Effect of Initial Freezing Temperature, Addition of Glycerol and Dilution on the Survival and Fertilizing Ability of Deep-Frozen Ram Semen

Colas, G.

doi:10.1530/jrf.0.0420277

Cited by 98 publications

(44 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, in vitro tests done on semen are not always related to the fertility of semen in vivo (Colas 1975;Tardif et al 1999). It is possible that the impact of glycerol is within the female genital tract.…”

Section: Effect Of Glycerol On Fresh Semenmentioning

confidence: 99%

“…Colas (1975) suggested that glycerol is slightly toxic to ram spermatozoa and that its negative effects could be reduced by its addition at a temperature near 0°C. Contrary to this recommendation, Salomon (1968) [reviewed by Salomon and Maxwell (2000)] reported that a single dilution of semen with an extender containing glycerol was as effective as a two-step dilution.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Glycerol addition and conservation of fresh and cryopreserved ram spermatozoa

Morrier¹,

Castonguay²,

Bailey³

2002

Can. J. Anim. Sci.

View full text Add to dashboard Cite

. 2002. Glycerol addition and conservation of fresh and cryopreserved ram spermatozoa. Can. J. Anim. Sci. 82: 347-356. Fresh extended ram semen has a short fertile lifespan whereas acceptable fertility with cryopreserved semen is achieved only by laparoscopy, which limits widespread artificial insemination in sheep. Although glycerol is considered essential for freezing spermatozoa, it is often included in extenders for short-term storage at above-freezing temperatures. To test the hypothesis that glycerol reduces the function of fresh sperm, ram semen was divided into two aliquots and diluted with commercial extenders that were identical, except that one contained 7% glycerol (n = 6). In a second experiment, ram semen was prepared for cryopreservation by a one-step dilution with a 7% glycerol extender or gradually, with a two-step protocol, to test the hypothesis that the method and time of glycerol addition affects sperm quality after freezing and thawing (n = 7). For both experiments, semen was diluted in a synthetic oviductal fluid (SOF-m) and sperm quality was assessed by computerassisted motility, viability and chlortetracycline fluorescence (CTC) patterns (an indicator of capacitation status). The presence of glycerol did not affect the quality of fresh sperm (P > 0.27). For cryopreserved sperm, the method of glycerol addition also did not affect thawed sperm. However, a decrease in sperm motility and viability, and different distribution of CTC patterns occurred due to the duration of time in extender and in SOF-m (P ≤ 0.0002). Cryo-capacitation was also observed. In conclusion, the presence of glycerol in the extender did not reduce ram sperm quality during conservation of the semen at 5°C or when it was used to completely and rapidly dilute the semen before cooling for cryopreservation. La semence de bélier fraîche (conservée à 5°C) a une durée de vie limitée tandis que la semence cryoconservée ne donne des taux de fertilité acceptables que lorsque la laparoscopie est utilisée, ce qui limite l'expansion de l'insémination artificielle chez l'ovin. Même si le glycérol est essentiel à la congélation des spermatozoïdes, il est souvent inclus dans les diluants destinés à la conservation de courte durée à des températures au-dessus du point de congélation. Afin de tester l'hypothèse selon laquelle le glycérol réduit la fonction des spermatozoïdes frais, la semence de bélier a été divisée en deux aliquotes et diluée avec deux diluants commerciaux identiques, à l'exception du fait qu'un contenait 7% de glycérol (n = 6). Dans une seconde expérience, la semence de bélier était diluée en une seule étape avec un diluant contenant 7% de glycérol ou graduellement, en deux étapes afin de tester l'hypothèse selon laquelle la méthode et le temps d'addition du glycérol affectent la qualité spermatique lors de la congélation-décongélation (n = 7). Dans les deux expériences, la semence était diluée dans un fluide synthétique mimant l'oviducte de la brebis (SOF-m) et la qualité de la semence était évaluée selon la motilité...

show abstract

Section: Effect Of Glycerol On Fresh Semenmentioning

confidence: 99%

mentioning

confidence: 99%

Glycerol addition and conservation of fresh and cryopreserved ram spermatozoa

Morrier¹,

Castonguay²,

Bailey³

2002

Can. J. Anim. Sci.

View full text Add to dashboard Cite

show abstract

“…Glycerol has been widely utilized as a cryoprotectant for frozen storage of spermatozoa of several mammals, including bull (Polge & Rowson, 1952), a dog (Seager, 1969), sheep (Colas, 1975), horse (Nishikawa et al, 1968) and pig (Pursel & Johnson, 1975 Graham et al, 1978). In that study, mouse epididymal spermatozoa were suspended in a skim milk medium (10-5% solids) containing 0-75% fructose, 1% bovine serum albumin and 4mM-calcium chloride and then frozen.…”

Section: Discussionmentioning

confidence: 99%

“…Since Polge & Rowson (1952) first reported that glycerol could provide considerable protection to bull spermatozoa during freezing and thawing, there have been many reports on the cryopreservation of spermatozoa of various species including horse (Nishikawa et al, 1968), pig (Pursel & Johnson, 1975), sheep (Colas, 1975), dog (Seager, 1969), rabbit (Fox, 1961), mouse (Graham et al, 1978, review), and man (Bunge & Sherman, 1953). Such reports are limited to relatively large animals;…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation of mouse spermatozoa in the presence of raffinose and glycerol

Tada¹,

Sato²,

Yamanoi³

et al. 1990

Reproduction

188

View full text Add to dashboard Cite

“…Ejaculated and epididymal sperm samples stored at 4°C were cryopreserved in 0.25 ml straws according to method of Colas (Colas, 1975). For both species, sperm was cryopreserved with skim milk diluent at a concentration of 100 × 10 6 spz/straw for Ile de France rams and 60 × 10 6 spz for Corriedale rams.…”

Section: Fernández Abella Da Costa Guérin and Dacheuxmentioning

confidence: 99%

Fertility of undiluted ram epididymal spermatozoa stored for several days at 4°C

Abella

Costa

Guérin

et al. 2015

Animal

View full text Add to dashboard Cite

In vitro preservation of the male gamete is a challenge in the development of artificial insemination techniques for domestic animals. Specific strategies and diluents have been developed for the preservation of the fertilizing ability of the semen for each species. However, the epididymal medium has been demonstrated to be the best sperm environment to maintain sperm viability over several days and weeks for mammals. The aims of this study were to evaluate the motility and in vivo fertility of ram epididymal spermatozoa when the semen was stored for up to 4 days at 4°C undiluted in epididymal plasma. The study was undertaken with two ovine breeds (Ile de France and Corriedale). The motility of epididymal spermatozoa was better preserved in the undiluted epididymal fluid than when epididymal spermatozoa were diluted in classic ovine extender such as skim milk. During storage, the decrease in the percentage of motile sperm was lower if the epididymal spermatozoa were collected immediately after epididymal sampling than 24 h after castration or animal death. The fertility obtained after cryopreservation of the stored sperm and subsequent intrauterine insemination ranged from 55% to 24% following 24 to 96-h sperm storage. There was a linear regression relationship between fertility and the number of motile sperm inseminated for both breeds. These results show that it is possible to keep epididymal sperm motile and fertile for several days without dilution. Such a method of sperm preservation could be a final possibility for animals of high genetic value or for endangered species when the collection of semen before death of the animal is not possible.

show abstract

Effect of Initial Freezing Temperature, Addition of Glycerol and Dilution on the Survival and Fertilizing Ability of Deep-Frozen Ram Semen

Cited by 98 publications

References 9 publications

Glycerol addition and conservation of fresh and cryopreserved ram spermatozoa

Glycerol addition and conservation of fresh and cryopreserved ram spermatozoa

Cryopreservation of mouse spermatozoa in the presence of raffinose and glycerol

Fertility of undiluted ram epididymal spermatozoa stored for several days at 4°C

Contact Info

Product

Resources

About