Effect of interval between inseminations when using frozen-thawed boar sperm on fertility and fetal paternity in mature gilts

Ringwelski, Jennifer; Beever, Jonathon E.; Knox, Robert V.

doi:10.1016/j.anireprosci.2013.01.007

Cited by 20 publications

(28 citation statements)

References 44 publications

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“…Semen was diluted using Androstar Plus (MOFA, Verona, WI, USA) at 37°C, cooled to 17°C over 2 h and then shipped to the USDA in Fort Collins, Colorado for processing within 24 h of collection. Spermatozoa were separated from seminal plasma and further processed for freezing (Almlid & Johnson, 1988) in 0.5 mL straws at a final concentration of 1.4 9 10 9 spermatozoa/mL in egg yolk and glycerol (Ringwelski et al, 2013). Straws were then shipped in liquid nitrogen vapor to the University of Illinois.…”

Section: Sperm Collection and Freezingmentioning

confidence: 99%

“…Prior to AI, DNA was obtained from boar semen and gilt blood. A liver sample was obtained from each fetus for DNA genotyping to determine paternity as described by (Ringwelski et al, 2013). A panel of 14 microsatellite markers was chosen for PCR and products were combined, purified, and sequenced as described by (Meyers et al, 2010).…”

Section: Reproductive Tract Processing and Fetal Paternitymentioning

confidence: 99%

“…However, <1% of porcine AI use frozen-thawed boar spermatozoa because the economic advantages do not compensate for the reduction in fertility caused by cryopreservation damage (Johnson et al, 2000;Knox et al, 2008;Rath et al, 2009). Identifying cryopreserved ejaculates of acceptable fertility prior to use would be beneficial given the interest for enhancing the commercial use of frozen-thawed semen by the swine industry (Didion et al, 2013;McNamara & Knox, 2013;Ringwelski et al, 2013;Tom as et al, 2013;Estrada et al, 2014). Conventional laboratory evaluation of frozen-thawed boar spermatozoa has not historically provided accurate estimates of fertility (Gil et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Enhanced fertility prediction of cryopreserved boar spermatozoa using novel sperm function assessment

Daigneault

McNamara

Purdy³

et al. 2015

Andrology

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SUMMARYDue to reduced fertility, cryopreserved semen is seldom used for commercial porcine artificial insemination (AI). Predicting the fertility of individual frozen ejaculates for selection of higher quality semen prior to AI would increase overall success. Our objective was to test novel and traditional laboratory analyses to identify characteristics of cryopreserved spermatozoa that are related to boar fertility. Traditional post-thaw analyses of motility, viability, and acrosome integrity were performed on each ejaculate. In vitro fertilization, cleavage, and blastocyst development were also determined. Finally, spermatozoa-oviduct binding and competitive zonabinding assays were applied to assess sperm adhesion to these two matrices. Fertility of the same ejaculates subjected to laboratory assays was determined for each boar by multi-sire AI and defined as (i) the mean percentage of the litter sired and (ii) the mean number of piglets sired in each litter. Means of each laboratory evaluation were calculated for each boar and those values were applied to multiple linear regression analyses to determine which sperm traits could collectively estimate fertility in the simplest model. The regression model to predict the percent of litter sired by each boar was highly effective (p < 0.001, r 2 = 0.87) and included five traits;acrosome-compromised spermatozoa, percent live spermatozoa (0 and 60 min post-thaw), percent total motility, and the number of zona-bound spermatozoa. A second model to predict the number of piglets sired by boar was also effective (p < 0.05, r 2 = 0.57).These models indicate that the fertility of cryopreserved boar spermatozoa can be predicted effectively by including traditional and novel laboratory assays that consider functions of spermatozoa.

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Section: Sperm Collection and Freezingmentioning

confidence: 99%

Section: Reproductive Tract Processing and Fetal Paternitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Enhanced fertility prediction of cryopreserved boar spermatozoa using novel sperm function assessment

Daigneault

McNamara

Purdy³

et al. 2015

Andrology

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“…To evaluate plasma membrane integrity after sperm washing procedures, a subpopulation of spermatozoa was incubated with propidium iodide as described previously (Ringwelski et al, 2013). Few spermatozoa were stained with PI ( Figure S1) demonstrating that plasma membrane of >90% of the spermatozoa was intact throughout the experiments.…”

Section: Fluorescent Glycan-binding Assaymentioning

confidence: 99%

Multiple proteins from ejaculated and epididymal porcine spermatozoa bind glycan motifs found in the oviduct

et al. 2014

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SUMMARYPreviously, we demonstrated that porcine spermatozoa bind selectively to Lewis x (Le X ) and bi-antennary a2-6 sialylated N-acetyllactosamine (bi-SiaLN) glycan motifs found in the oviduct. Recognition of these motifs mediates sperm binding to oviduct epithelial cells to form a reservoir. Here, we characterize proteins that bind to Le X and bi-SiaLN in ejaculated and cauda epididymal spermato-) and bi-SiaLN were used to locate the binding proteins on spermatozoa. Binding to N-acetyllactosamine (LN), a glycan that spermatozoa bind less, was assessed as a control. Over 60% of ejaculated and epididymal spermatozoa bound to suLe X and bi-SiaLN, but only 25% bound to LN. Specific binding to suLe X and bi-SiaLN required divalent cations. Fluoresceinated suLe X bound mostly to the plasma membrane at the apex of the head, whereas bi-SiaLN bound to a broader area of the head. Further characterization of receptors was accomplished by probing, with specific glycans, sperm proteins that had been separated by SDS-PAGE and transferred to blots. Glycan blot assays identified several suLe X -binding bands that migrated as 12-250 kDa. Migration of some of the bands that bound bi-SiaLN was similar to those that bound suLe X . A 12-kDa band protein was detected in seminal fluid and ejaculated spermatozoa, but absent in epididymal spermatozoa. In summary, we determined that: (i) multiple glycan-binding proteins recognize suLe X and bi-SiaLN motifs, (ii) specific binding required divalent cations and (iii) glycanbinding proteins were identified before and after sperm contact with accessory gland fluids. While the identity of these molecules is still unknown, this information predicts characteristics of the authentic oviduct suLe X and bi-SiaLN receptors on spermatozoa.

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“…The differences in number of piglets born live between boars of the two breed types in this study were not significant (Table 1), instead, it is the value for Pietrain boars that stood out over the other categories. The establishment of AI centers for management of boars and production of semen has allowed for selection of boars for fertility and sperm production using in vitro and in vivo measures (Ringwelski et al, 2013;Knox, 2016).…”

Section: Discussionmentioning

confidence: 99%

Success drivers of pig artificial insemination based on imported fresh semen

Vincent¹,

Rugira²,

Dâ€TMAndre³

2018

Int. J. Livest. Prod.

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This study was conducted to assess the factors that affect pig litter size, proportion of live pigs at birth, number of inseminations per conception, and efficiency of artificial insemination. The main factors assessed were sow breed (n = 2), sire breed (n = 3), sow parity (n = 7) and insemination method (n = 2). The sow breeds used were Landrace, LL (n = 27) and Landrace × Pietran crossbreds, LP (n = 37); boar breeds used were Landrace (LL), Pietran (PP) and Landrace × Pietran crossbreds (LP). Sows were randomly inseminated either by boar or artificially, with semen freshly imported from Belgium to Rwanda. Landrace (LL) sows had significantly smaller (P<0.05) litter sizes at birth (9.04±0.72) compared to LP sows (11.49±0.45). On the other hand, the litter size for LL (9.04±0.58) and LP (11.49±0.67) sires did not differ, while PP sires had the highest (P<0.05) litter size (13.37±1.43). Interestingly, the method of insemination, whether use of a boar or artificially did not (P>0.05) affect the number of inseminations per conception, litter size, and proportion of piglets born live. Sow parity was found to have a linear relationship with a mean litter size at birth of 6.9±0.43 piglets for primiparous sows and 15.2±1.12 piglets for a sow of parity 7. However, the number of piglets born dead increased with parity, peaking at 3.0±0.66 (parity 7), while it was only 0.2±0.2 for parity 1. Therefore, the LP crossbred sows and Pietran sires are recommended. Artificial insemination should be promoted since it performed as well as natural insemination but provides other advantages such as African swine fever and inbreeding prevention, and avoids boar management costs.

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Effect of interval between inseminations when using frozen-thawed boar sperm on fertility and fetal paternity in mature gilts

Cited by 20 publications

References 44 publications

Enhanced fertility prediction of cryopreserved boar spermatozoa using novel sperm function assessment

Enhanced fertility prediction of cryopreserved boar spermatozoa using novel sperm function assessment

Multiple proteins from ejaculated and epididymal porcine spermatozoa bind glycan motifs found in the oviduct

Success drivers of pig artificial insemination based on imported fresh semen

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