Effect of Input Multiplicity and Tissue Cell Concentration Upon the Growth of Rift Valley Fever Virus

Abstract

The effects of virus input multiplicity and of tissue cell concentration upon the growth of Rift Valley fever virus in L cells (Earle) were determined. The titers obtained in suspension cultures with cells obtained from two separate laboratories were significantly different. With both monolayer culture and suspension culture systems, a virus input multiplicity of 2.5 resulted in the greatest proliferation of virus. Optimal viral yields were obtained in suspension cultures containing 4 X 105 tissue cells per ml of suspension.

Evaluation of Factors Related to Growth of Rift Valley Fever Virus in Suspended Cell Cultures

“…15 we suggest that the ability of low MO to reach the same peak titer may be attributable to the fact that cells and virus in suspensions are constantly in motion and consequently have multiple contacts and possibilities of cell attachment, but movement of virus in monolayer system is e6enrially Brownian motion. he lack of cell-associated virus in suspension culture indicates thai, the virus is released to the medium soon after its formation within the tissue cell.…”

Evaluation of Factors Related to Growth of Rift Valley Fever Virus in Suspended Cell Cultures

“…Orlando et al (12), of these laboratories, reported that the optimal virus input multiplicity was approximately 2.5. More recently, Johnson et al (9), using a monolayer tissue culture system of mouse fibroblast-like cells, described the growth of RVFV and reported on optimal virus input multiplicities and medium volume for this system.…”