Effect of Interleukin-1 Receptor Antagonist on Concanavalin A Response of Peripheral Blood Mononuclear Cells from Newborn Calves.

Yamanaka, Hitoki; Katagiri, Yoshito; Domi, Mayumi; Hagiwara, Kaoru; Kirisawa, Rikio; Iwai, Hiroshi

doi:10.1292/jvms.63.933

Cited by 4 publications

(11 citation statements)

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“…However, mitogenic activities of PBMC from colostrum-fed calves were significantly higher than those of PBMC from colostrum-deprived calves after stimulation with ConA [7]. Indeed, we confirmed that coexistence of IL-1ra with IL-1β in colostrum had no effect on the mitogenic response of neonatal PBMC [21]. The cytokine inhibitors in colostrum may play a role in amelioration of the adverse effects of cytokines as suggested by Buescher and Malinowska [1].…”

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confidence: 81%

“…Sera were stored at -30°C until use. PBMC from newborn calves (n=10) were prepared from heparinized peripheral blood of healthy calves at birth to 28 days after birth as described previously [21].The concentrations of five cytokines (IL-1β, IL-6, TNF-α, IFN-γ and IL-1ra) in sera were determined by sandwich ELISA using affinity-purified IgGs specific for each cytokine as described previously [5,6,20]. These affinity-purified IgGs from rabbit immunized with each recombinant bovine cytokine were used for capture ELISA.…”

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confidence: 99%

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Transient Detection of Proinflammatory Cytokines in Sera of Colostrum-Fed Newborn Calves

Yamanaka

Hagiwara

Kirisawa

et al. 2003

The Journal of Veterinary Medical Science

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ABSTRACT. To obtain basic information on the state of proinflammatory cytokines in newborn calves, we determined the kinetics of 5 cytokines (IL-1β, IL-6, TNF-α, IFN-γ and IL-1 receptor antagonist) in sera of newborns during the first 4 weeks of life. At birth, none of the 5 cytokines were detected in almost all serum samples, but the cytokines became detectable within 12 hr after being fed colostram. The mean concentrations of the cytokines reached peak levels by 24 hr and then gradually decreased and became undetectable by 4 weeks after birth. Cytokine mRNA expressions in peripheral blood mononuclear cells of newborns were observed without reference to the cytokine concentrations in sera. Serum cytokines detected in newborn calves are probably colostral origin. KEY WORDS: calf-serum, colostrum, cytokine.J. Vet. Med. Sci. 65 (7): [813][814][815][816] 2003 Resistance to infection is determined by the host immune state. The immune state of neonatal calves is largely dependent on the colostrum they are fed. Immunoglobulin, one of resistant factors present in colostrum, is known to play a protective role. The presence of several cytokines in bovine colostrum has been shown by several investigators [4][5][6]15]. In addition, oral administration of recombinant bovine (rb) IL-1β, one of the colostral cytokines, to neonatal calves resulted in the appearance of the cytokine in circulation and the activation of circulating lymphocytes and neutrophils [7]. Furthermore, and TNF-α and IFN-γ [17], which are also present in bovine colostrum [6], have been reported to potentiate immunological functions. Therefore, cytokines transferred to neonates via colostrum are considered to contribute to maturation of neonatal immune functions.To obtain basic information on the state of cytokines in neonatal calves, we measured the levels of five cytokines (IL-1β, IL-6, TNF-α, IFN-γ and IL-1 receptor antagonist, IL-1ra) in serum samples and expression of these cytokine mRNAs in peripheral blood mononuclear cells (PBMC) obtained from neonatal calves after they had been fed colostrum.Specimens were collected from Holstein Friesian cattle that were kept at the dairy farm of Rakuno Gakuen University. Blood was obtained by cervical vessel puncture using a sterile vacuum syringe from healthy calves at birth before being fed colostrum (n=21) and at 12 hr (hr) (n=19), 1 day (n=21), 2 days (n=18), 3 days (n=18), 5 days (n=17), 7 days (n=16), 14 days (n=13), 21 days (n=11) and 28 days (n=11) after birth. Newborn calves were fed about 2 l of colostrum from respective dams (n=10) or pools (n=11) within 2 hr after birth and then fed colostrum from respective dams two times a day by 5 days after birth. The other 4 calves, as controls, fed formula as substitute for colostrum for 3 days and their blood were collected during this period. Sera were stored at -30°C until use. PBMC from newborn calves (n=10) were prepared from heparinized peripheral blood of healthy calves at birth to 28 days after birth as described previously [21].The concentrations of...

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Transient Detection of Proinflammatory Cytokines in Sera of Colostrum-Fed Newborn Calves

Yamanaka

Hagiwara

Kirisawa

et al. 2003

The Journal of Veterinary Medical Science

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“…Newborn calves were fed about 2 liters of colostrum within 2 hr after birth. PBMC were obtained from heparinized peripheral blood as described previously (34).…”

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“…The blastogenic activity of the cells in culture was evaluated by an MTT assay (20). The procedure used for the culture has been described previously (34). Briefly, 1ϫ10 5 of PBMC in RPMI-1640 medium (Sigma, U.S.A.) containing 10% FCS was added to each well of a 96-well microculture plate (Falcon, U.S.A.) along with serially diluted whey or recombinant cytokines.…”

Section: Methodsmentioning

confidence: 99%

Proinflammatory Cytokines in Bovine Colostrum Potentiate the Mitogenic Response of Peripheral Blood Mononuclear Cells from Newborn Calves through IL‐2 and CD25 Expression

Yamanaka

Hagiwara

Kirisawa

et al. 2003

Microbiology and Immunology

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Colostrum contains various agents to compensate for insufficiency of nutrient and immune components in newborns, such as immunoglobulins, lactoferrin, and vitamins (25). In addition, colostrum feeding is considered to enhance T cell-mediated responses and to improve B cell immunity in the immature immune systems in newborns (21,22). Our previous study showed the presence of high concentrations of IL-1β, IL-6, TNF-α, IFN-γ and IL-1 receptor antagonist (ra) in bovine colostrum (13). It is likely that colostral cytokines are transferred to newborn calves (12,14,33). These cytokines, except for IL-1ra, potentiate immunological functions (4,8,23,29,31). Therefore, colostral cytokines are considered to play important roles in development of immunologic functions of neonates (2, 27).Indeed, our previous study demonstrated transfer of orally administered recombinant bovine (rb) IL-1β into serum of neonatal calves and activation of circulating lymphocytes and neutrophils by this cytokine (14).In the present study, to determine the effects of colostral cytokines on neonatal immune functions, we investigated the effect of each of the cytokines mentioned above on the blastogenic activity toward concanavalin A (ConA) of PBMC obtained from newborn calves before the calves had been fed colostrum. In addition, we also examined the expression of IL-2 mRNA and the expression of IL-2 receptor α chain, i.e., CD25, in these PBMC, since IL-2 has been shown to be crucial for the clonal expansion of T cells and growth of B cells and since α chains are expressed on activated cells Proinflammatory Cytokines in Bovine Colostrum

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Citocinas IL-1β, IL-6, TNF-α e IFN-γ no sangue e colostro de fêmeas bovinas da raça Holandesa. Importância na transferência de imunidade passiva

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Agradecimentos À Deus, que me abençoa e me fortalece todos os dias; Ao meu orientador, Prof. Dr. Fernando José Benesi, por toda a ajuda e conselhos não somente relacionados á este trabalho, mas sobre a vida também. À amiga e Profa. Dra. Viviani Gomes, fundamental na minha vida. Agradeço todos os dias por tê-la em meu caminho! Você é muito importante para mim. À Profa. Dra. Alice Maria Melville Paiva Della Libera, minha professora da graduação e que me ensinou os primeiros passos..... exemplos de pessoa e de profissional a serem seguidos e admirados. Ao Prof. Dr. Maurício Garcia, meu professor da graduação e responsável por despertar em mim o interesse pela pesquisa. À Dra. Gerlândia Neres Pontes, por todos os ensinamentos e acima de tudo por toda paciência e atenção. Ao veterinário Sérgio Soriano e toda a equipe da Fazenda Colorado, em especial a equipe da maternidade e sala de ordenha, que não mediram esforços para que eu pudesse finalizar esta pesquisa. pela eficiente ajuda no preparo e limpeza dos materiais utilizados na colheita. Aos queridos ex-alunos Evaldo Aparecido Dias da Rosa, Gisele Fernanda Greghi, Públio Domingues, Tarcísio Macedo Silva e Tatiana Ranieri, que perderam noites de sono e enfrentaram o frio na Fazenda para me ajudar nas colheitas. Sinto muitas saudades! Ao doutorando Rogério Batista, aos acadêmicos Vinícius Dayoub Gonçalves e Raquel Marques e às veterinárias Carolina Harumi Seino e Juliana Bombardelli por toda a ajuda com o preparo dos materiais e durante o processamento das amostras, e especialmente, à mestranda Carolina Lara Shecaira, pela ajuda madrugadas afora no laboratório e pela companhia nas viagens para as colheitas do colostro. À equipe "BVD", Camila Ramos e Cynthia Pereira da Costa e Silva, por todo o apoio e alto astral que demonstraram, até nos momentos mais difíceis.

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Effect of Interleukin-1 Receptor Antagonist on Concanavalin A Response of Peripheral Blood Mononuclear Cells from Newborn Calves.

Cited by 4 publications

References 20 publications

Transient Detection of Proinflammatory Cytokines in Sera of Colostrum-Fed Newborn Calves

Transient Detection of Proinflammatory Cytokines in Sera of Colostrum-Fed Newborn Calves

Proinflammatory Cytokines in Bovine Colostrum Potentiate the Mitogenic Response of Peripheral Blood Mononuclear Cells from Newborn Calves through IL‐2 and CD25 Expression

Citocinas IL-1β, IL-6, TNF-α e IFN-γ no sangue e colostro de fêmeas bovinas da raça Holandesa. Importância na transferência de imunidade passiva

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