Effect of inocula on heavy metal removal by sulfate-reducing columns

Messner, N.; Reardon, Kenneth F.; Pruden, Amy; Pereyra, Luciana P.; Maz√≥n, C Sans

doi:10.1201/9780203021637.ch25

Cited by 1 publication

(4 citation statements)

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“…This study demonstrates the influence of microbial inoculum in the removal of sulfates and metals from AMD and supports our previous findings in column experiments (Messner, 2004). Bottles inoculated with LUTR inoculum collected from a field PRZ showed the best overall performance and demonstrated the highest metal removal.…”

Section: Discussionsupporting

confidence: 90%

“…Five different inocula were used for this study: twice-acclimated inoculum from a sulfatereducing column simulating a PRZ (Messner et al, 2004), dairy manure, anaerobic digester sludge, and inocula taken from two sulfate-reducing bioreactors operated in the field (Luttrell and Peerless Jenny King, near Helena, MT, kindly provided by Jim Gusek of Golder Associates, Inc.). Anaerobic digester sludge samples were obtained from the Drake Water Reclamation Facility of the city of Fort Collins, CO. Fresh dairy manure was obtained from La Luna dairy, Wellington, CO.…”

Section: Candidate Inoculamentioning

confidence: 99%

“…The bottles were prepared in an anaerobic hood under a nitrogen atmosphere. The simulated mine water was prepared by adding 1.3204 g/L MgSO 4 ; 0.0268 g/L NH 4 Cl; 0.088 g/L ZnSO 4 .7H 2 O; 0.0623 g/L FeSO 4 .7H 2 O and 0.0088 g/L CdCl 2 to deionized water purged with nitrogen (Messner et al, 2004). Because this study focused on the microbiology, the ammonium was added to ensure that there were no limiting factors for microbial growth.…”

Section: Batch Experiments Set Upmentioning

confidence: 99%

“…Our past research has demonstrated the controlling influence of microbial inoculum on the performance of sulfate-reducing columns simulating PRZs (Messner et al, 2004). Columns were inoculated with an acclimated inoculum, dairy manure and no inoculum and their performance was evaluated according to their capacity to remove metals, sulfates and to neutralize the pH.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of Inocula Applied in the Remediation of Acid Mine Drainage by Sulfate Reduction

Pereyra¹,

Hanson²,

Hiibel³

et al. 2005

ASMR

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Sulfate-reducing permeable reactive zones (PRZs), such as anaerobic wetlands, sulfate-reducing bioreactors, and permeable reactive barriers, are an attractive means of passively treating mining influenced waters contaminated with heavy metals. While the low cost and maintenance requirements are significant advantages of PRZs, the lack of clear design criteria is a disadvantage. It is not known why some systems will function for long periods of time without need for intervention, while others fail or do not recover well when exposed to stresses such as winter weather or other changes in conditions. This study explores the role of microorganisms in PRZs and the potential to use selected inocula to improve performance with respect to start-up time, sulfate-reducing activity level, and activity retention time. We have compared these attributes using various inocula, including: dairy manure, anaerobic digester sludge, acclimated column inoculum, and inoculum collected from two sulfate-reducing bioreactors operated in the field (Luttrell and Peerless Jenny King). Our results demonstrate that there are clear differences between the inocula and that the Luttrell bioreactor inoculum performs the best in terms of start-up time and overall activity. Sulfate concentrations, metal concentrations, and pH were measured in the aqueous phase to evaluate the ability of the different inocula to remediate acid mine drainage (AMD). In subsequent studies, DNA-based methods that profile the microbial community will be used to determine what kinds of microorganisms are present and to quantify key functional groups, including sulfate reducers, methanogens, and cellulose degraders. The ultimate goal will be to transfer these results to the field by developing the capability to intelligently design inocula for site-specific concerns.

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Section: Discussionsupporting

confidence: 90%

Section: Candidate Inoculamentioning

confidence: 99%

Section: Batch Experiments Set Upmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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