Effect of intermittent teriparatide (PTH 1-34) on the alveolar healing process in orchiectomized rats

Oliveira, Danila de; Puttini, Igor de Oliveira; Gomes-Ferreira, Pedro Henrique Silva; Palin, Letícia Pitol; Matsumoto, Mariza Akemi; Okamoto, Roberta

doi:10.1007/s00784-018-2672-y

Cited by 15 publications

(10 citation statements)

References 37 publications

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“…In the final period (42 days), in non-critical defects in rats, the process would progress to complete repair of the surgical area. In the case of critical defects, which do not repair spontaneously until the end of the experiment, the evaluation of the formation of new bone is important to analyze the action of photobiomodulation and grafting materials in the evolution of the process, in addition to the amount of tissue non-mineralized material that remained inside the defect and the permanence of biomaterials at this site [ 48 , 49 , 50 , 51 , 52 ].…”

Section: Resultsmentioning

confidence: 99%

Effects of a Biocomplex Formed by Two Scaffold Biomaterials, Hydroxyapatite/Tricalcium Phosphate Ceramic and Fibrin Biopolymer, with Photobiomodulation, on Bone Repair

et al. 2022

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There are several treatment methods available for bone repair, although the effectiveness becomes limited in cases of large defects. The objective of this pre-clinical protocol was to evaluate the grafting of hydroxyapatite/tricalcium phosphate (BCP) ceramic biomaterial (B; QualyBone BCP®, QualyLive, Amadora, Portugal) together with the heterologous fibrin biopolymer (FB; CEVAP/UNESP Botucatu, Brazil) and with photobiomodulation (PBM; Laserpulse®, Ibramed, Amparo, Brazil) in the repair process of bone defects. Fifty-six rats were randomly divided into four groups of seven animals each: the biomaterial group (G1/B), the biomaterial plus FB group (G2/BFB); the biomaterial plus PBM group (G3/B + PBM), and the biomaterial plus FB plus PBM group (G4/BFB + PBM). After anesthesia, a critical defect was performed in the center of the rats’ parietal bones, then filled and treated according to their respective groups. The rats were euthanized at 14 and 42 postoperative days. Histomorphologically, at 42 days, the G4/BFB + PBM group showed a more advanced maturation transition, with more organized and mature bone areas forming concentric lamellae. A birefringence analysis of collagen fibers also showed a more advanced degree of maturation for the G4/BFB + PBM group. In the comparison between the groups, in the two experimental periods (14 and 42 days), in relation to the percentage of formation of new bone tissue, a significant difference was found between all groups (G1/B (5.42 ± 1.12; 21.49 ± 4.74), G2/BFB (5.00 ± 0.94; 21.77 ± 2.83), G3/B + PBM (12.65 ± 1.78; 29.29 ± 2.93), and G4/BFB + PBM (12.65 ± 2.32; 31.38 ± 2.89)). It was concluded that the use of PBM with low-level laser therapy (LLLT) positively interfered in the repair process of bone defects previously filled with the biocomplex formed by the heterologous fibrin biopolymer associated with the synthetic ceramic of hydroxyapatite and tricalcium phosphate.

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Section: Resultsmentioning

confidence: 99%

Effects of a Biocomplex Formed by Two Scaffold Biomaterials, Hydroxyapatite/Tricalcium Phosphate Ceramic and Fibrin Biopolymer, with Photobiomodulation, on Bone Repair

et al. 2022

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“…De Oliveira et al (2019) [ 59 ] observed that the teriparatide intermitent treatment in orchiectomized rats promoted an increase of bone volume percentage, as well as the improvement of trabeculae thickness, evidencing the positive effect on the formation of healthy bone. When evaluating whether the reparative alveolus filled with Biogran ® functionalized or not with PTH, 1-34 it was seen that there was also quality bone formation.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, TRAP showed mild labeling for the treated groups, showing a local anabolic effect of teriparatide in opposition to osteoclast resorption activity. Studies of de Oliveira et al and Oliveira Puttini et al showed that the use of systemic teriparatide after exodontia in orchiectomized rats led to increased expression for Wnt and osteocalcin proteins [ 59 ], a balance between bone formation and resorption for OPG and RANKL proteins [ 29 ] and lower labeling for TRAP protein [ 59 ], indicating increased repair tissue formation and less alveolar bone resorption. Therefore, from the results presented, it can be inferred that the use of Biogran ® promoted slight improvement in rat alveolar repair and that, with the addition of PTH 1-34 topic, this condition tends to improve, resembling bone repair by clot formation.…”

Section: Discussionmentioning

confidence: 99%

Effect of Topical PTH 1-34 Functionalized to Biogran® in the Process of Alveolar Repair in Rats Submitted to Orchiectomy

et al. 2021

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(1) Background: There are many therapies for osteoporosis control and bone maintenance; anabolic drugs such as teriparatide and bone grafts help in the repair process and stimulate bone formation. Thus, the aim of the present study was to evaluate the behavior of repaired bone in the presence of PTH (teriparatide) associated with Biogran® (biomaterial) through a sonochemical procedure after extraction in rats. (2) Methods: The insertion of Biogran® with PTH in the alveolus was performed 30 days after incisor extraction. Euthanasia occurred after 60 days. (3) Results: The use of local treatment of PTH loaded with Biogran® in healthy rats promoted good results for micro-CT, with an increase in percentage and bone volume, number and trabecular separation and less total porosity. Greater immunostaining for Wnt, β-Catenin and osteocalcin proteins and lower expression for Thrombospondin-Related Adhesive Protein (TRAP), which shows an increase in the number of osteoblasts and inhibition of osteoclast action. However, the treated orchiectomized groups did not obtain such expressive results. (4) Conclusion: The use of Biogran® with PTH improved alveolar repair in rats. However, new researches with more efficient doses must be studied to collaborate effectively with the formation of a quality bone after the orchiectomy.

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“…iPTH therapy through subcutaneous or intra-oral injection were both effective in promoting healing in the tooth extraction socket by increasing new bone formation and connective tissue maturation ( Kuroshima et al, 2013 ). In teriparatide treated osteoporosis rats, the extraction socket exhibited increased expression levels of osteogenic markers including Wnt, Alp, and Ocn and reduced osteoclast numbers ( de Oliveira et al, 2019 ). In comparison with bisphosphonates, PTH has a better therapeutic effect in both hard and soft tissue regeneration at the extraction site by enhancing osteogenesis and collagen deposition while suppressing inflammation ( Kuroshima et al, 2014b ).…”

Section: Therapeutic Application Of Parathyroid Hormone/parathyroid Hormone-related Protein In Orofacial Regionmentioning

confidence: 99%

Parathyroid Hormone 1 Receptor Signaling in Dental Mesenchymal Stem Cells: Basic and Clinical Implications

Lyu

et al. 2021

Front. Cell Dev. Biol.

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Parathyroid hormone (PTH) and parathyroid hormone-related protein (PTHrP) are two peptides that regulate mineral ion homeostasis, skeletal development, and bone turnover by activating parathyroid hormone 1 receptor (PTH1R). PTH1R signaling is of profound clinical interest for its potential to stimulate bone formation and regeneration. Recent pre-clinical animal studies and clinical trials have investigated the effects of PTH and PTHrP analogs in the orofacial region. Dental mesenchymal stem cells (MSCs) are targets of PTH1R signaling and have long been known as major factors in tissue repair and regeneration. Previous studies have begun to reveal important roles for PTH1R signaling in modulating the proliferation and differentiation of MSCs in the orofacial region. A better understanding of the molecular networks and underlying mechanisms for modulating MSCs in dental diseases will pave the way for the therapeutic applications of PTH and PTHrP in the future. Here we review recent studies involving dental MSCs, focusing on relationships with PTH1R. We also summarize recent basic and clinical observations of PTH and PTHrP treatment to help understand their use in MSCs-based dental and bone regeneration.

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Effect of intermittent teriparatide (PTH 1-34) on the alveolar healing process in orchiectomized rats

Cited by 15 publications

References 37 publications

Effects of a Biocomplex Formed by Two Scaffold Biomaterials, Hydroxyapatite/Tricalcium Phosphate Ceramic and Fibrin Biopolymer, with Photobiomodulation, on Bone Repair

Effects of a Biocomplex Formed by Two Scaffold Biomaterials, Hydroxyapatite/Tricalcium Phosphate Ceramic and Fibrin Biopolymer, with Photobiomodulation, on Bone Repair

Effect of Topical PTH 1-34 Functionalized to Biogran® in the Process of Alveolar Repair in Rats Submitted to Orchiectomy

Parathyroid Hormone 1 Receptor Signaling in Dental Mesenchymal Stem Cells: Basic and Clinical Implications

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