Effect of inhibition of protein synthesis on lipid metabolism in Lactobacillus plantarum

Arbogast, Loretta Y.; Henderson, Thomas O.

doi:10.1128/jb.123.3.962-971.1975

Cited by 13 publications

(11 citation statements)

References 23 publications

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“…The crude fat content also showed a marginal increase in LPFT and BLFT (4% and 7.3% respectively), which can be attributed to the production of fatty acids by the bacterial strains. The increase in the crude fat content could be correlated with the increase in the protein content as reported by Arbogast and Henderson (1975), in that the polar lipid synthesis is associated with the protein synthesis because the enzymes required for the lipid synthesis are to be replaced by de novo protein synthesis. As such, fish wastes have a very low fibre content and it requires no further discussion, for the low crude fibre content in both LPFT and BLFT.…”

Section: Resultssupporting

confidence: 52%

Biotransformation of tuna waste by co-fermentation into an aquafeed ingredient

Vijayan

Imelda

Raj

2009

Aquaculture Research

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Dried skipjack tuna (Katsuwonus pelamis) waste (red meat, gills, viscera, ¢ns, etc.) ). Changes in the nutritional quality (crude protein, crude fat, crude ash, crude ¢bre and nitrogen-free extract and aminoacids) were monitored during a fermentation period of14 days. The proximate analysis showed signi¢cant changes in the composition of L. plantarum-fermented tuna (LPFT) and B. licheniformis-fermented tuna (BLFT) from the unfermented raw materials. Fermentation of tuna waste has resulted in a signi¢cant (Po0.05) increase in the protein content of tuna waste between days 6 and12. All the amino acid contents in BLFT increased during fermentation, whereas, in LPFT the levels of serine, histidine, tyrosine, methionine, cystine and phenylalanine contents were decreased. A marginal increase in calcium and phosphorus levels was recorded in the fermented products. The results of the study suggest that LPFT or BLFT can be used as a novel aquafeed ingredient for di¡erent ¢sh species.

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Section: Resultssupporting

confidence: 52%

Biotransformation of tuna waste by co-fermentation into an aquafeed ingredient

Vijayan

Imelda

Raj

2009

Aquaculture Research

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“…An analysis of residual divisions after the initiation of antibiotic treatment indicated that cerulenin affected an event about 43 min before the cessation of division and approximately 10 min before the termination of chromosomal replication as determined by treatment of similar cultures with mitomycin C. Transient increases in lipid synthesis have been reported in the late stages of the cell division cycle in bacteria (6,11). Also, observations in many other bacterial species (1,9,10,13,16,20) are consistent with a primary lipid involvement in the cell division process. The present study suggests that cerulenin, which blocks de novo lipid and LTA synthesis, can block a crucial cell division event.…”

Section: Resultsmentioning

confidence: 93%

Effect of cerulenin on Streptococcus faecalis macromolecular synthesis and cell division

Carson¹,

Daneo-Moore²

1978

J Bacteriol

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The antibiotic cerulenin has been used to study macromolecular synthesis and cell division in Streptococcus faecalis. The data suggest that lipid and lipoteichoic acid synthesis as well as cell number increase are affected prior to any observable effects on overall mass increase or DNA, RNA, protein, or peptidoglycan synthesis. Treatment with cerulenin of cultures growing at various rates and analysis of the subsequent cell divisions indicate that the antibiotic may block a cell cycle event that precedes the completion of chromosome replication by about 10 mmn Cleveland et al. (4,5) have shown that autolytic activity in Streptococcus faecalis is inhibited both in cell-free extracts and in intact cells by lipoteichoic acid (LTA) as well as by certain native lipids. This suggests that certain lipids and/or LTA may function in the directional regulation of wall synthesis necessary for septum formation during the normal growth and division cycle of these streptococci (7,8). It has been proposed by Shockman et al. (15) that such regulation would occur through inhibition ofautolytic activity associated with chromosome termination, i.e., during the final 25 to 30 min before division of S. faecalis.We have employed the antibiotic cerulenin, an inhibitor of fatty acid synthesis in bacteria (19), to block the synthesis of lipids and LTA in S. faecalis ATCC 9790 in an effort to delineate their functions in the divisional process of these bacteria. MATERIALS AND METHODSGrowth ofcells. S. faecalis ATCC 9790 was grown in a chemically defined medium (14) at 37°C. Culture turbidity was followed spectrophotometrically on a Coleman spectrophotometer model 14 at 675 nm. The absorbance was corrected for deviations from linearity (18), yielding adjusted optical density units (AOD). Cultures were maintained in balanced growth for at least 8 to 10 mass doublings before an experiment.Determination of cell numbers. Culture samples (0.1 ml) were fixed for 30 min on ice in 0.4 ml of 7.4% formaldehyde in distilled water. Dilutions for counting were made with 0.9% NaCl that had been previously filtered through a nitrocellulose filter (0.22-pum pore size, 47-mm diameter, Millipore Corp., Bedford, Mass.). Cell numbers were obtained with an Electrozone/Celloscope particle counter (30-pm-diameter orifice; Particle Data Corp., Elmhurst, ill.). Determination of macromolecular synthesis. Cultures were grown for at least five to seven generations in the presence of [14C]or [3H]glycerol (0.5 ,uCi/ml, 8 pg/ml), [14C]thymidine (1 pCi/ml, 15 pug/ml),[3H"lysine (0.5 pCi/ml, 30 pg/ml), or [14Cluracil (0.5 pCi/ml, 30 pug/ml) before sampling. Isotopes were purchased from New England Nuclear Corp., Boston, Mass. Total trichloroacetic acid-precipitable isotope incorporation was determined by placing 0.5-ml samples in 4.5 ml of 10% trichloroacetic acid on ice for a minimum of 30 min, filtering through a Reeve-Angel 984-H glas-fiber filter (Hurlbert Paper Co., South Lee, Mass.), and washing the filters twice with 2 ml of ice-cold 10% trichloroacetic acid and o...

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“…(2005) reported that in LAB, the citric acid once inside the cell is split into acetate and oxaloacetate by the citrate lyase enzyme. The acetate formed can be expelled or part of it can be used in biosynthetic reactions, such as fatty acid synthesis (Arbogast and Henderson 1975; Goldberg and Eschar 1977). These facts could explain the decrease in acetic acid concentrations observed in CDM without Cit.…”

Section: Discussionmentioning

confidence: 99%

Effect of biosynthetic intermediates and citrate on the phenyllactic and hydroxyphenyllactic acids production by Lactobacillus plantarum CRL 778

Dallagnol

Catalán

Mercado

et al. 2011

Journal of Applied Microbiology

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Aim: To evaluate the influence of biosynthetic precursors, intermediates and electron acceptors on the production of antifungal compounds [phenyllactic acid (PLA) and hydroxyphenyllactic acid (OH‐PLA)] by Lactobacillus plantarum CRL 778, a strain isolated from home‐made sourdough. Methods and Results: Growth of fermentative activity and antifungal compounds production by Lact. plantarum CRL 778 were evaluated in a chemically defined medium (CDM) supplemented with biosynthetic precursors [phenylalanine (Phe), tyrosine (Tyr)], intermediates [glutamate (Glu), alpha‐ketoglutarate (α‐KG)] and electron acceptors [citrate (Cit)]. Results showed that the highest PLA production (0·26 mmol l−1), the main antifungal compound produced by Lact. plantarum CRL 778, occurred when greater concentrations of Phe than Tyr were present. Both PLA and OH‐PLA yields were increased 2‐folds when Cit was combined with α‐KG instead of Glu at similar Tyr/Phe molar ratio. Similarly, glutamate dehydrogenase (GDH) activity was significantly (P < 0·01) stimulated by α‐KG and Cit in Glu‐free medium. Conclusion: Phe was the major stimulant for PLA formation; however, Cit could increase both PLA and OH‐PLA synthesis by Lact. plantarum CRL 778 probably due to an increase in oxidized NAD+. This effect, as well as the GDH activity, was enhanced by α‐KG and down regulated by Glu. Significance and Impact of the Study: This is the first study where the role of Glu and GDH activity in the PLA and OH‐PLA synthesis was evidenced in sourdough lactic acid bacteria (LAB) using a CDM. These results contribute to the knowledge on the antifungal compounds production by sourdough LAB with potential applications on the baked goods.

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Effect of inhibition of protein synthesis on lipid metabolism in Lactobacillus plantarum

Abstract: In Lactobacillus plantarum 17-5, lipid synthesis appears to be correlated with protein synthesis. Inhibition of protein synthesis by chloramphenicol (50,ug/ml)

Cited by 13 publications

References 23 publications

Biotransformation of tuna waste by co-fermentation into an aquafeed ingredient

Biotransformation of tuna waste by co-fermentation into an aquafeed ingredient

Effect of cerulenin on Streptococcus faecalis macromolecular synthesis and cell division

Effect of biosynthetic intermediates and citrate on the phenyllactic and hydroxyphenyllactic acids production by Lactobacillus plantarum CRL 778

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