Effect of intestinal tapeworms on the gut microbiota of the common carp, Cyprinus carpio

Fu, Pei P.; Xiong, Fan; Feng, Wen W.; Zou, Hong; Wu, Shan; Li, Ming; Wang, Gui T.; Li, Wen X.

doi:10.1186/s13071-019-3510-z

Cited by 30 publications

(21 citation statements)

References 65 publications

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“…The gastrointestinal microbiota of diseased farmed yellowtail kingfish was dominated by an uncultured Mycoplasmataceae sp. Members of this family are typically found in the gut of farmed fish including Atlantic salmon, rainbow trout and common carp [48][49][50]. In our study, we found that this member was prevalent in diseased fish (both at the early and late stage of gut enteritis) when compared to healthy fish, indicating a potential harmful effect of these bacteria.…”

Section: Discussionsupporting

confidence: 50%

“…The gastrointestinal microbiota of diseased farmed yellowtail kingfish was dominated by an uncultured Mycoplasmataceae sp. Members of this family are typically found in the gut of farmed fish including Atlantic salmon, rainbow trout and common carp [48][49][50]. In our study, we found that this Each polygon represents an ontology term, with their size corresponding to the numbers of genes involved in the associated pathway.…”

Section: Discussionmentioning

confidence: 65%

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Investigating Both Mucosal Immunity and Microbiota in Response to Gut Enteritis in Yellowtail Kingfish

et al. 2020

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The mucosal surfaces of fish play numerous roles including, but not limited to, protection against pathogens, nutrient digestion and absorption, excretion of nitrogenous wastes and osmotic regulation. During infection or disease, these surfaces act as the first line of defense, where the mucosal immune system interacts closely with the associated microbiota to maintain homeostasis. This study evaluated microbial changes across the gut and skin mucosal surfaces in yellowtail kingfish displaying signs of gut inflammation, as well as explored the host gene expression in these tissues in order to improve our understanding of the underlying mechanisms that contribute to the emergence of these conditions. For this, we obtained and analyzed 16S rDNA and transcriptomic (RNA-Seq) sequence data from the gut and skin mucosa of fish exhibiting different health states (i.e., healthy fish and fish at the early and late stages of enteritis). Both the gut and skin microbiota were perturbed by the disease. More specifically, the gastrointestinal microbiota of diseased fish was dominated by an uncultured Mycoplasmataceae sp., and fish at the early stage of the disease showed a significant loss of diversity in the skin. Using transcriptomics, we found that only a few genes were significantly differentially expressed in the gut. In contrast, gene expression in the skin differed widely between health states, in particular in the fish at the late stage of the disease. These changes were associated with several metabolic pathways that were differentially expressed and reflected a weakened host. Altogether, this study highlights the sensitivity of the skin mucosal surface in response to gut inflammation.

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Section: Discussionsupporting

confidence: 50%

“…The gastrointestinal microbiota of diseased farmed yellowtail kingfish was dominated by an uncultured Mycoplasmataceae sp. Members of this family are typically found in the gut of farmed fish including Atlantic salmon, rainbow trout and common carp [48][49][50]. In our study, we found that this Each polygon represents an ontology term, with their size corresponding to the numbers of genes involved in the associated pathway.…”

Section: Discussionmentioning

confidence: 65%

Investigating Both Mucosal Immunity and Microbiota in Response to Gut Enteritis in Yellowtail Kingfish

et al. 2020

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“…As the dominant constituent here in poor performing fish, the occurrence of a single ASV related to an uncultured Mycoplasmataceae raises questions around its association with disease. As a member of this bacterial family, Mycoplasma have been previously identified in the gut of other fish such as Atlantic salmon, Chinook salmon (Oncorhynchus tshawytscha), zebrafish (Danio rerio), common carp (Cyprinus carpio), largemouth bronze gudgeon (Coreius guichenoti) and rainbow trout [38][39][40][41][42][43][44]. While recent genome reconstruction indicates a mutualistic lifestyle of this organism in the intestine of certain species like Atlantic salmon and hadal snailfish (Pseudoliparis swirei) [45,46], for others it has been associated with disease [42].…”

Section: Discussionmentioning

confidence: 99%

Antibiotic-induced alterations and repopulation dynamics of yellowtail kingfish microbiota

et al. 2020

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Background: The use of antibiotics in aquaculture is a common infection treatment and is increasing in some sectors and jurisdictions. While antibiotic treatment can negatively shift gut bacterial communities, recovery and examination of these communities in fish of commercial importance is not well documented. Examining the impacts of antibiotics on farmed fish microbiota is fundamental for improving our understanding and management of healthy farmed fish. This work assessed yellowtail kingfish (Seriola lalandi) skin and gut bacterial communities after an oral antibiotic combination therapy in poor performing fish that displayed signs of enteritis over an 18-day period. In an attempt to promote improved bacterial re-establishment after antibiotic treatment, faecal microbiota transplantation (FMT) was also administered via gavage or in the surrounding seawater, and its affect was evaluated over 15 days post-delivery. Results: Antibiotic treatment greatly perturbed the global gut bacterial communities of poor-performing fishan effect that lasted for up to 18 days post treatment. This perturbation was marked by a significant decrease in species diversity and evenness, as well as a concomitant increase in particular taxa like an uncultured Mycoplasmataceae sp., which persisted and dominated antibiotic-treated fish for the entire 18-day period. The skinassociated bacterial communities were also perturbed by the antibiotic treatment, notably within the first 3 days; however, this was unlike the gut, as skin microbiota appeared to shift towards a more 'normal' (though disparate) state after 5 days post antibiotic treatment. FMT was only able to modulate the impacts of antibiotics in some individuals for a short time period, as the magnitude of change varied substantially between individuals. Some fish maintained certain transplanted gut taxa (i.e. present in the FMT inoculum; namely various Aliivibrio related ASVs) at Day 2 post FMT, although these were lost by Day 8 post FMT. Conclusion: As we observed notable, prolonged perturbations induced by antibiotics on the gut bacterial assemblages, further work is required to better understand the processes/dynamics of their re-establishment following antibiotic exposure. In this regard, procedures like FMT represent a novel approach for promoting improved microbial recovery, although their efficacy and the factors that support their success requires further investigation.

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“…As the dominant constituent here in poor performing sh, the occurrence of a single ASV related to an uncultured Mycoplasmataceae raises questions around its association with disease. As a member of this bacterial family, Mycoplasma have been previously identi ed in the gut of other sh such as Atlantic salmon, Chinook salmon (Oncorhynchus tshawytscha), zebra sh (Danio rerio), common carp (Cyprinus carpio), largemouth bronze gudgeon (Coreius guichenoti) and rainbow trout [38][39][40][41][42][43][44]. While recent genome reconstruction indicates a mutualistic lifestyle of this organism in the intestine of certain species like Atlantic salmon and hadal snail sh (Pseudoliparis swirei) [45,46], for others it has been associated with disease [42].…”

Section: Discussionmentioning

confidence: 99%

Antibiotic-induced alterations and repopulation dynamics of yellowtail kingfish microbiota

Legrand

Catalano

Wos‐Oxley

et al. 2020

Preprint

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Background The use of antibiotics in aquaculture is a common infection treatment and is increasing in some sectors and jurisdictions. While antibiotic treatment can negatively shift gut bacterial communities, recovery and examination of these communities in fish of commercial importance is not well documented. Examining the impacts of antibiotics on farmed fish microbiota is fundamental for improving our understanding and management of healthy farmed fish. This work assessed yellowtail kingfish ( Seriola lalandi ) skin and gut bacterial communities after an oral antibiotic combination therapy in poor performing fish that displayed signs of enteritis over an 18-day period. In an attempt to promote improved bacterial re-establishment after antibiotic treatment, faecal microbiota transplantation (FMT) was also administered via gavage or in the surrounding seawater, and its affect was evaluated over 15 days post-delivery. Results Antibiotic treatment greatly perturbed the global gut bacterial communities of poor-performing fish – an effect that lasted for up to 18 days post treatment. This perturbation was marked by a significant decrease in species diversity and evenness, as well as a concomitant increase in particular taxa like an uncultured Mycoplasmataceae sp., which persisted and dominated antibiotic-treated fish for the entire 18-day period. The skin-associated bacterial communities were also perturbed by the antibiotic treatment, notably within the first 3 days; however, this was unlike the gut, as skin microbiota appeared to shift towards a more ‘normal’ (though disparate) state after 5 days post antibiotic treatment. FMT was only able to modulate the impacts of antibiotics in some individuals for a short time period, as the magnitude of change varied substantially between individuals. Some fish maintained certain transplanted gut taxa (i.e. present in the FMT inoculum; namely various Aliivibrio related ASVs) at Day 2 post FMT, although these were lost by Day 8 post FMT. Conclusion As we observed notable, prolonged perturbations induced by antibiotics on the gut bacterial assemblages, further work is required to better understand the processes/dynamics of their re-establishment following antibiotic exposure. In this regard, procedures like FMT represent a novel approach for promoting improved microbial recovery, although their efficacy and the factors that support their success requires further investigation.

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Effect of intestinal tapeworms on the gut microbiota of the common carp, Cyprinus carpio

Cited by 30 publications

References 65 publications

Investigating Both Mucosal Immunity and Microbiota in Response to Gut Enteritis in Yellowtail Kingfish

Investigating Both Mucosal Immunity and Microbiota in Response to Gut Enteritis in Yellowtail Kingfish

Antibiotic-induced alterations and repopulation dynamics of yellowtail kingfish microbiota

Antibiotic-induced alterations and repopulation dynamics of yellowtail kingfish microbiota

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