Effect of inserted spacer in hepatic cell-penetrating multifunctional peptide component on the DNA intracellular delivery of quaternary complexes based on modular design

Zhang, Luchen; Li, Zhenbo; Sun, Fangli; Xu, Yuhong; Du, Zongliang

doi:10.2147/ijn.s115381

Cited by 8 publications

(2 citation statements)

References 23 publications

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“…Circular dichroism (CD) spectra were used to investigate the conformational change of Herceptin in Herceptin‐HA, indicating that the crosslink of Herceptin and HA led to a limited conformational change of Herceptin in Herceptin‐HA (Figure 1e). HA had a broad negative peak at 210 nm, [ 30 ] while Herceptin had a positive peak at 205 nm and a negative peak at 217 nm, reflecting an immunoglobin β ‐sheet fold of normal Herceptin. [ 15 ] The absorption peak of Herceptin+HA matched closely to the simple superposition of the absorption peak of free HA and Herceptin, indicating that the Herceptin domain maintained a standard folded configuration after mixing with HA.…”

Section: Resultsmentioning

confidence: 99%

Precision Therapy of Recurrent Breast Cancer through Targeting Different Malignant Tumor Cells with a HER2/CD44‐Targeted Hydrogel Nanobot

Chen

Sun

et al. 2023

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Heterogeneity and drug resistance of tumor cells are the leading causes of incurability and poor survival for patients with recurrent breast cancer. In order to accurately deliver the biological anticancer drugs to different subtypes of malignant tumor cells for omnidirectional targeted treatment of recurrent breast cancer, a distinct design is demonstrated by embedding liposome‐based nanocomplexes containing pro‐apoptotic peptide and survivin siRNA drugs (LPR) into Herceptin/hyaluronic acid cross‐linked nanohydrogels (Herceptin‐HA) to fabricate a HER2/CD44‐targeted hydrogel nanobot (named as ALPR). ALPR delivered cargoes to the cells overexpressing CD44 and HER2, followed by Herceptin‐HA biodegradation, subsequently, the exposed lipid component containing DOPE fused with the endosomal membrane and released peptide and siRNA into the cytoplasm. These experiments indicated that ALPR can specifically deliver Herceptin, peptide, and siRNA drugs to HER2‐positive SKBR‐3, triple‐negative MDA‐MB‐231, and HER2‐negative drug‐resistant MCF‐7 human breast cancer cells. ALPR completely inhibited the growth of heterogeneous breast tumors via multichannel synergistic effects: disrupting mitochondria, downregulating the survivin gene, and blocking HER2 receptors on the surface of HER2‐positive cells. The present design overcomes the chemical drug resistance and opens a feasible route for the combinative treatment of recurrent breast cancer, even other solid tumors, utilizing different kinds of biological drugs.

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Section: Resultsmentioning

confidence: 99%

Precision Therapy of Recurrent Breast Cancer through Targeting Different Malignant Tumor Cells with a HER2/CD44‐Targeted Hydrogel Nanobot

Chen

Sun

et al. 2023

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“…Lipophilic small molecules enter cells through passive diffusion, while the endocytoses of small molecules or macromolecules (which are similar to endogenous substances) principally depend on the transporters generated on the cell membrane. The nanoscale colloidal particles largely enter cells via endocytosis; additionally, a small number of these particles enter the cell by fusion with or destabilisation of the cytomembrane [24]. There are four major pathways for the endocytosis of extrinsic nanoparticles: clathrin-mediated endocytosis, caveolaemediated endocytosis, macropinocytosis, and the pathway independent of clathrin and caveolae [25][26][27].…”

Section: Resultsmentioning

confidence: 99%

DOC‐LS, a new liposome for dermal delivery, and its endocytosis by HaCaT and CCC‐ESF‐1 cells

Zhang

et al. 2018

IET nanobiotechnol.

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The main objective of this work was to investigate the uptake channels of skin cells through which coumarin 6, transported by deoxycholate-mediated liposomes (DOC-LS), was internalised; this was also compared against the action of conventional LS. Coumarin 6-loaded DOC-LS and LS were characterised for size distribution, zeta potential, and shape, and analysed in vitro in human epidermal immortal keratinocyte (HaCaT) (epidermal) and human embryonic skin fibroblast (CCC-ESF-1) (dermal) cell lines. Various endocytosis inhibitors were incubated with cells treated with the nanocarriers. Flow cytometry results indicated that HaCaT and CCC-ESF-1 cells internalise the tested preparations through pinocytotic vesicles, macropinocytosis, clathrin-mediated endocytic pathways, and via lysosomes, which consume a considerable amount of energy. The endocytosis pathways of DOC-LS and LS showed no difference. This study provides a basis for the application of LS being combined with a microneedle system for efficient intracellular drug delivery, targeting cutaneous histocyte disorders.

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Methods for Molecular Imaging, Detection and Visualization of CPPs

Langel

2023

CPP, Cell-Penetrating Peptides

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Effect of inserted spacer in hepatic cell-penetrating multifunctional peptide component on the DNA intracellular delivery of quaternary complexes based on modular design

Cited by 8 publications

References 23 publications

Precision Therapy of Recurrent Breast Cancer through Targeting Different Malignant Tumor Cells with a HER2/CD44‐Targeted Hydrogel Nanobot

Precision Therapy of Recurrent Breast Cancer through Targeting Different Malignant Tumor Cells with a HER2/CD44‐Targeted Hydrogel Nanobot

DOC‐LS, a new liposome for dermal delivery, and its endocytosis by HaCaT and CCC‐ESF‐1 cells

Methods for Molecular Imaging, Detection and Visualization of CPPs

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