Effect of insulin-like growth factor-1 complex of Simmental bull seminal plasma on post-thawed Kacang buck semen fertility

Abstract

Background and Aim: Kacang buck sperm is cryosensitive due to the seminal plasma of semen itself. Meanwhile, bull seminal plasma contains the insulin-like growth factor-1 (IGF-1) complex, which is cryoprotective. The addition of the crude protein of Simmental bull seminal plasma increased the quality of post-thawed semen of Kacang buck. The study was conducted to determine the effects of Simmental bull seminal plasma with IGF-1 on the fertility of post-thawed Kacang buck semen. Materials and Methods: Buck semen was diluted in the following skim milk-egg yolk extender preparations: Without the addition of Simmental bull seminal plasma IGF-1 complex protein (T0); with the addition of 12-μg Simmental bull seminal plasma IGF-1 complex protein (T1); and with the addition of 24-μg Simmental bull seminal plasma IGF-1 complex protein (T2). The extended semen was packed in 0.25-mL straws and frozen. Post-thawed semen fertility was evaluated based on the following variables: Sperm motility, viability, intact plasma membrane (IPM), malondialdehyde (MDA) levels, capacitation status, and acrosome reaction. The difference in each variable among the groups was evaluated using analysis of variance, followed by Tukey's honestly significant difference test, at a 95% level of significance. Meanwhile, principal component analysis (PCA) was used to identify the principal component of semen fertility among the seven parameters. Results: The T1 group showed the highest sperm motility, viability, IPM, and percentage of incapacitated sperm and the lowest MDA levels, percentage of capacitated sperm, and acrosome reaction. PCA revealed that sperm motility had a moderate to very robust correlation with other variables and is the most crucial parameter, accounting for 80.79% of all variables. Conclusion: The IGF-1 complex in Simmental bull seminal plasma was useful for increasing the fertility of post-thawed Kacang buck semen, and sperm motility was the principal component of semen fertility.

Effect of white guava (Psidium guajava L.) fruit juice on the quality of lead acetate induced rats (Rattus norvegicus) spermatozoa

“…Increased spermatozoa motility was caused by antioxidants, which defended the plasma membrane from lipid peroxidation caused by lead acetate so that the integrity of the spermatozoa plasma membrane remained intact. The plasma membrane is vital for spermatozoa motility (Susilowati et al 2021). Providing adequate antioxidants could also increase the body's metabolism and accelerate the formation of ATP which is used as an energy source to move flagella so that spermatozoa motility would increase (Park and Pang, 2021).…”

Contributions of seminal plasma proteins to fertilizing ability of bull sperm: A meta‐analytical review

“…These molecules modulate sperm capacitation (Karunakaran & Devanathan, 2015), protect sperm against lipid peroxidation (Patel et al, 2015), and stabilize the acrosome membrane (Alvarez‐Gallardo et al, 2013; Pande et al, 2018), maintaining the integrity of sperm structure. In addition, IGFs improve metabolic conditions and changes in fresh and thawed sperm (Costa et al, 2020; Singh et al, 2020; Susilowati et al, 2021). The IGF‐I binds to its receptors on the bovine spermatozoa membrane and maintains its motility through energy metabolism (Susilowati et al, 2021).…”

“…In addition, IGFs improve metabolic conditions and changes in fresh and thawed sperm (Costa et al, 2020; Singh et al, 2020; Susilowati et al, 2021). The IGF‐I binds to its receptors on the bovine spermatozoa membrane and maintains its motility through energy metabolism (Susilowati et al, 2021). IGF1 increases glucose uptake (Stewart & Rotwein, 1996), maintaining insulin levels, preventing the sperm from mobilizing their reserves, and maintaining membrane stability.…”

Calliandra honey and nutritional deficiency influence to spermatozoa motility and viability in Rattus norvegiccus

“…Spermatozoa observations were carried out in one field of view and counted at least 100 spermatozoa. Spermatozoa progressive motility calculation results are determined in percentage [18]. Observations were made on live and dead spermatozoa that identified by brigthly transparant head (alive) and purple/red colored head (dead) [19].…”