Effect of insulin on the glucose uptake of protozoa

Csaba, G.; Lantos, T.

doi:10.1007/bf02326980

Cited by 80 publications

(31 citation statements)

References 3 publications

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“…Ac cumulating evidence shows the effects of insu lin on processes in other microorganisms. In sulin added to N. crassa enhanced growth and stimulation of glucose oxidation in those fun gal cells [25], Insulin-increased glucose uptake by T. pyriformis was also reported [31]. In creased oxygen uptake by Acetabularia medi terránea was noted upon adding insulin to the medium [32].…”

Section: Discussionmentioning

confidence: 96%

Effects of Insulin and Glucose Tolerance Factor on Glucose Uptake by Yeast Cells

Mirsky

Berdicevsky

1994

Neurosignals

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Addition of mammalian insulin or glucose tolerance factor (GTF), to Saccharomyces cerevisiae ATCC 7752, increased 2-deoxyglucose uptake by yeast cells. The enhancement was dose-dependent, and also dependent on preincubation of the cells with glucose, prior to the addition of the hormone. The effect of insulin on glucose uptake by the yeast was very similar to the effect of GTF on these cells. Insulin and GTF increased also the growth of yeast cells, while denatured insulin or glucagon failed to act. Our findings support the view that both insulin and GTF might regulate carbohydrate metabolism in microorganisms.

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Section: Discussionmentioning

confidence: 96%

Effects of Insulin and Glucose Tolerance Factor on Glucose Uptake by Yeast Cells

Mirsky

Berdicevsky

1994

Neurosignals

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“…17 Exogeneously administered insulin promotes glucose uptake by the cell 18 and increases its cell division capacity. 19 This is similar to that seen in higher animals.…”

Section: Introductionmentioning

confidence: 99%

Prolonged elevation of insulin content in the unicellular tetrahymena after insulin treatment: induction of insulin production or storage?

Csaba

Gaál

Kovács

et al. 1999

Cell Biochem. Funct.

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In the unicellular organism, Tetrahymena, the ®rst encounter with an exogeneously given hormone results in hormonal imprinting. This causes an increase of the binding capacity of receptors and the production of the appropriate hormone in the progeny generations of the treated cell. In the present experiments the quantity (using radioimmunoassay) and localization (using confocal laser scanning microscopy) of the immunologically insulin-like material (hereafter insulin) were studied for 10 days after 4 h or 24 h 10 À6 M insulin treatment (hormonal imprinting). Forty-eight hours after both insulin treatments a high quantity of insulin was present in the cells. This value was also signi®cantly increased after 96 h. After 8 days the dierence to the control was signi®cant only in the 24 h treated group. Confocal microscopy (using antibody to pig insulin) localized insulin in the cell body. The oral ®eld contained extremely high quantities of the endogeneous hormone. Insulin treatment (after 48 and 96 h) caused an elevation of insulin content in general, and speci®c accumulation in the posterior sections of the cell, around the nucleus and in the periphery were observed. Ten days after both treatments only the peripheral region of the cell body and the ciliary row contained more insulin than the control. This means that after insulin treatment the quantity of insulin increases for a lengthy time period which is followed by the expression of insulin in the peripheral region. Insulin contained by Tetrahymena 48 h after imprinting stimulated glucose uptake of rat diaphragm.

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“…[1][2][3] Later LeRoith et al [4][5][6][7] and others 8,9 studied the presence of vertebrate hormones also in Tetrahymena and these were found, and the signal transduction pathways that were similar to the mammalian ones were also demonstrated. 10,11 The structure of the insulin receptor of Tetrahymena was also investigated and found similar to the mammalian receptor.…”

Section: Introductionmentioning

confidence: 94%

Effect of concanavalin A (Con‐A) on the hormone production of the unicellular Tetrahymena and the immune cells of the rat. A comparative study

Csaba

Pállinger

2008

Cell Biochemistry & Function

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Tetrahymena populations were treated with 10(-15) g ml(-1) or 10(-6) g ml(-1) concanavalin-A (Con-A) in tryptone-yeast medium for 1 h. Rat peritoneal immune cells (mast cells, lymphocytes, monocyte-granulocyte group) were also treated with 10(-6) g ml(-1) Con-A, for 1 h. The cells' hormone (ACTH, histamine, serotonin, endorphin, triiodothyronine (T(3))) content was measured by using immunocytochemistry and flow cytometry. The extremely low dose of Con-A universally and significantly elevated the hormone contents, while the result of higher dose was uncertain. In the immune cells, Con-A significantly decreased the ACTH level in each cell type and histamine level in mast cells. The results demonstrate the very high sensitivity of Tetrahymena receptors for a non-hormone (lectin) molecule, which can bind to the insulin receptors and mimics the effect of insulin. The results also show that Tetrahymena receptors are more sensitive to lower concentrations of molecules than to higher ones. The universal hormone-production stimulating effect of Con-A-which is observed in Tetrahymena-is specified in rat.

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Effect of insulin on the glucose uptake of protozoa

Abstract: Insulin stimulates the glucose uptake of Tetrahymena pyriformis. This shows the presence of insulin receptors in Tetrahymena, consequently receptors may be present in a level of phylogenesis, where the natural contact between the given hormone and the cell is unnecessary and impossible.

Cited by 80 publications

References 3 publications

Effects of Insulin and Glucose Tolerance Factor on Glucose Uptake by Yeast Cells

Effects of Insulin and Glucose Tolerance Factor on Glucose Uptake by Yeast Cells

Prolonged elevation of insulin content in the unicellular tetrahymena after insulin treatment: induction of insulin production or storage?

Effect of concanavalin A (Con‐A) on the hormone production of the unicellular Tetrahymena and the immune cells of the rat. A comparative study

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