Effect of inhibin-like activity on LH-RH-stimulated release of FSH by pituitary glands from female rats in vitro

Jenner, A. A. J.; Koning, J. de; Rees, G. P. van

doi:10.1016/0024-3205(83)90114-5

Cited by 15 publications

(14 citation statements)

References 17 publications

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“…As pointed out in the Introduction, FSH release and synthesis consist of an LRH-dependent ('LHlike') component and an autonomous component which is inhibited by an ovarian protein, inhibin (De Jong et al 1978;Jenner et al 1982Jenner et al , 1983. So far no clear autonomous LH release and synthesis similar to that of FSH has been recognized.…”

Section: Discussionmentioning

confidence: 95%

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Divergent effects on LH and FSH synthesis and release from intact female rat pituitary glands in vitro by methylxanthines, cyclic AMP derivatives and sodium fluoride

Jenner¹,

Koning²,

Tijssen³

et al. 1985

Acta Endocrinologica

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FSH release from the female rat pituitary gland consists of an LH-like, LRH-dependent component and an autonomous, inhibin-sensitive component. It was investigated whether cyclic AMP mediated FSH release. BrcAMP, theophylline, MIX or NaF stimulated LH release but inhibited FSH release and synthesis. Although dbcAMP had no inhibitory effect on FSH release, it partly reversed the inhibitory action of theophylline. In view of previous and the present results it is concluded that cyclic AMP may mediate the LRHdependent LH and FSH release and, through a separate pathway, may mediate the inhibition of autonomous FSH release by the ovarian protein inhibin. Luteinizing hormone-releasing hormone (LRH) stimulates the release of the gonadotrophic hor¬ mones, luteinizing hormone (LH) and folliclestimulating hormone (FSH) from pituitary glands (Matsuo et al. 1971; Schally et al. 1971). Many reports deal with a possible role for cyclic AMP as an intracellular mediator for LRH-induced LH release, but only few of them also include FSH release (for review see Tixier-Vidal & Gourdji 1981; De Koning et al. 1981). Furthermore, secre¬ tion of FSH apparently is more complex than that of LH: FSH release consists of an autonomous (spontaneous or basal) component which can be inhibited by ovarian proteins (inhibin) and an LHlike, LRH-dependent component which is not af¬ fected by inhibin (Jenner et al. 1983). The present study was designed to investigate whether cyclic AMP mediates FSH release. For this purpose pitui¬ tary glands were incubated in the presence of agents that increase cyclic AMP levels. Materials and MethodsAdult female Wistar rats were decapitated on the second day of dioestrus. Two pituitary halves were placed in flasks each containing 1 ml medium TC 199 (Difco Labs.). The pre-incubation of 0.5 h and the successive 8 h incubation with fresh media were carried out at 37°C under continuous shaking and gassing with 02 and C02 (95%:5%). The following agents were dissolved in the media at final concentrations of 1 to 10 mM: 8-bromoadenosine-3':5'-mono-phosphoric acid (BrcAMP; Boeh¬ ringer), N6 -2' -0 -dibutyryl -adenosine -3',5'-monophos¬ phate (dbcAMP; Boehringer), l-methyl-3-isobutyl-xanthine (MIX; Sigma), NaF (Merck) and theophylline (DBH Chemicals Ltd.). The LH and FSH contents of the media and of saline-extracted pituitary glands were esti¬ mated by radioimmunoassay (Welschen et al. 1975;De Koning et al. 1982;Jenner et al. 1982). Since in one case (10 mM NaF treatment) pituitary wet weight was signifi¬ cantly higher, the results are expressed as µg LH-RP-1 or pg FSH-RP-1/pituitary tissue (mean ± SEM; = 4) instead of per mg pituitary wet weight (see e.g. De Koningetal. 1981Jenner et al. 1982Jenner et al. , 1983. The reference and iodination preparations were generous Table 1. ). Statistical comparisons were made by analysis of variance followed by Duncan's mul¬ tiple comparison test (Steel 8c Torrie 1960). Results Experiment 1The effects of 0, 1 and 10 mM dbcAMP in the presence and absence of 10 mM theophylline were stu...

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Section: Discussionmentioning

confidence: 95%

“…1981Koningetal. , 1982Jenner et al 1982Jenner et al , 1983. The reference and iodination preparations were generous The effect of dbcAMP and/or theophylline on FSH release and total FSH content (i.e.…”

mentioning

confidence: 99%

Divergent effects on LH and FSH synthesis and release from intact female rat pituitary glands in vitro by methylxanthines, cyclic AMP derivatives and sodium fluoride

Jenner¹,

Koning²,

Tijssen³

et al. 1985

Acta Endocrinologica

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“…There are indications that once FSH synthesis has started, spontaneous release by the pituitary may occur without LHRH stimulation. In particular, this part of FSH secretion is under the control of negative feedback through inhibin (24). In addition to this autonomous FSH secretion, pulsatile FSH secretion occurs in association with exogenous or endogenous LHRH pulses (25,26), but the link between LHRH and FSH pulses is much less than between LHRH and LH pulses.…”

Section: Discussionmentioning

confidence: 99%

Increased Levels and Pulsatility of Follicle-Stimulating Hormone in Mothers of Hereditary Dizygotic Twins¹

Lambalk¹,

Boomsma

Boer³

et al. 1998

The Journal of Clinical Endocrinology & Metabolism

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“…Inhibin is a gonadal protein, presumably produced by the granulosa cells of develop ing follicles and liberated into the follicular fluid [21,22], After interaction with the pitu itary gonadotrope [23,24], IBN induces a relatively selective suppression of FSH re lease [1][2][3][4], Physiologically the second phase of FSH release, which is not paralleled by an increase in LH, results from gonadotrope de repression owing to a decrease in IBN nega tive feedback and initiates the next wave of folliculogenesis [4], However, some authors have reported a dampening of the LH re sponse to GnRH following IBN administra tion [25,26], consistent with the observed IBN-induced suppression of GnRH-induced LH release in vitro reported here.…”

Section: Discussionmentioning

confidence: 99%

“…Follicle-stimulating hormone (FSH) se cretion is under both positive and negative control; while gonadotropin-releasing hor mone (GnRH) stimulates FSH release, the GnRH-induced FSH response can be blocked by the gonadal protein, inhibin (IBN) [1,2], The suppressive effects of IBN have been reported to be selective in that FSH release is reduced, while luteinizing hormone (LH) release is relatively unaltered [3,4], As the second messenger for FSH remains unclear, the mechanism underlying such selectivity is still a mystery. It has be come evident that calcium plays an integral role in the transduction of LH release follow ing GnRH binding at the pituitary gonadotrope [5].…”

Section: Introductionmentioning

confidence: 99%

Cyclic Nucleotides and Suppression of Follicle-Stimulating Hormone Release by Inhibin

Simpson

Vernon²,

Maley³

et al. 1987

Gynecol Obstet Invest

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The relative roles of the cyclic nucleotide messengers cAMP and cGMP in the suppression of follicle-stimulating hormone (FSH) secretion in response to inhibin (IBN) were assessed employing rat gonadotropes in monolayer culture. While exposure of cells to gonadotropin-releasing hormone (GnRH) induced a significant increase in the amounts of both FSH and luteinizing hormone (LH) released into the culture medium, these responses were dampened by the administration of IBN (in porcine follicular fluid). Addition of cGMP to the system failed to restore FSH release, while cAMP restored basal FSH release. Modulation of nucleotide metabolism with theophylline, sodium nitroprusside, and a protein kinase inhibitor failed to overcome the IBN-induced suppression of FSH release. The cellular content of calmodulin increased in response to GnRH, a response antagonized by IBN. Cellular levels of cGMP were also increased by GnRH, but this response was unaltered by IBN. The administered drugs all failed to reverse these effects of IBN. These data indicate that the IBN-induced suppression of FSH release is not dependent upon the cyclic nucleotides cAMP and/or cGMP. However, a role in the maintenance of basal FSH synthesis and release for cAMP is indicated.

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Effect of inhibin-like activity on LH-RH-stimulated release of FSH by pituitary glands from female rats in vitro

Cited by 15 publications

References 17 publications

Divergent effects on LH and FSH synthesis and release from intact female rat pituitary glands in vitro by methylxanthines, cyclic AMP derivatives and sodium fluoride

Divergent effects on LH and FSH synthesis and release from intact female rat pituitary glands in vitro by methylxanthines, cyclic AMP derivatives and sodium fluoride

Increased Levels and Pulsatility of Follicle-Stimulating Hormone in Mothers of Hereditary Dizygotic Twins¹

Cyclic Nucleotides and Suppression of Follicle-Stimulating Hormone Release by Inhibin

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Effect of inhibin-like activity on LH-RH-stimulated release of FSH by pituitary glands from female rats in vitro

Cited by 15 publications

References 17 publications

Divergent effects on LH and FSH synthesis and release from intact female rat pituitary glands in vitro by methylxanthines, cyclic AMP derivatives and sodium fluoride

Divergent effects on LH and FSH synthesis and release from intact female rat pituitary glands in vitro by methylxanthines, cyclic AMP derivatives and sodium fluoride

Increased Levels and Pulsatility of Follicle-Stimulating Hormone in Mothers of Hereditary Dizygotic Twins1

Cyclic Nucleotides and Suppression of Follicle-Stimulating Hormone Release by Inhibin

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Increased Levels and Pulsatility of Follicle-Stimulating Hormone in Mothers of Hereditary Dizygotic Twins¹