Effect of inoculum source, electron donors, and substrates on medium-chain fatty acid production from palm oil mill effluent

Kurniawan, Edy; Gagnon, Yves; Cheirsilp, Benjamas; O-Thong, Sompong

doi:10.1016/j.indcrop.2023.117654

Cited by 6 publications

(1 citation statement)

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“…It can also be produced synthetically from petroleum. Caproic acid is also produced by chain elongation in anaerobic fermentation by chain-elongating bacteria, including Clostridium kluyveri [1,2] and Megasphaera elsdenii [3,4], Caproiciproducens [5,6], and Ruminococcaceae bacterium [7]. Caproic acid is synthesized by the reverse β-oxidation of short-chain fatty acids (e.g., acetic acid C 2 , propionic acid C 3 , and butyric acid).…”

Section: Introductionmentioning

confidence: 99%

Caproic acid production through fermentation with a thermotolerant Clostridium strain M1NH

Kurniawan,

O-Thong,

Imai

2023

Preprint

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Thermotolerant caproic acid-producing bacteria were isolated from sewage sludge and evaluated for caproic acid production with various substrates. The ethanol-acetate was the most efficient substrate for enriched culture, with 2.7 g/L caproic acid and a yield of 47%. Clostridium strain M1NH was isolated from enriched culture and identified as a gram-positive, rod-shaped, spore-forming with 92% similarity of 16S rRNA to Clostridium kluyveri. Clostridium M1NH has an optimal pH and temperature of 6-6.5 and 35–40°C with a growth rate of 0.11–0.14 h-1, suggesting that the strain was thermotolerant. Clostridium strain M1NH displays the highest caproic acid production of 3.2–3.5 g/L at a pH of 6.5, a temperature of 35–40°C, and an ethanol-acetate molarity ratio of 4:1. Clostridium strain M1NH is tolerant to concentrations of ethanol, acetate, and caproic acid of 5%, 5%, and 2%, respectively. The thermotolerant Clostridium M1NH presents a significant advancement in harnessing sustainable biobased production processes, holding great promise for applications in thermotolerant fermentation.

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Section: Introductionmentioning

confidence: 99%