Effect of Ingested Culture of Propionibacterium freudenreichii ET-3 on Fecal Microflora and Stool Frequency in Healthy Females

Hojo, Kenichi; Yoda, Nobuo; Tsuchita, Hiroshi; Ohtsu, Tomoko; Seki, Keisuke; Taketomo, Naoki; Murayama, Tsutomu; Iino, Hisakazu

doi:10.12938/bifidus1996.21.115

Cited by 58 publications

(46 citation statements)

References 13 publications

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“…This property has been demonstrated both in vitro [110,111,178,179], and in vivo [127,[180][181][182] and the bifidogenic growth stimulators (BGS) involved in this effect were identified. The active compounds that were present in supernatants of P. freudenreichii, P. jensenii and P.…”

Section: Gut Microbial Modulationmentioning

confidence: 99%

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Dairy Propionibacteria: Less Conventional Probiotics to Improve the Human and Animal Health

Zárate¹

2012

Probiotic in Animals

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Section: Gut Microbial Modulationmentioning

confidence: 99%

“…The bifidogenic effect of selected strains of P. freudenreichii [127,[180][181][182] or purified BGS [184] was assessed in independent studies performed on human volunteers. As a general result, increased fecal bifidobacterial populations were observed even after some days after stopping the consumption of propionibacteria.…”

Section: Gut Microbial Modulationmentioning

confidence: 99%

Dairy Propionibacteria: Less Conventional Probiotics to Improve the Human and Animal Health

Zárate¹

2012

Probiotic in Animals

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“…However, it is not possible to make general statements on the consequences that EPS may have, since the EPS molecule's nature is highly diverse. P. freudenreichii is known as a probiotic bacterium mainly for its bifidogenic properties demonstrated in humans (31,53). Other health-promoting properties were described for the species (for a review, see reference 9).…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, some probiotic potentialities of dairy propionibacteria are well documented in human (for a review, see reference 9). Their most studied property is the bifidogenic effect (5,31,32,53). In a recent study (22), we investigated the immunomodulatory properties of 10 strains of dairy propionibacteria, based on the induction of regulatory anti-inflammatory cytokines in human peripheral blood mononuclear cells (PBMCs).…”

mentioning

confidence: 99%

Contribution of Surface β-Glucan Polysaccharide to Physicochemical and Immunomodulatory Properties of Propionibacterium freudenreichii

Deutsch

Parayre

Bouchoux

et al. 2012

Appl Environ Microbiol

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e Propionibacterium freudenreichii is a bacterial species found in Swiss-type cheeses and is also considered for its health properties. The main claimed effect is the bifidogenic property. Some strains were shown recently to display other interesting probiotic potentialities such as anti-inflammatory properties. About 30% of strains were shown to produce a surface exopolysaccharide (EPS) composed of (1¡3,1¡2)-␤-D-glucan due to a single gene named gtfF. We hypothesized that functional properties of P. freudenreichii strains, including their anti-inflammatory properties, could be linked to the presence of ␤-glucan. To evaluate this hypothesis, gtfF genes of three ␤-glucan-producing strains were disrupted. These knockout (KO) mutants were complemented with a plasmid harboring gtfF (KO-C mutants). The absence of ␤-glucan in KO mutants was verified by immunological detection and transmission electron microscopy. We observed by atomic force microscopy that the absence of ␤-glucan in the KO mutant dramatically changed the cell's topography. The capacity to adhere to polystyrene surface was increased for the KO mutants compared to wild-type (WT) strains. Antiinflammatory properties of WT strains and mutants were analyzed by stimulation of human peripheral blood mononuclear cells (PBMCs). A significant increase of the anti-inflammatory interleukin-10 cytokine production by PBMCs was measured in the KO mutants compared to WT strains. For one strain, the role of ␤-glucan in mice gut persistence was assessed, and no significant difference was observed between the WT strain and its KO mutant. Thus, ␤-glucan appears to partly hide the anti-inflammatory properties of P. freudenreichii; which is an important result for the selection of probiotic strains.

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“…GOS intake positively affects mouse intestinal flora, especially the amount of Bifidobacterium [14]. BGS has a similar intestinal regulatory effect [15,16]. The increase in the amount of Bifidobacterium in the intestine promotes the synthesis of lactic acid and acetic acid via the carbohydrate metabolism.…”

Section: Introductionmentioning

confidence: 99%

An Enteral Formula Containing Fermented Milk Products and Prebiotics Promotes Glucagon-Like Peptide-1 Secretion via Short Chain Fatty Acid Signaling

Kume¹,

Okazaki²,

Yamamoto³

et al. 2018

J Nutr Food Sci

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An enteral formula containing fermented milk products and prebiotics (prebiotic formula, PF) is known to promote the proliferation of Bifidobacterium and the production of short-chain fatty acids (SCFAs) in humans and rats. We studied the effect of PF on the secretion of glucagon-like peptide-1 (GLP-1) and the involvement of SCFAs in this process, using the knockout (KO) mice for the SCFA receptors G protein-coupled receptor 41 (GPR41) and G protein-coupled receptor 43 (GPR43). Wild type (WT) or KO mice were fed either a standard formula (SF) or PF for two weeks, and then were orally administered either PF or SF after overnight fasting and dissected after 0, 30, 60, and 240 minutes. Blood GLP-1 and glucose levels were measured (Experiment 1). Alternatively, mice fed SF or PF for two weeks were dissected after four hours of fasting, and their blood GLP-1 and cecal SCFAs levels were measured (Experiment 2). In Experiment 1, WT and GPR43KO mice showed a significant increase in GLP-1 concentration 30 and/or 60 minutes after formula administration in the PF group compared with that in the SF group. Similarly, WT and GPR43KO mice showed a significant suppression of the increase in glucose levels after formula administration in the PF group compared with that in the SF group. On the other hand, there was no significant difference in GLP-1 concentration or blood glucose levels between the two treatment groups in GPR41KO mice. In Experiment 2, there was a significant increase in cecal SCFA levels in the PF group compared with that in the SF group for all mice, as well as an increase in GLP-1 concentration. PF promotes GLP-1 secretion and SCFAs might contribute to the GLP-1 secretion that occurs directly after ingestion, through GPR41 signal transduction.

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Effect of Ingested Culture of Propionibacterium freudenreichii ET-3 on Fecal Microflora and Stool Frequency in Healthy Females

Cited by 58 publications

References 13 publications

Dairy Propionibacteria: Less Conventional Probiotics to Improve the Human and Animal Health

Dairy Propionibacteria: Less Conventional Probiotics to Improve the Human and Animal Health

Contribution of Surface β-Glucan Polysaccharide to Physicochemical and Immunomodulatory Properties of Propionibacterium freudenreichii

An Enteral Formula Containing Fermented Milk Products and Prebiotics Promotes Glucagon-Like Peptide-1 Secretion via Short Chain Fatty Acid Signaling

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