent manner, while…”

“…H 1 receptors seem to play the most important role; in airway epithelia (Flynn et al 2009, Chan et al 1987, in vascular epithelia (Flynn et al 2009), in nostrils (Yang et al 2003), in Fallopian tubes and ovaries (Downing et al 1999(Downing et al , 2002 and in human colon (Keely et al 1995).…”

“…These effects are considered to be induced by histamines' interaction with its receptors, namely the H 1 and/or H 2 histamine receptors (Flynn et al 2009, Downing et al 1999.…”

Demilune Cell and Parotid Protein from Murine Oviductal Epithelium Stimulates Preimplantation Embryo Development

“…Its receptor is present in the oviducts of humans (43) and cows (44). Platelet-activating factor has been shown to affect the electrophysiology of human oviducts (45) and increase the intracellular calcium concentration (46) and proliferation (47) of bovine oviductal cells. In mares, embryonic prostaglandin E 2 has been proposed as the agent responsible for the differential transport of embryos and oocytes in the oviduct (48).…”

Histamine Stimulates Ciliary Beat Frequency via the H2 Receptor in the ProtochordateBotryllus schlosseri

“…They were then placed in a final volume of 30 μL of FSW in the middle of an area 1 cm 2 in diameter of a slide previously waterproofed with a ring of white vaseline. In the first series of experiments, the branchial portions were incubated for 10 min with various solutions (1, 10, 100, 1,000 μM) of histamine (Fluka) to identify the concentration of histamine at which the maximum response of the CBF occurs, as reported in vitro for mammalian ciliary epithelia (Lurie et al, ; Dowing et al, ; Del Donno et al, ). Then, the branchial portions were incubated with 30 μL of various solutions in FSW as follows: (1) FSW as control for 10 min; (2) a 100 μM solution of histamine for 10 min; (3) a 100 μM solution of a histamine receptor antagonist: chlorpheniramine maleate (CAS: 132‐22‐9) as HRH1 antagonist, ranitidine hydrochloride (CAS: 66357‐35‐5) as HRH2 antagonist, and clobenpropit dihydrobromide (CAS: 145231‐45‐4) as HRH3 antagonist.…”