Effect of interval-training exercise on subchondral bone in a chemically-induced osteoarthritis model

Boudenot, Arnaud; Presle, Nathalie; Uzbekov, Rustem; Toumi, Hechmi; Pallu, Stéphane; Lespessailles, Éric

doi:10.1016/j.joca.2014.05.020

Cited by 32 publications

(33 citation statements)

References 42 publications

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“…Different MIA doses have been used previously to provoke chemically osteoarthritis in rats (Guzman et al, 2003; Dumond et al, 2004; Boudenot et al, 2014; Guingamp et al, 1997; Miyamoto et al, 2016). However, it is known that beside a rapid and dose-dependent decrease of locomotor activity, a secondary, progressive, long-term loss of spontaneous mobility happens only if high doses of MIA (0.3–3 mg) are applied (Guingamp et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

Meloxicam ameliorates the cartilage and subchondral bone deterioration in monoiodoacetate-induced rat osteoarthritis

Nagy

Vajda

Vari

et al. 2017

PeerJ

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ObjectiveThis study aimed to quantify the cartilage- and subchondral bone-related effects of low-dose and high-dose meloxicam treatment in the late phase of mono-iodoacetate-induced osteoarthritis of the stifle.MethodsThirty-four male Wistar rats received intra-articular injection of mono-iodoacetate to trigger osteoarthritis; 10 control animals (Grp Co) received saline. The mono-iodoacetate-injected rats were assigned to three groups and treated from week 4 to the end of week 7 with placebo (Grp P, n = 11), low-dose (GrpM Lo, 0.2 mg/kg, n = 12) or high-dose (GrpM Hi, 1 mg/kg, n = 11) meloxicam. After a period of 4 additional weeks (end of week 11) the animals were sacrificed, and the stifle joints were examined histologically and immunohistochemically for cyclooxygenase 2, in conformity with recommendations of the Osteoarthritis Research Society International. Serum cytokines IL-6, TNFα and IL-10 were measured at the end of weeks 3, 7, and 11.ResultsCompared with saline-treated controls, animals treated with mono-iodoacetate developed various degrees of osteoarthritis. The cartilage degeneration score and the total cartilage degeneration width were significantly lower in both the low-dose (p = 0.012 and p = 0.014) and high-dose (p = 0.003 and p = 0.006) meloxicam-treated groups than in the placebo group. In the subchondral bone, only high-dose meloxicam exerted a significant protective effect (p = 0.011). Low-grade Cox-2 expression observed in placebo-treated animals was abolished in both meloxicam groups. Increase with borderline significance of TNFα in GrpP from week 3 to week 7 (p = 0.049) and reduction of IL-6 in GrpM Lo from week 3 to week 11 (p = 0.044) were observed.ConclusionIn this rat model of osteoarthritis, both low-dose and high-dose meloxicam had a chondroprotective effect, and the high dose also protected against subchondral bone lesions. The results suggest a superior protection of the high-dose meloxicam arresting the low-grade inflammatory pathway accompanied by chronic cartilage deterioration.

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Section: Discussionmentioning

confidence: 99%

Meloxicam ameliorates the cartilage and subchondral bone deterioration in monoiodoacetate-induced rat osteoarthritis

Nagy

Vajda

Vari

et al. 2017

PeerJ

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“…1b) of the medial compartment (Boudenot et al, 2014). Cores were drawn on the first and last slices and projected and adjusted to fill intervening slices.…”

Section: Methodsmentioning

confidence: 99%

“…Volumetric shapes include strips (Batiste et al, 2004), cylinders (Boudenot et al, 2014; Iijima et al, 2015; Intema et al, 2010; McErlain et al, 2008; Sniekers et al, 2008), and cubes (Florea et al, 2015). Sizes vary from small 2 x 2 x 4mm 3 cubes (Florea et al, 2015) to large anatomical contours that include the medial or lateral compartments of the tibia (Hamann et al, 2012; Maerz et al, 2016; Mohan et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Sizes vary from small 2 x 2 x 4mm 3 cubes (Florea et al, 2015) to large anatomical contours that include the medial or lateral compartments of the tibia (Hamann et al, 2012; Maerz et al, 2016; Mohan et al, 2016). ROI location and number varies from a single load bearing location (Batiste et al, 2004; Boudenot et al, 2014) to multiple locations throughout the compartment (McErlain et al, 2008). The methods can broadly be categorized into 1) single load bearing location (strips, cores or cubes), 2) multiple load bearing locations (triplet cores) and 3) large contours (whole compartment or quadrants).…”

Section: Introductionmentioning

confidence: 99%

“…The study objective was to measure the bone volume fraction of ACL transected and ACL intact knees using three ROIs: 1) the “Whole Compartment Method” (Bouxsein et al, 2010; Hamann et al, 2012), 2) the “Single Core Method” (Boudenot et al, 2014), and 3) the “Triplet Core Method” (McErlain et al, 2008). We hypothesized that these methods would detect similar differences in the bone volume fractions within the medial tibial compartment following ACL transection compared to the contralateral ACL intact (control) knee five weeks post-operatively in the rat.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of micro-CT post-processing methods for evaluating the trabecular bone volume fraction in a rat ACL-transection model

Chin

Karamchedu

Patel

et al. 2016

Journal of Biomechanics

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Trabecular bone volume fraction assessments are likely sensitive to the analysis method and selection of the region of interest. Currently, there are several methods for selecting the region of interest to analyze trabecular bone in animal models of post-traumatic osteoarthritis. The objective of this study was to compare three published methods for determining the trabecular bone volume fraction of the medial tibial epiphyses in ACL transected and contralateral ACL intact knees. Micro-computed tomography images of both knees were obtained five weeks postoperatively and evaluated using three methods: (1) the Whole Compartment Method that captured the entire medial compartment, (2) the centrally located Single Core Method, and (3) the Triplet Core Method that averaged focal locations in the anterior, central, and posterior regions. The Whole Compartment method detected significant bone loss in the ACL transected knee compared to the ACL intact knee (p<0.001), with a loss of 15.2±3.9%. The Single Core and the Triplet Core Methods detected losses of 7.5±10.5% (p=0.061) and 14.1±13.7%(p=0.01), respectively. Details regarding segmentation methods are important for facilitating comparisons between studies, and for selecting methods to document trabecular bone changes and treatment outcomes. Based on these findings, the Whole Compartment Method is recommended, as it was least variable and more sensitive for detecting differences in the bone volume fraction in the medial compartment.

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Effects of Treadmill Exercise on Advanced Osteoarthritis Pain in Rats

Allen

Imbert

Havelin

et al. 2017

Arthritis & Rheumatology

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Objective Exercise is commonly recommended for patients with osteoarthritis (OA) pain. However, whether exercise is beneficial in ameliorating persistent NSAID-resistant ongoing pain associated with advanced OA is unknown. Methods Rats treated with intra-articular monosodium iodoacetate (MIA) or saline underwent treadmill exercise or remained sedentary starting 10 days post-injection. Tactile sensory thresholds and weight bearing were assessed followed by radiographs at weekly intervals. After 4 weeks of exercise, ongoing pain was assessed using conditioned place preference (CPP) to intra-articular or rostral ventromedial medulla (RVM) lidocaine. The possible role of endogenous opioids in exercise-induced pain relief was examined by systemic administration of naloxone. Knee joints were collected for μCT analysis to examine pathological changes to subchondral bone and metaphysis of the tibia. Results Treadmill exercise for 4 weeks reversed MIA-induced tactile hypersensitivity and weight asymmetry. Both intra-articular and RVM lidocaine D35 post-MIA induced CPP in sedentary but not exercised MIA-treated rats, indicating that exercise blocks MIA-induced ongoing pain. Naloxone re-established weight asymmetry in MIA-treated rats undergoing exercise and induced conditioned place aversion (CPA), indicating exercise-induced pain relief is dependent on endogenous opioids. Exercise did not alter radiographic evidence of OA. However, μCT analysis indicates that exercise blocked MIA-induced medial, but not lateral subchondral bone loss and trabecular bone loss in the metaphysis. Conclusion These findings support the conclusion that exercise induces pain relief in advanced, NSAID resistant OA, likely through increased endogenous opioid signaling. In addition, treadmill exercise blocked MIA-induced bone loss in this model, indicating a potential bone stabilizing effect of exercise on the OA joint.

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Effect of interval-training exercise on subchondral bone in a chemically-induced osteoarthritis model

Abstract: Our results suggest that preliminary interval-training improved BMD and osteocytes lacunar occupancy in subchondral bone. Our interval-training did not prevent MIA-induced cartilage degeneration.

Cited by 32 publications

References 42 publications

Meloxicam ameliorates the cartilage and subchondral bone deterioration in monoiodoacetate-induced rat osteoarthritis

Meloxicam ameliorates the cartilage and subchondral bone deterioration in monoiodoacetate-induced rat osteoarthritis

Comparison of micro-CT post-processing methods for evaluating the trabecular bone volume fraction in a rat ACL-transection model

Effects of Treadmill Exercise on Advanced Osteoarthritis Pain in Rats

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