Effect of Inoculation Process on Lycopene Production by Blakeslea trispora in a Stirred-Tank Reactor

Wang, Qiang; Li, Feng; Wei, Luo; Li, Hanguang; Zhou, Yecheng; Yu, Xiaobin

doi:10.1007/s12010-014-1327-y

Cited by 19 publications

(4 citation statements)

References 31 publications

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“…The quality of the seed culture has a great influence on the fermentation result (Wang et al 2015 ). Generally, inoculation with a seed culture in the logarithmic growth phase has strong advantages for cellular metabolism and growth, which effectively shortens the lag phase, increasing the product yield (Ji et al 2009 ).…”

Section: Resultsmentioning

confidence: 99%

RETRACTED ARTICLE: Mutagenesis combined with fermentation optimization to enhance gibberellic acid GA3 yield in Fusarium fujikuroi

Yang

Peng

et al. 2022

Bioresour. Bioprocess.

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Gibberellic acid (GA3) is a plant growth hormone that plays an important role in the production of crops, fruits, and vegetables with a wide market share. Due to intrinsic advantages, liquid fermentation of Fusarium fujikuroi has become the sole method for industrial GA3 production, but the broader application of GA3 is hindered by low titer. In this study, we combined atmospheric and room-temperature plasma (ARTP) with ketoconazole-based screening to obtain the mutant strain 3-6-1 with high yield of GA3. Subsequently, the medium composition and fermentation parameters were systematically optimized to increase the titer of GA3, resulting in a 2.5-fold increase compared with the titer obtained under the initial conditions. Finally, considering that the strain is prone to substrate inhibition and glucose repression, a new strategy of fed-batch fermentation was adopted to increase the titer of GA3 to 575.13 mg/L, which was 13.86% higher than the control. The strategy of random mutagenesis combined with selection and fermentation optimization developed in this study provides a basis for subsequent research on the industrial production of GA3. Graphical Abstract

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Section: Resultsmentioning

confidence: 99%

RETRACTED ARTICLE: Mutagenesis combined with fermentation optimization to enhance gibberellic acid GA3 yield in Fusarium fujikuroi

Yang

Peng

et al. 2022

Bioresour. Bioprocess.

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“…Lycopene production remained nearly unchanged upon magnetic field treatment on the first day of fermentation, while a substantial increase was observed on the second and third days. This can be attributed to the primary production of lycopene during the microorganisms' stable growth phase [16]. The highest lycopene production was achieved after 48 h of exposure, followed by a gradual decline in production with longer treatment durations compared to the control group.…”

Section: Magnetic Field Processing Timementioning

confidence: 95%

“…The extracted solution was filtered and diluted, and its absorbance was measured at a wavelength of 502 nm. Lycopene content was quantified using a standard curve (Figure S1) of lycopene [16].…”

Section: Detection Of Biomass and Lycopene Yieldmentioning

confidence: 99%

Enhancement of Lycopene Synthesis via Low-Frequency Alternating Magnetic Field in Brassica trispora

Wang,

Hou,

Wang

et al. 2024

Fermentation

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In recent years, magnetic fields have emerged as a non-thermophysical treatment with a significant impact on microbial fermentation processes. Brassica trispora is a microorganism known for its industrial-scale production of lycopene and high yield of single cells. This study aimed to investigate the impact of low-frequency magnetic fields on lycopene synthesis by Brassica trispora and elucidate the underlying mechanism for enhancing lycopene yield. The results indicate that both the intensity and duration of the magnetic field treatment influenced the cells. Exposing the cells to a 0.5 mT magnetic field for 48 h on the second day of fermentation resulted in a lycopene yield of 25.36 mg/g, representing a remarkable increase of 244.6% compared to the control group. Transcriptome analysis revealed that the alternating magnetic field significantly upregulated genes related to ROS and the cell membrane structure, leading to a substantial increase in lycopene production. Scanning electron microscopy revealed that the magnetic field treatment resulted in a rough, loose, and wrinkled surface morphology of the mycelium, along with a few micropores, thereby altering the cell membrane permeability to some extent. Moreover, there was a significant increase in intracellular ROS content, cell membrane permeability, key enzyme activity involved in lycopene metabolism, and ROS-related enzyme activity. In conclusion, the alternating frequency magnetic field can activate a self-protective mechanism that enhances lycopene synthesis by modulating intracellular ROS content and the cell membrane structure. These findings not only deepen our understanding of the impact of magnetic fields on microbial growth and metabolism but also provide valuable insights for developing innovative approaches to enhance carotenoid fermentation.

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“…1,5 Currently, microbial production of lycopene employed in industrialized processes uses the natural carotenogenic microorganism Blakeslea trispora. 6,7 However, the production of lycopene from B. trispora requires the addition of cyclase inhibitors, which are expensive and cause food safety issues. 8 Additionally, genetic manipulation is not easy in B. trispora, thereby hindering its metabolic engineering for yield improvement.…”

Section: ■ Introductionmentioning

confidence: 99%

Metabolic Redesign of Rhodobacter sphaeroides for Lycopene Production

Chi

Liu

et al. 2018

J. Agric. Food Chem.

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Lycopene plays an important role as an antioxidative and anticancer agent, and is an increasingly valuable commodity in the global market. Rhodobacter sphaeroides, a carotenogenic and phototrophic bacterium, is an efficient and practical host for carotenoid production. Herein, we explored the potential of metabolically engineered Rb. sphaeroides as a novel platform to produce lycopene. The basal lycopene-producing strain was generated by introducing an exogenous crtI from Rhodospirillum rubrum to replace the native crtI and deleting crtC in Rb. sphaeroides. Furthermore, knocking out zwf blocked the competitive pentose phosphate pathway and improved the lycopene content by 88%. Finally, the methylerythritol phosphate pathway was reinforced by integration of dxs combined with zwf deletion, which further increased the lycopene content. The final engineered strain produced lycopene to 10.32 mg/g dry cell weight. This study describes a new lycopene producer and provides insight into a photosynthetic bacterium as a host for lycopene biosynthesis.

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Effect of Inoculation Process on Lycopene Production by Blakeslea trispora in a Stirred-Tank Reactor

Cited by 19 publications

References 31 publications

RETRACTED ARTICLE: Mutagenesis combined with fermentation optimization to enhance gibberellic acid GA3 yield in Fusarium fujikuroi

RETRACTED ARTICLE: Mutagenesis combined with fermentation optimization to enhance gibberellic acid GA3 yield in Fusarium fujikuroi

Enhancement of Lycopene Synthesis via Low-Frequency Alternating Magnetic Field in Brassica trispora

Metabolic Redesign of Rhodobacter sphaeroides for Lycopene Production

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