Effect of Infused Ketone Bodies on Glucose Utilization in the Dog*

Felts, Philip W.; Crofford, O. B.; Park, C. R.

doi:10.1172/jci104949

Cited by 40 publications

(21 citation statements)

References 19 publications

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“…This finding is consistent with other reports of decreased plasma glucose concentration associated with elevated BHBA in pregnant sheep Harmeyer 2003, 2004), dogs (Felts et al, 1964;Madison et al, 1964), and pigs (Müller et al, 1984). However, our findings are in contrast to other reports that described a hyperglycemia in rabbits (Mirsky and Broh-Kahn, 1937) induced by ketone bodies infusion.…”

Section: Discussionsupporting

confidence: 93%

Long-term elevation of β-hydroxybutyrate in dairy cows through infusion: Effects on feed intake, milk production, and metabolism

Zarrin

Matteis

Vernay

et al. 2013

Journal of Dairy Science

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Elevation of ketone bodies in dairy cows frequently occurs in early lactation, usually concomitantly with a lack of energy and glucose. The objective of this study was to induce an elevated plasma β-hydroxybutyrate (BHBA) concentration over 48 h in mid-lactating dairy cows (i.e., during a period of positive energy balance and normal glucose plasma concentrations). Effects of BHBA infusion on feed intake, metabolism, and performance were investigated. Thirteen cows were randomly assigned to 1 of 2 infusion groups, including an intravenous infusion with Na-dl-β-OH-butyrate (1.7 mol/L) to achieve a plasma concentration of 1.5 to 2.0 mmol/L of BHBA (HyperB; n = 5), or an infusion of 0.9% saline solution (control; n = 8). Blood was sampled before and hourly during the 48 h of infusion. In the liver, mRNA transcripts related to gluconeogenesis (pyruvate carboxylase, glucose 6-phosphatase, mitochondrial phosphoenolpyruvate carboxykinase), phosphofructokinase, pyruvate dehydrogenase complex, and fatty acid synthesis (acetyl-coenzyme A carboxylase, fatty acid synthase) were measured by real-time PCR. Glyceraldehyde-3-phosphate dehydrogenase and ubiquitin were used as housekeeping genes. Changes (difference between before and after 48-h infusion) during the infusion period were evaluated by ANOVA with treatment as fixed effect, and area under the curve of variables was calculated on the second day of experiment. The plasma BHBA concentration in HyperB cows was 1.74 ± 0.02 mmol/L (mean ± SE) compared with 0.59 ± 0.02 mmol/L for control cows. The change in feed intake, milk yield, and energy corrected milk did not differ between the 2 experimental groups. Infusion of BHBA reduced the plasma glucose concentration (3.47 ± 0.11 mmol/L) in HyperB compared with control cows (4.11 ± 0.08 mmol/L). Plasma glucagon concentration in HyperB was lower than the control group. All other variables measured in plasma were not affected by treatment. In the liver, changes in mRNA abundance for the selected genes were similar between 2 groups. Results demonstrate that intravenous infusion of BHBA decreased plasma glucose concentration in dairy cows, but this decrease could not be explained by alterations in insulin concentrations or key enzymes related to gluconeogenesis. Declined glucose concentration is likely functionally related to decreased plasma glucagon concentration.

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Section: Discussionsupporting

confidence: 93%

Long-term elevation of β-hydroxybutyrate in dairy cows through infusion: Effects on feed intake, milk production, and metabolism

Zarrin

Matteis

Vernay

et al. 2013

Journal of Dairy Science

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show abstract

“…However, there was no decrease in urinary excretion of niacin and NMN in those fed high fat-high protein, niacin deficient diet (Diet 3). This may be due to the fact that , high amounts of tryptophan are available deleterious effects of high fat feeding on niacin biosynthesis are related to the high production of ketone bodies, which have been shown to be responsible for many deleterious effects on carbohydrate and fat metabolism reported earlier from this laboratory and confirmed by others (14,15).…”

Section: Resultssupporting

confidence: 56%

Effect of High Fat and High Fat-High Protein Diets on Biosynthesis of Niacin From Tryptophan in Rats

Shastri¹,

Nayudu²,

Nath³

1968

J. Vitaminol

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“…Simultaneous administration of sodium butyrate and tryptophan also resulted in hyperglycemia (17). It appears likely that the de crease in the synthesis of niacin from tryptophan, leading to decreased amounts of niacin in the blood and tissues may be responsible for some of the deleterious effects of the ketone bodies, reported earlier from this laboratory and confirmed by others (18,19 …”

Section: Fromsupporting

confidence: 69%

EFFECT OF ACETOACETATE AND Β-Hydroxy-Butyrate ON THE BIOSYNTHESIS OF NIACIN FROM TRYPTOPHAN

1967

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Effect of Infused Ketone Bodies on Glucose Utilization in the Dog*

Cited by 40 publications

References 19 publications

Long-term elevation of β-hydroxybutyrate in dairy cows through infusion: Effects on feed intake, milk production, and metabolism

Long-term elevation of β-hydroxybutyrate in dairy cows through infusion: Effects on feed intake, milk production, and metabolism

Effect of High Fat and High Fat-High Protein Diets on Biosynthesis of Niacin From Tryptophan in Rats

EFFECT OF ACETOACETATE AND Β-Hydroxy-Butyrate ON THE BIOSYNTHESIS OF NIACIN FROM TRYPTOPHAN

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