Effect of interleukin‐12 gene expression on insulin resistance in patients with acne vulgaris

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BackgroundAcne vulgaris (AV) is a chronic inflammatory skin condition affecting the pilosebaceous unit, commonly presenting as comedones, papules, pustules, or nodules on the face, upper limbs, torso, and back, with comedones formation being the primary pathology leading to disfiguring inflammation, hyperpigmentation, scarring, and psychological impact.AimThe purpose of this study was to investigate the significance of two genetic variants in the promoter region of the tumor necrosis factor‐alpha (TNF‐α) gene and their association with insulin resistance (IR) in acne patients. To understand how these variants contribute to AV and its associated IR.Subjects and methodsAn analytical cross‐sectional study with a case‐control design and research evaluation was carried out on 87 AV patients and 73 healthy volunteers. The medical histories of both groups were obtained, as well as the severity and duration of inflammation among acne sufferers, as well as demographic data. Biochemical analysis was performed on both sets of participants, including fasting blood glucose levels, insulin levels while fasting, IR, and serum TNF‐α. PCR‐RFLP analysis identified −863 G > A (rs1800630) and −308 G > A (rs1800629) variations, and real‐time PCR analysis evaluated TNF‐α gene expression in both patients and healthy people.ResultsAcne patients exhibited significantly higher levels of IR, fasting glucose, fasting insulin, serum TNF‐α, and TNF‐α folding change, when compared to healthy controls. The co‐dominant model for −863 G > A and −308 G > A variants exhibited significant variations between the two groups. Severe acne patients who had the A/A genotype for −308 variants exhibited higher levels of IR, serum TNF‐α, and TNF‐α folding change. Highly significant positive linear correlation between IR, serum TNF‐α, and TNF‐α folding change in severe AV.ConclusionThere is a correlation between AV, especially severe acne, and the −863 G > A and −308 G > A polymorphism, which influences TNF‐α gene expression and serum TNF‐α levels.

Section: Discussionmentioning

confidence: 99%

In severe acne vulgaris, TNF‐α gene variants are connected to increased TNF‐α gene expression and insulin resistance

AbdElneam,

Alhajlah,

Al‐Dhubaibi

et al. 2024

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“…14 In our population, several studies have been conducted to understand the relationship between genetic factors and dermatological conditions. [15][16][17][18][19][20][21][22][23][24] However, according to our knowledge, no study tries to determine the mechanisms by which miRNAs contribute to AA.…”

Section: Introductionmentioning

confidence: 99%

MiR‐200c‐3p as a novel genetic marker and therapeutic tool for alopecia areata

AbdElneam,

Al‐Dhubaibi,

Bahaj

et al. 2024

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BackgroundMicroRNAs (miRNAs) are small RNA molecules that regulate gene expression in diverse biological processes. They hold promise as therapeutic candidates for targeting human disease pathways, although our understanding of their gene regulatory mechanism remains incomplete. Alopecia areata (AA) is a prevalent inflammatory ailment distinguished by the infiltration of T cells targeting the anagen‐stage hair follicles. The scarcity of effective remedies for AA may stem from limited understanding regarding its precise cellular mechanism.AimTo investigate and examine the importance and role of the miR‐200c‐3p as a genetic indicator for AA, and its possible impact on disease progression.Subjects and methodsCase‐control study included 65 patients with AA and 65 matched healthy controls. A real‐time PCR technique was used to measure the expression of miR‐200c‐3p for both groups. Bioinformatic tools were used for prediction with genes and gene‐gene interaction, and protein‐protein interaction.ResultsThe expression levels of miR‐200c‐3p were significantly higher in AA patients than in healthy controls. We predicted that miR‐200c‐3p plays a markable role in the development of AA by its effect on the EGFR tyrosine kinase inhibitor resistance pathway.ConclusionWe were able to identify the influence of miR‐200c‐3p on both PLCG1 and RPS6KP1 genes which in turn regulate the EGFR tyrosine kinases resistance pathway that displayed the most substantial increase in activity. Our outcomes shed light on the era of the potential theranostic role of this innovative miRNA in AA.

“…Autoimmune processes in AA may be impacted by vitamin D regulation of immune responses through VDR. 5 Several studies in our population have tried to find the relation between different biochemical parameters and different skin diseases such as acne, [6][7][8][9][10] and psoriasis vulgaris [11][12][13][14][15] but according to our knowledge, there is no study able to find the relation between CRP and vitamin D in AA patients.…”

Section: Introductionmentioning

confidence: 99%

C‐reactive protein as a novel biomarker for vitamin D deficiency in alopecia areata

AbdElneam,

Al‐Dhubaibi,

Bahaj

et al. 2024

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BackgroundAlopecia areata (AA) is an autoimmune condition characterized by sudden and unpredictable hair loss, with a lifetime incidence of 2%. AA can be divided into three categories: patchy alopecia, alopecia totalis, and alopecia universalis. It can affect a person's psychological health and overall quality of life. Elevated C‐reactive protein (CRP) levels in the liver may indicate an inflammatory response in autoimmune diseases. Vitamin D, essential for immune system control and skin health, may be related to AA. Hair follicles contain vitamin D receptors, which control immunological responses in the skin. However, no study has found a relationship between CRP and vitamin D in AA patients in our region.Subjects and MethodsAn analytical cross‐sectional study with a case‐control design research investigation of 82 AA patients and 81 healthy controls was carried out. Both groups’ medical histories were taken. Biochemical analysis was done for both groups as well as the serum vitamin D levels, and CRP. Genetic analysis for CDX2 rs11568820 variant detected by PCR (T‐ARMS‐PCR) method and vitamin D receptor (VDR) gene expression measured by real‐time PCR analysis for both patients and healthy subjects.ResultsCRP levels are higher in AA patients, AA patients with G/G genotypes exhibited higher concentrations of CRP when compared to those with A/A and A/G genotypes while patients with A/A genotypes have higher levels of Serum vitamin D as compared to the A/G and G/G genotypes. G allele was more abundant in AA patients. VDR gene expression was lower in AA compared to control and lower in ophiasis compared to localized and multiple patchy AA. An important inverse linear correlation was observed between vitamin D and CRP levels in ophiasis AA.ConclusionCRP concentrations were found to be elevated in AA patients. The considerable accuracy of CRP in the diagnosis of AA is substantiated by a statistically significant al. A noteworthy inverse linear association was observed between serum vitamin D and CRP concentrations in ophiasis AA.