Effect of interleukin-4 on interleukin-2-dependent generation of natural killer cells

TNFα processing enzyme inhibitors prevent aspirin‐induced TNFα release and protect against gastric mucosal injury in rats

“…Total RNA was alcohol‐precipitated and the pellet dissolved in 100 m M Tris, 1 m M EDTA buffer. The RNA, which ends in a 3′‐poly(A + ) tail released from cells during the lysis step, was enriched by a batch of oligo(dT)‐as primer and examined by Northern blot analysis as previously reported, 24 using 1% agarose–formaldehyde gel. The separated RNA was transblotted to nitrocellulose, baked for 3 h at 80 °C, and hybridized overnight at 42 °C with a hybridizing solution containing 50% v/v formamide in 5 × standard saline citrate buffer, 1% v/v sodium dodecyl sulphate, and [ 32 P]5′‐end‐labelled complementary DNA (cDNA).…”

Involvement of lnterleukin-4 and Soluble CD23 in Hypersynthesis of Immunoglobulins A and E in Patients with IgA Nephropathy

“…Interleukin-4 (IL-4) has shown pleiotropic effects on preactivated B cells [1], T cells [2], monocytes [3], thymo cytes [4] and natural killer cell clones [5], With B lympho cytes and monocytes, 11-4 has been found to be a major inducer of the 45-kD form of the CD23 molecule on the cell surface [1,6]. This molecule is a low-affinity receptor for IgE and is cleaved by proteolysis resulting in the stable 25-kD form of soluble CD23 (sCD23) [7], Unlike the oth- er Fc receptors, it does not belong to the immunoglobulin gene superfamily but is a member of a primitive family of Ca2+-dependent lectins [8].…”