The plasma triglyceride levels of rats fed diets containing sucrose were higher than those of rats fed starch. The presence of saturated fat in the diet further elevated these levels. No significant differences were found in the activities of hepatic lipogenic enzymes in groups fed saturated or polyunsaturated fat, although these activities were much higher after feeding sucrose. The activities of lipogenic enzymes in the adipose tissue were very low. It is suggested that sucrose elevates plasma triglycerides by stimulating hepatic lipogenesis, whereas saturated fat may produce the same effect by reducing plasma clearance. In cockerels, no significant difference was found in the plasma triglyceride levels of those fed either sucrose or starch or with saturated or polyunsaturated fat. Sucrose increased hepatic fatty acid synthetase activity, while polyunsaturated fat decreased it. It was presumed that efficient plasma clearance prevented any rise in plasma triglyceride concentrations.